2022
Alterations in high‐dimensional T‐cell profile and gene signature of immune aging in HIV‐infected older adults without viremia
Shin MS, Park H, Salahuddin S, Montgomery RR, Emu B, Shaw AC, Kang I. Alterations in high‐dimensional T‐cell profile and gene signature of immune aging in HIV‐infected older adults without viremia. Aging Cell 2022, 21: e13702. PMID: 36036630, PMCID: PMC9577958, DOI: 10.1111/acel.13702.Peer-Reviewed Original ResearchConceptsOlder human immunodeficiency virusPeripheral blood mononuclear cellsHuman immunodeficiency virusAntiretroviral therapyT cellsDetectable viremiaMemory CD8HIV infectionAge-associated immune alterationsYoung human immunodeficiency virusReplication-competent HIV-1Combination antiretroviral therapyEffector memory CD8T-cell countsSubset of CD4T cell profileBlood mononuclear cellsAgeing-associated genesEM CD8Immune alterationsMemory CD4Immune agingImmunodeficiency virusInflammatory moleculesMononuclear cells
2019
Multi‐site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system
Bagwell CB, Hunsberger B, Hill B, Herbert D, Bray C, Selvanantham T, Li S, Villasboas JC, Pavelko K, Strausbauch M, Rahman A, Kelly G, Asgharzadeh S, Gomez‐Cabrero A, Behbehani G, Chang H, Lyberger J, Montgomery R, Zhao Y, Inokuma M, Goldberger O, Stelzer G. Multi‐site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system. Cytometry Part B Clinical Cytometry 2019, 98: 146-160. PMID: 31758746, PMCID: PMC7543682, DOI: 10.1002/cyto.b.21858.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsWhole bloodPeripheral blood mononuclear cell preparationsDeep immune phenotypingBlood mononuclear cellsImmune cell populationsMononuclear cell preparationsCell populationsTranslational clinical researchWhole blood preparationsImmune phenotypingMononuclear cellsPBMC samplesClinical trialsImmune cellsClinical researchCyTOF technologyBlood preparationsInter-site reproducibilityBloodCell preparationsSingle donorMulti-site reproducibilityCellsProfiling assaysTranscriptomic analysis of human IL-7 receptor alphalow and higheffector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults
Park H, Shin M, Kim M, Bilsborrow J, Mohanty S, Montgomery R, Shaw A, Yoo S, Kang I. Transcriptomic analysis of human IL-7 receptor alphalow and higheffector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults. The Journal Of Immunology 2019, 202: 181.22-181.22. DOI: 10.4049/jimmunol.202.supp.181.22.Peer-Reviewed Original ResearchPeripheral blood cellsPeripheral blood mononuclear cellsInfluenza vaccine responsesT cellsEM CD8Memory CD8Vaccine responsesOlder adultsBlood cellsSignature genesEffector memory CD8Blood mononuclear cellsHuman peripheral whole bloodAge-associated expansionPeripheral whole bloodGene expression profilesAge-associated changesMeta-analysis studyGlobal transcriptomic profilesMononuclear cellsCD8Expression profilesSignificant associationTranscription factorsWhole bloodTranscriptomic analysis of human IL‐7 receptor alpha low and high effector memory CD8+ T cells reveals an age‐associated signature linked to influenza vaccine response in older adults
Park H, Shin MS, Kim M, Bilsborrow JB, Mohanty S, Montgomery RR, Shaw AC, You S, Kang I. Transcriptomic analysis of human IL‐7 receptor alpha low and high effector memory CD8+ T cells reveals an age‐associated signature linked to influenza vaccine response in older adults. Aging Cell 2019, 18: e12960. PMID: 31044512, PMCID: PMC6612637, DOI: 10.1111/acel.12960.Peer-Reviewed Original ResearchConceptsPeripheral blood cellsPeripheral blood mononuclear cellsEffector memory CD8Influenza vaccine responsesEM CD8T cellsIL-7RαMemory CD8Vaccine responsesBlood cellsOlder adultsIL-7 receptor alphaSignature genesBlood mononuclear cellsHuman peripheral whole bloodAge-associated expansionPeripheral whole bloodGene expression profilesAge-associated changesMeta-analysis studyGlobal transcriptomic profilesMononuclear cellsCD8Receptor alphaExpression profiles
2002
Human phagocytic cells in the early innate immune response to Borrelia burgdorferi
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Human phagocytic cells in the early innate immune response to Borrelia burgdorferi. The Journal Of Infectious Diseases 2002, 185: 1773-1779. PMID: 12085324, DOI: 10.1086/340826.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesImmune responseEarly innate immune responseKilling of spirochetesSpecific antibodiesBorrelia burgdorferiSecondary immune responseInnate immune responseInnate immune systemHuman phagocytic cellsSpirochete clearanceMononuclear cellsImmune systemLyme diseasePhagocytic cellsNatural infectionMature macrophagesSpirochetesIntracellular colocalizationAntibodiesBurgdorferiFirst cellsLimited uptakeCellsMonocytes