Chemical Derivatization of Peptide Carboxyl Groups for Highly Efficient Electron Transfer Dissociation
Frey BL, Ladror DT, Sondalle SB, Krusemark CJ, Jue AL, Coon JJ, Smith LM. Chemical Derivatization of Peptide Carboxyl Groups for Highly Efficient Electron Transfer Dissociation. Journal Of The American Society For Mass Spectrometry 2013, 24: 1710-1721. PMID: 23918461, PMCID: PMC3827969, DOI: 10.1007/s13361-013-0701-2.Peer-Reviewed Original ResearchConceptsElectron transfer dissociationTransfer dissociationBovine serum albuminTryptic digestCarboxyl groupsLabeling reagentPeptide carboxyl groupsETD-MS/MSComplex peptide mixturesIncreased charge densitySequence coverageAlkyl tertiary aminesHigh sequence coverageAdditional peptide identificationsDerivatization strategyDerivatized peptidesPeptide derivatizationChemical derivatizationPeptide ionsPrecursor ionsPeptide mixturesETD fragmentationPeptide spectral matchesReaction efficiencyTertiary amines