2019
Potentiated serotonin signaling in serotonin re‐uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function
Greig CJ, Zhang L, Cowles RA. Potentiated serotonin signaling in serotonin re‐uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function. Physiological Reports 2019, 7: e14278. PMID: 31724827, PMCID: PMC6854605, DOI: 10.14814/phy2.14278.Peer-Reviewed Original ResearchConceptsIntestinal absorptive functionEnterocyte massCrypt proliferation indexSmall intestineAbsorptive functionDistal bowelSmall bowelCrypt depthSERT knockoutVillus heightProliferative parametersSmall intestinal absorptive functionPlasma citrulline levelsCrypt cell proliferationPeptide absorptionSignificant increaseMucosal surface areaAttractive therapeutic targetFunctional intestinal mucosaWT miceCitrulline levelsPlasma citrullineSerotonin systemIntestinal mucosaLuminal instillation
2016
Enhanced serotonin signaling increases intestinal neuroplasticity
Greig CJ, Gandotra N, Tackett JJ, Bamdad MC, Cowles RA. Enhanced serotonin signaling increases intestinal neuroplasticity. Journal Of Surgical Research 2016, 206: 151-158. PMID: 27916355, DOI: 10.1016/j.jss.2016.07.021.Peer-Reviewed Original ResearchConceptsSelective serotonin reuptake inhibitorsIntestinal mucosal growthSERT knockoutMucosal growthEnterocyte proliferationEnteric nervous systemSerotonin reuptake inhibitorsMassive bowel resectionSerotonin reuptake transporterAge-matched WTGAP-43 expressionWild-type miceSynthesis inhibitionMarkers of neuroplasticityImportant regulatorReversal of effectsEpithelial cell compartmentWestern blot analysisBowel resectionReuptake inhibitorsWT miceReuptake transporterMucosal homeostasisIntestinal segmentsCrypt depth
2012
Neuronal Serotonin Regulates Growth of the Intestinal Mucosa in Mice
Gross ER, Gershon MD, Margolis KG, Gertsberg ZV, Li Z, Cowles RA. Neuronal Serotonin Regulates Growth of the Intestinal Mucosa in Mice. Gastroenterology 2012, 143: 408-417.e2. PMID: 22609381, PMCID: PMC3687781, DOI: 10.1053/j.gastro.2012.05.007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCholine O-AcetyltransferaseCholinergic NeuronsEnterochromaffin CellsIntestinal MucosaKetanserinMaleMiceMice, Inbred C57BLMice, KnockoutMuscarinic AntagonistsReceptor, Serotonin, 5-HT2AScopolamineSelective Serotonin Reuptake InhibitorsSerotonergic NeuronsSerotoninSerotonin AntagonistsSerotonin Plasma Membrane Transport ProteinsTryptophan HydroxylaseConceptsSelective serotonin re-uptake inhibitorsSerotonin re-uptake inhibitorsRe-uptake inhibitorsWild-type miceIntestinal mucosaSerotonin re-uptake transporterCholinergic submucosal neuronsIntestinal mucosal epitheliumRe-uptake transporterTryptophan hydroxylase 1Tryptophan hydroxylase 2Submucosal neuronsCholinergic neuronsCholine acetyltransferaseNeuronal serotoninMucosal growthMucosal epitheliumMucosal cellsSERT knockoutEpithelial proliferationAbstractTextMiceHydroxylase 2MucosaNeural precursors