2008
Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery
Mansfield K, Lang S, Gauduin M, Sanford H, Lifson J, Johnson R, Desrosiers R. Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery. Journal Of Virology 2008, 82: 4135-4148. PMID: 18272584, PMCID: PMC2292994, DOI: 10.1128/jvi.00015-08.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralInterferon-gammaIntestinal MucosaLymphoid TissueMacaca mulattaNeutralization TestsRetroviridae ProteinsRNA, ViralSAIDS VaccinesSequence DeletionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusT-LymphocytesVaccines, AttenuatedViral Envelope ProteinsViral LoadViremiaConceptsAnti-SIV antibody responsesSimian immunodeficiency virusAntibody responseImmune responseVaccine protectionImmunodeficiency virusCopies/Gamma interferon enzyme-linked immunospotVaccine/challenge experimentsAttenuated simian immunodeficiency virusGut-associated lymphoid tissueHigh-titer antibody responsesAnti-SIV immune responsesEnzyme-linked immunospotMacrophage marker CD68Cellular immune responsesHumoral immune responseViral load measurementsWeeks of infectionNeutralization-resistant strainDouble-labeling experimentsVaccinated monkeysViral loadPeripheral bloodLymphoid tissue
2006
Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus
Kaur A, Sanford H, Garry D, Lang S, Klumpp S, Watanabe D, Bronson R, Lifson J, Rosati M, Pavlakis G, Felber B, Knipe D, Desrosiers R. Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus. Virology 2006, 357: 199-214. PMID: 16962628, PMCID: PMC1819472, DOI: 10.1016/j.virol.2006.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralGenetic VectorsMacaca mulattaSAIDS VaccinesSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusSimplexvirusVaccinationVaccines, DNAConceptsSimian immunodeficiency virusELISPOT responsesImmunodeficiency virusHSV vectorsHerpes simplex virus vectorsPlasma SIV RNAPlasma viremia levelsVector-based vaccinesDay of challengeSimplex virus vectorsNef fusion proteinRecombinant HSV vectorsSIV RNAIntravenous challengeViremia levelsAntibody titersVaccine approachesImmune responseRhesus macaquesProtective capacityMacaquesRecombinant herpesvirusDNA vectorsFusion proteinEnv
2005
Immunization of Macaques with Single-Cycle Simian Immunodeficiency Virus (SIV) Stimulates Diverse Virus-Specific Immune Responses and Reduces Viral Loads after Challenge with SIVmac239
Evans D, Bricker J, Sanford H, Lang S, Carville A, Richardson B, Piatak M, Lifson J, Mansfield K, Desrosiers R. Immunization of Macaques with Single-Cycle Simian Immunodeficiency Virus (SIV) Stimulates Diverse Virus-Specific Immune Responses and Reduces Viral Loads after Challenge with SIVmac239. Journal Of Virology 2005, 79: 7707-7720. PMID: 15919923, PMCID: PMC1143664, DOI: 10.1128/jvi.79.12.7707-7720.2005.Peer-Reviewed Original ResearchMeSH KeywordsAIDS VaccinesAnimalsAntibodies, ViralCD8-Positive T-LymphocytesCell LineImmunizationInterferon-gammaLymphocyte ActivationMacaca mulattaRNA, ViralSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusViral LoadConceptsSingle-Cycle Simian Immunodeficiency VirusesRNA copies/AIDS vaccine approachSingle-cycle SIVEnzyme-linked immunospotViral loadSimian immunodeficiency virusCopies/Immunodeficiency virusVaccine approachesGamma interferon enzyme-linked immunospotGag-specific T-cell responsesVirus-specific immune responsesMajor Histocompatibility Complex Class I DownregulationT cell proliferative responsesImmunization of macaquesPlasma viral loadT cell responsesMHC class IThird doseChronic phasePeripheral bloodWeek 8Immune responseControl animals
2004
Impact of Nef-Mediated Downregulation of Major Histocompatibility Complex Class I on Immune Response to Simian Immunodeficiency Virus
Swigut T, Alexander L, Morgan J, Lifson J, Mansfield K, Lang S, Johnson R, Skowronski J, Desrosiers R. Impact of Nef-Mediated Downregulation of Major Histocompatibility Complex Class I on Immune Response to Simian Immunodeficiency Virus. Journal Of Virology 2004, 78: 13335-13344. PMID: 15542684, PMCID: PMC525019, DOI: 10.1128/jvi.78.23.13335-13344.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesDown-RegulationHistocompatibility Antigens Class IMacaca mulattaSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusViral Regulatory and Accessory ProteinsConceptsSimian immunodeficiency virusHuman immunodeficiency virusImmunodeficiency virusWeeks postinfectionMajor Histocompatibility Complex Class I DownregulationClass IMajor histocompatibility complex class IHistocompatibility complex class IComplex class IMHC class INef gene productParental wild-type virusImmune responseLymphocyte activationWild-type virusNef functionCD4 downregulationFunctional activityMHC downregulationDownregulationResponse-4VirusMonkeysPostinfectionNef
1995
Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys
Du Z, Lang S, Sasseville V, Lackner A, Ilyinskii P, Daniel M, Jung J, Desrosiers R. Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys. Cell 1995, 82: 665-674. PMID: 7664345, DOI: 10.1016/0092-8674(95)90038-1.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAllelesAmino Acid SequenceAnimalsBase SequenceDNA PrimersDNA, ViralGene Products, envGene Products, nefGenes, nefLymphocyte ActivationMacaca mulattaMiceMolecular Sequence DataPhagocytesPhenotypePhosphorylationRetroviridae Proteins, OncogenicSignal TransductionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransformation, GeneticTyrosineViral Fusion ProteinsVirus ReplicationConceptsAcute diseasePeripheral blood mononuclear cell culturesBlood mononuclear cell culturesMononuclear cell culturesT lymphocyte activationSevere diarrheaLymphoid proliferationsGastrointestinal tractNef allelesMacaque monkeysLymphocyte activationCellular activationSIVmac239NefDiseaseActivationMonkeysCell culturesImportant determinantCellsNIH 3T3 cellsTyrosine phosphorylationCotransfected COS cellsCOS cellsDiarrheavpr deletion mutant of simian immunodeficiency virus induces AIDS in rhesus monkeys
Hoch J, Lang S, Weeger M, Stahl-Hennig C, Coulibaly C, Dittmer U, Hunsmann G, Fuchs D, Müller J, Sopper S. vpr deletion mutant of simian immunodeficiency virus induces AIDS in rhesus monkeys. Journal Of Virology 1995, 69: 4807-4813. PMID: 7609047, PMCID: PMC189293, DOI: 10.1128/jvi.69.8.4807-4813.1995.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGenes, vprMacaca mulattaPoint MutationSequence DeletionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusProgression to AIDS in the absence of a gene for vpr or vpx
Gibbs J, Lackner A, Lang S, Simon M, Sehgal P, Daniel M, Desrosiers R. Progression to AIDS in the absence of a gene for vpr or vpx. Journal Of Virology 1995, 69: 2378-2383. PMID: 7884883, PMCID: PMC188910, DOI: 10.1128/jvi.69.4.2378-2383.1995.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralCell LineGenes, vprMacaca mulattaMolecular Sequence DataOligodeoxyribonucleotidesPhenotypeSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusViral Regulatory and Accessory ProteinsConceptsLow virus burdenSimian immunodeficiency virusVirus burdenRhesus monkeysLymphocyte concentrationsPlasma antigenemiaHigh virus burdenLymph node morphologyImmunodeficiency virusDisease progressionSIVmac replicationPrimate lentivirusesVirus replicationWild-type virusNode morphologyAIDSVpx geneTerminal stageProgressionAntigenemiaCD4VpxMonkeysEarly spikeBurden