1995
Regulation of Transgenic Class II Major Histocompatibility Genes in Murine Langerhans Cells
Longley J, Ding T, Levin D, Lewis J, Edelson R, Tigelaar R, Flavell R. Regulation of Transgenic Class II Major Histocompatibility Genes in Murine Langerhans Cells. Journal Of Investigative Dermatology 1995, 104: 329-334. PMID: 7860996, DOI: 10.1111/1523-1747.ep12665365.Peer-Reviewed Original ResearchConceptsSplenic dendritic cellsEpidermal Langerhans cellsLangerhans cellsDendritic cellsE expressionClass II major histocompatibility complex moleculesLangerhans cells expressionMurine Langerhans cellsMajor histocompatibility complex moleculesNear-normal levelsB6 genetic backgroundHistocompatibility complex moleculesMajor histocompatibility complex class II gene expressionImmune functionClass II proteinsImmunoperoxidase stainingCell-specific regulatory sequencesClass II gene expressionTransgenic miceCell expressionImmunofluorescence cytometryIntact animalsDecrease expressionMajor histocompatibility genesHistocompatibility genes
1990
TCR γ/δ+ Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid Tissue
Tigelaar R, Lewis J, Bergstresser P. TCR γ/δ+ Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid Tissue. Journal Of Investigative Dermatology 1990, 94: s58-s63. PMID: 1972173, DOI: 10.1111/1523-1747.ep12875138.Peer-Reviewed Original ResearchConceptsDendritic epidermal T cellsGamma/delta cellsT cell antigen receptorEpidermal T cellsT cellsCD4-CD8Alpha/beta T cell antigen receptorGamma/delta typeSkin-Associated Lymphoid TissueBone marrow-derived cellsGamma/delta populationsClass II MHC moleculesDelta cellsMarrow-derived cellsTCR junctional regionsEarly fetal thymocytesMHC-like moleculesLangerhans cellsLymphoid tissueTCR heterogeneityNormal miceMHC moleculesThy-1Fetal thymocytesMHC typeLangerhans cells that migrate to skin after intravenous infusion regulate the induction of contact hypersensitivity.
Cruz P, Tigelaar R, Bergstresser P. Langerhans cells that migrate to skin after intravenous infusion regulate the induction of contact hypersensitivity. The Journal Of Immunology 1990, 144: 2486-92. PMID: 1969448, DOI: 10.4049/jimmunol.144.7.2486.Peer-Reviewed Original ResearchConceptsLow-dose UVB radiationContact hypersensitivityLangerhans cellsIntravenous infusionLow-dose ultraviolet B (UVB) radiationExposure of LCThy-1UVB radiationSuppressor signalsMesenteric lymph nodesLow-dose UVBEffector signalsSkin graft experimentsLC migrationImmunologic targetsLymph nodesAllogeneic transferCH responsesLymphoid tissueImmune responseSyngeneic miceImmunization modelImmunogenic propertiesInfusionUltraviolet B radiation
1988
γδ Antigen receptors of Thy-1 + dendritic epidermal cells: Implications for thymic differentiation
Allison J, Hayran W, Asarnow D, Tigelaar R, Tucker P, Bonyhadi M. γδ Antigen receptors of Thy-1 + dendritic epidermal cells: Implications for thymic differentiation. Immunologic Research 1988, 7: 292. PMID: 2906352, DOI: 10.1007/bf02935534.Peer-Reviewed Original ResearchAnimalsAntigens, Differentiation, T-LymphocyteAntigens, SurfaceBase SequenceCD3 ComplexCell DifferentiationDendritic CellsDNAGene Expression RegulationGene Rearrangement, gamma-Chain T-Cell Antigen ReceptorGene Rearrangement, T-LymphocyteMiceReceptors, Antigen, T-CellReceptors, Antigen, T-Cell, gamma-deltaT-LymphocytesThy-1 AntigensEffect of Keratinocyte Cytokines on Thy‐1+ Dendritic Epidermal Cells
TIGELAAR R, NIXON‐FULTON J, TAKASHIMA A, KUZIEL W, TAKIJIRI C, LEWIS J, TUCKER P, BERGSTRESSER P. Effect of Keratinocyte Cytokines on Thy‐1+ Dendritic Epidermal Cells. Annals Of The New York Academy Of Sciences 1988, 548: 271-282. PMID: 2470301, DOI: 10.1111/j.1749-6632.1988.tb18815.x.Peer-Reviewed Original ResearchThy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression.
Nixon-Fulton J, Kuziel W, Santerse B, Bergstresser P, Tucker P, Tigelaar R. Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression. The Journal Of Immunology 1988, 141: 1897-903. PMID: 2902136, DOI: 10.4049/jimmunol.141.6.1897.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceBlotting, NorthernBlotting, SouthernCell DivisionConcanavalin ADendritic CellsEpidermal CellsEpidermisEthersFemaleInterleukin-2IonomycinMaleMiceMice, Inbred BALB CMice, Inbred CBAMice, NudeReceptors, Antigen, T-CellSpecies SpecificityTetradecanoylphorbol AcetateThy-1 AntigensThymus GlandConceptsIL-2Thy-1Nude miceNormal miceLow-dose IL-2BALB/c miceT cell receptor expressionModest proliferative responseThymus-bearing miceCell receptor expressionT cell differentiationTCR-gamma transcriptsC miceReceptor expressionAthymic miceProliferative responseNormal animalsTCR mRNACalcium ionophoreEpidermal cellsMiceNormal CBACon AFollicular epitheliumPopulation of cellsPhenotypic Heterogeneity and Cytotoxic Activity of Con A and IL-2-Stimulated Cultures of Mouse Thy-1+ Epidermal Cells
Nixon-Fulton J, Hackett J, Bergstresser P, Kumar V, Tigelaar R. Phenotypic Heterogeneity and Cytotoxic Activity of Con A and IL-2-Stimulated Cultures of Mouse Thy-1+ Epidermal Cells. Journal Of Investigative Dermatology 1988, 91: 62-68. PMID: 2898506, DOI: 10.1111/1523-1747.ep12463290.Peer-Reviewed Original ResearchConceptsAntibody-dependent cell-mediated cytotoxicityYAC-1 targetsLyt-2Thy-1Asialo GM1IL-2Con AMouse Thy-1IL-2-stimulated culturesCytotoxic activityDendritic Thy-1NK-sensitive targetsNK-resistant targetsCell-mediated cytotoxicityT-cell markersShort-term cultured cellsPhenotypic heterogeneityMaximum cytotoxic activityYAC-1Interleukin-2Flow cytometryL3T4Population of cellsMouse epidermisLong-term cultureThy-1+ Dendritic Epidermal Cells in Mice: Precursor Frequency Analysis and Cloning of Concanavalin A-Reactive Cells
Takashima A, Nixon-Fulton J, Bergstresser P, Tigelaar R. Thy-1+ Dendritic Epidermal Cells in Mice: Precursor Frequency Analysis and Cloning of Concanavalin A-Reactive Cells. Journal Of Investigative Dermatology 1988, 90: 671-678. PMID: 2896217, DOI: 10.1111/1523-1747.ep12560835.Peer-Reviewed Original ResearchConceptsDendritic epidermal cellsPrecursor frequency analysisIL-2Lyt-2Thy-1Con AGrowth factorExogenous IL-2Higher proliferative responsesFunctional activityCytotoxic activityYAC-1Precursor frequencyProliferative responseMouse Thy-1Dilution microculturesLow cytotoxic activityHigh cytotoxic activityTarget cellsLow responsivenessCells/Vivo activityBulk culturesFiller cellsEpidermal cells
1987
Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells
Kuziel W, Takashima A, Bonyhadi M, Bergstresser P, Allison J, Tigelaar R, Tucker P. Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. Nature 1987, 328: 263-266. PMID: 2885757, DOI: 10.1038/328263a0.Peer-Reviewed Original ResearchConceptsEpidermal cellsT cell receptorDendritic epidermal cellsThy-1Steady-state levelsMurine Thy-1T lymphocytesCell differentiationT-cell lineageT cell differentiationDistinct populationsMost T lymphocytesRNA expressionMajor histocompatibility complexΔ complexNecessary eventΑ-chainΓ-chainMinor populationDendritic cellsLangerhans cellsExpressionCellsNormal miceHistocompatibility complexLack of dendritic Thy-1+ epidermal cells in mice with severe combined immunodeficiency disease.
Nixon-Fulton J, Witte P, Tigelaar R, Bergstresser P, Kumar V. Lack of dendritic Thy-1+ epidermal cells in mice with severe combined immunodeficiency disease. The Journal Of Immunology 1987, 138: 2902-5. PMID: 2883236, DOI: 10.4049/jimmunol.138.9.2902.Peer-Reviewed Original ResearchConceptsDendritic Thy-1SCID miceEpidermal cell suspensionsThy-1NK cellsT lymphocytesEpidermal whole mountsSevere combined immunodeficiency diseaseCombined immunodeficiency diseaseNormal bone marrowControl miceImmunodeficiency diseaseBone marrowWhole mountsMiceImmunofluorescent stainingCon ACell suspensionsEC suspensionsEpidermal cellsLymphocytesPrecursor levelsCellsTransplantationMarrow
1986
Induction and regulation of contact hypersensitivity by resident, bone marrow-derived, dendritic epidermal cells: Langerhans cells and Thy-1+ epidermal cells.
Sullivan S, Bergstresser P, Tigelaar R, Streilein J. Induction and regulation of contact hypersensitivity by resident, bone marrow-derived, dendritic epidermal cells: Langerhans cells and Thy-1+ epidermal cells. The Journal Of Immunology 1986, 137: 2460-7. PMID: 2876041, DOI: 10.4049/jimmunol.137.8.2460.Peer-Reviewed Original ResearchConceptsContact hypersensitivityBone marrow-derived populationLangerhans cellsThy-1Epicutaneous applicationInduction of CHSRegulation of CHSDendritic epidermal cellsRoute of immunizationEpidermal Langerhans cellsSpecific unresponsivenessAntigenic encounterCH responsesImmunogenic signalsSyngeneic miceImmunogenic capacityAntigenic signalIntact skinMouse skinCellular elementsEpidermal cellsHypersensitivityMiceCell typesInductionThy-1+ epidermal cells proliferate in response to concanavalin A and interleukin 2.
Nixon-Fulton J, Bergstresser P, Tigelaar R. Thy-1+ epidermal cells proliferate in response to concanavalin A and interleukin 2. The Journal Of Immunology 1986, 136: 2776-86. PMID: 2870120, DOI: 10.4049/jimmunol.136.8.2776.Peer-Reviewed Original ResearchConceptsPeripheral T cellsIL-2Langerhans cellsRat IL-2T cellsThy-1Con AAnti-T cell reagentsMature peripheral T cellsAKR/J miceComplement depletion studiesT-cell markersConcanavalin AFluorescence-activated cell sorterThy-1 antigenNK cellsMice 20Lyt-1Lyt-2J miceInterleukin-2Maximal responseMouse epidermal cellsAsialo GM1Day 5
1985
Origin and Function of Thy-1+ Dendritic Epidermal Cells in Mice
Bergstresser P, Sullivan S, Streilein J, Tigelaar R. Origin and Function of Thy-1+ Dendritic Epidermal Cells in Mice. Journal Of Investigative Dermatology 1985, 85: s85-s90. PMID: 2409184, DOI: 10.1111/1523-1747.ep12275516.Peer-Reviewed Original ResearchConceptsLangerhans cellsFluorescence Activated Cell SorterDendritic epidermal cellsNatural suppressor cellsNatural killer cellsAntigen-presenting cellsEpidermal Langerhans cellsCells/mm2Bone marrow originThy-1 antigenNormal mouse skinCell surface determinantsBone marrow cellsSuppressor cellsCutaneous sitesDendritic cellsKiller cellsMonoclonal antibody 20T lymphocytesLymphoreticular cellsMarrow originAsialo GM1Vascular sourceThy-1Marrow cells
1984
Thy-1 Antigen-Bearing Dendritic Cells in Murine Epidermis Are Derived from Bone Marrow Precursors
Bergstresser P, Tigelaar R, Streilein J. Thy-1 Antigen-Bearing Dendritic Cells in Murine Epidermis Are Derived from Bone Marrow Precursors. Journal Of Investigative Dermatology 1984, 83: 83-87. PMID: 6147381, DOI: 10.1111/1523-1747.ep12262587.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntigens, SurfaceBone MarrowBone Marrow CellsBone Marrow TransplantationCell MovementEpidermal CellsEpidermisFluorescent Antibody TechniqueHematopoietic Stem CellsIsoantibodiesLangerhans CellsMiceMice, Inbred AKRMice, Inbred DBARadiation ChimeraSkin TransplantationThy-1 AntigensConceptsLangerhans cellsBone marrow precursorsThy-1 antigenMurine epidermisThy-1Thy-1.2Anti-I-Ad antibodiesMarrow precursorsRecipient-derived cellsBone marrow chimerasDendritic Langerhans cellsNormal skin epidermisNormal murine epidermisAKR recipientsGraft epidermisDendritic cellsHematogenous cellsDonor originSystemic availabilityAKR miceSame graftLethal irradiationLymphoid cellsAbdominal skinGraft
1983
Thy-1 Antigen-Bearing Dendritic Cells Populate Murine Epidermis
Bergstresser P, Tigelaar R, Dees J, Streilein J. Thy-1 Antigen-Bearing Dendritic Cells Populate Murine Epidermis. Journal Of Investigative Dermatology 1983, 81: 286-288. PMID: 6136547, DOI: 10.1111/1523-1747.ep12518332.Peer-Reviewed Original ResearchConceptsLangerhans cellsMurine epidermisCutaneous T-cell lymphomaEar epidermisDendritic cell populationsStrain BALB/cT-cell lymphomaEpidermal Langerhans cellsThy-1.2 antigenBALB/cCell populationsThy-1 antigenT lymphocytesIa antigensDistinct cell populationsMalignant counterpartsThy-1Monoclonal antibodiesStrain B10.AAntigenMelanocytesImmunofluorescence microscopySkin surfaceEpidermisMature melanocytes