Featured Publications
Kaposi’s Sarcoma-Associated Herpesvirus K3 and K5 Proteins Down Regulate Both DC-SIGN and DC-SIGNR
Lang SM, Bynoe MO, Karki R, Tartell MA, Means RE. Kaposi’s Sarcoma-Associated Herpesvirus K3 and K5 Proteins Down Regulate Both DC-SIGN and DC-SIGNR. PLOS ONE 2013, 8: e58056. PMID: 23460925, PMCID: PMC3584050, DOI: 10.1371/journal.pone.0058056.Peer-Reviewed Original ResearchAmino Acid MotifsCell Adhesion MoleculesCell MembraneDown-RegulationEndocytosisHEK293 CellsHerpesvirus 8, HumanHumansImmunoprecipitationLectins, C-TypeLysosomesProteasome Endopeptidase ComplexProtein StabilityProtein Structure, TertiaryProteolysisReceptors, Cell SurfaceStructure-Activity RelationshipTyrosineUbiquitinationViral Proteins
2000
Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stolte N, Stahl-Hennig C, Mätz-Rensing K, Fuchs D, Skowronski J, Kirchhoff F. Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques. Journal Of Virology 2000, 74: 4155-4164. PMID: 10756028, PMCID: PMC111930, DOI: 10.1128/jvi.74.9.4155-4164.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCD4 AntigensCell Line, TransformedCOS CellsDown-RegulationGene Products, nefHistocompatibility Antigens Class IHumansMacaca mulattaMutagenesisP21-Activated KinasesPhosphorylationProtein Serine-Threonine KinasesSimian immunodeficiency virusSrc Homology DomainsTyrosineViral LoadVirus ReplicationConceptsAbility of NefTyrosine-based endocytosis signalTyrosine residuesHigh viral loadCellular signal transductionTyrosine kinase SrcNef functionViral loadEndocytosis signalEndocytic machineryPXXP motifN-terminal tyrosine residueCell surface expressionKinase SrcKinase assaysSignal transductionProline residuesClass I major histocompatibility complexN-terminal tyrosineI major histocompatibility complexLigand domainCombined mutationsNef interactionsMajor histocompatibility complexMutations
1997
Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques
Lang S, Lafrate A, Stahl-Hennig C, Kuhn E, Nisslein T, Kaup F, Haupt M, Hunsmann G, Skowronski J, Kirchhoff F. Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques. Nature Medicine 1997, 3: 860-865. PMID: 9256276, DOI: 10.1038/nm0897-860.Peer-Reviewed Original ResearchConceptsCellular serine/threonine kinaseSerine/threonine kinaseThreonine kinaseTyrosine kinase SrcPutative SH3Simian immunodeficiency virus NefKinase SrcMutant formsEfficient replicationKinaseInduction of AIDSSimian immunodeficiency virusNef geneCD4 downregulationSIVmac NefRhesus macaquesRapid deathNef sequencesSH3NefSrcGenesDomainDifferent time pointsPathogenicity
1995
Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗)
Jung J, Lang S, Friedrich U, Jun T, Roberts T, Desrosiers R, Biesinger B. Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗). Journal Of Biological Chemistry 1995, 270: 20660-20667. PMID: 7544793, DOI: 10.1074/jbc.270.35.20660.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCell LineChlorocebus aethiopsDNA PrimersHerpesvirus 2, SaimiriineLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMutagenesis, Site-DirectedPeptide FragmentsPhosphoproteinsPhosphoserinePhosphothreoninePhosphotyrosinePoint MutationPolymerase Chain ReactionProtein-Tyrosine KinasesRatsRecombinant ProteinsSequence Homology, Amino AcidSpodopteraStructure-Activity RelationshipTransfectionTyrosineViral ProteinsConceptsC-terminal SrcCellular protein kinasesBinding of sequencesInteraction of TipHerpesvirus saimiriLck kinase activityCOS-1 cellsRat-1 cell lineLck functionPutative SH3Cellular proteinsSH3 bindingProtein kinaseCarboxyl terminusLck kinaseVivo associationKinase activityMutational analysisFusion proteinLckAmino acidsKinaseHuman T cellsProteinCell linesIdentification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys
Du Z, Lang S, Sasseville V, Lackner A, Ilyinskii P, Daniel M, Jung J, Desrosiers R. Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys. Cell 1995, 82: 665-674. PMID: 7664345, DOI: 10.1016/0092-8674(95)90038-1.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAllelesAmino Acid SequenceAnimalsBase SequenceDNA PrimersDNA, ViralGene Products, envGene Products, nefGenes, nefLymphocyte ActivationMacaca mulattaMiceMolecular Sequence DataPhagocytesPhenotypePhosphorylationRetroviridae Proteins, OncogenicSignal TransductionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransformation, GeneticTyrosineViral Fusion ProteinsVirus ReplicationConceptsAcute diseasePeripheral blood mononuclear cell culturesBlood mononuclear cell culturesMononuclear cell culturesT lymphocyte activationSevere diarrheaLymphoid proliferationsGastrointestinal tractNef allelesMacaque monkeysLymphocyte activationCellular activationSIVmac239NefDiseaseActivationMonkeysCell culturesImportant determinantCellsNIH 3T3 cellsTyrosine phosphorylationCotransfected COS cellsCOS cellsDiarrhea