2020
Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF
Stewart E, Wang X, Chupp GL, Montgomery RR. Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF. Journal Of Leukocyte Biology 2020, 108: 1555-1564. PMID: 32911570, PMCID: PMC8087109, DOI: 10.1002/jlb.5ma0720-770rr.Peer-Reviewed Original ResearchConceptsAirway immune cellsSource of inflammationChronic inflammatory diseaseInflammatory cell typesStudy of pathogenesisMultiple distinct subtypesHeterogeneity of diseaseBronchial epithelial cellsAirway inflammationCell typesAsthmatic patientsClinical responseEosinophilic infiltrateAnalysis of sputumAsthmatic inflammationFunctional statusClinical managementImmune cellsInflammatory diseasesHealthy controlsInflammatory responseAirway cellsSputumDistinct subtypesInflammation
2017
Multiparameter Single Cell Profiling of Airway Inflammatory Cells
Yao Y, Welp T, Liu Q, Niu N, Wang X, Britto CJ, Krishnaswamy S, Chupp G, Montgomery RR. Multiparameter Single Cell Profiling of Airway Inflammatory Cells. Cytometry Part B Clinical Cytometry 2017, 92: 12-20. PMID: 27807928, PMCID: PMC5250532, DOI: 10.1002/cyto.b.21491.Peer-Reviewed Original ResearchConceptsSingle-cell methodsComplex trait diseasesSubgroup of asthmaticsSingle-cell analysisMultiparameter single cell analysisMillions of patientsTranscriptional analysisImmunologic underpinningsInduced sputumAirway diseaseAsthmatic patientsAirway samplesCell subsetsImmune statusFunctional statusClinical severityDistinct biologic mechanismsTreatment successPhysiologic manifestationsBiologic mechanismsCystic fibrosisCellular analysisPatientsCytometry studiesU.S. population