2021
ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus
Yao J, Leng L, Fu W, Li J, Bronner C, Bucala R. ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus. Arthritis & Rheumatology 2021, 73: 1931-1942. PMID: 33844457, DOI: 10.1002/art.41753.Peer-Reviewed Original ResearchMeSH KeywordsAllelesApoptosisCCAAT-Enhancer-Binding ProteinsCell LineFibroblastsGene Expression RegulationGenetic LociGenetic Predisposition to DiseaseGenotypeGlucocorticoidsHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsPromoter Regions, GeneticReceptors, GlucocorticoidUbiquitin-Protein LigasesConceptsMacrophage migration inhibitory factorAutoimmune disease severityMIF -794 CATTRheumatoid synovial fibroblastsMIF expressionGlucocorticoid receptorGlucocorticoid sensitivitySynovial fibroblastsT cellsDisease severityPeripheral blood T cellsBlood T cellsMigration inhibitory factorGlucocorticoid-treated cellsAP-1Glucocorticoid immunosuppressionMIF promoterMIF genotypeMIF polymorphismsFactor antibodyInflammatory responseT lymphocytesActivator protein-1Glucocorticoid responsivenessInhibitory factor
2002
Macrophage Migration Inhibitory Factor: A Critical Neurohumoral Mediator
Petrovsky N, Bucala R. Macrophage Migration Inhibitory Factor: A Critical Neurohumoral Mediator. Frontiers Of Hormone Research 2002, 29: 83-90. PMID: 11789350, DOI: 10.1159/000061060.Peer-Reviewed Original Research
2001
Neuroendocrine properties of macrophage migration inhibitory factor (MIF)
Fingerle‐Rowson G, Bucala R. Neuroendocrine properties of macrophage migration inhibitory factor (MIF). Immunology And Cell Biology 2001, 79: 368-375. PMID: 11488984, DOI: 10.1046/j.1440-1711.2001.01024.x.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorInhibitory factorCytokine macrophage migration inhibitory factorPro-inflammatory actionsMIF expressionSeptic shockNeuroendocrine mediatorsInflammatory diseasesPathogenic roleEndocrine circuitsNeuroendocrine propertiesImmune systemEndocrine tissuesImmune tissuesNeuroendocrine mechanismsDiseaseTissueArthritisNeuroendocrineFactors
2000
Regulation of macrophage migration inhibitory factor by endogenous glucocorticoids in rat adjuvant‐induced arthritis
Leech M, Metz C, Bucala R, MorAnd E. Regulation of macrophage migration inhibitory factor by endogenous glucocorticoids in rat adjuvant‐induced arthritis. Arthritis & Rheumatism 2000, 43: 827-833. PMID: 10765927, DOI: 10.1002/1529-0131(200004)43:4<827::aid-anr13>3.0.co;2-k.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorEnzyme-linked immunosorbent assaySerum MIF levelsMigration inhibitory factorMIF levelsEndogenous glucocorticoidsADX ratsSham operationRegulation of MIFInhibitory factorIsotype-matched control antibodyTumor necrosis factor alphaAdjuvant arthritis inductionClinical disease parametersReversal of diseaseNecrosis factor alphaSites of inflammationArthritis inductionArthritis developmentMIF productionMIF releaseControl antibodyInflammatory diseasesSignificant exacerbationSupernatant levels
1999
Macrophage migration inhibitory factor in rheumatoid arthritis: Evidence of proinflammatory function and regulation by glucocorticoids
Leech M, Metz C, Hall P, Hutchinson P, Gianis K, Smith M, Weedon H, Holdsworth S, Bucala R, Morand E. Macrophage migration inhibitory factor in rheumatoid arthritis: Evidence of proinflammatory function and regulation by glucocorticoids. Arthritis & Rheumatism 1999, 42: 1601-1608. PMID: 10446857, DOI: 10.1002/1529-0131(199908)42:8<1601::aid-anr6>3.0.co;2-b.Peer-Reviewed Original ResearchMeSH KeywordsAgedArthritis, RheumatoidCells, CulturedCytokinesGlucocorticoidsHumansImmunohistochemistryInterferon-gammaLymphocyte ActivationMacrophage Migration-Inhibitory FactorsMacrophagesMonocytesReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSynovial MembraneT-LymphocytesTumor Necrosis Factor-alphaConceptsMacrophage migration inhibitory factorFibroblast-like synoviocytesRA fibroblast-like synoviocytesEnzyme-linked immunosorbent assayRheumatoid arthritisMigration inhibitory factorTNFalpha releaseExpression of MIFIntracellular macrophage migration inhibitory factorInhibitory factorCultured RA fibroblast-like synoviocytesCytokine macrophage migration inhibitory factorCultured fibroblast-like synoviocytesTumor necrosis factor-alpha synthesisHuman RA synoviumRA synovial tissueNecrosis factor-alpha synthesisHuman autoimmune diseasesPotential therapeutic targetTranscription-polymerase chain reactionMessenger RNA expressionT cell activationMIF expressionRA patientsRA synovium
1998
Reversal of Established Rat Crescentic Glomerulonephritis by Blockade of Macrophage Migration Inhibitory Factor (MIF): Potential Role of MIF in Regulating Glucocorticoid Production
Yang N, Nikolic-Paterson D, Ng Y, Mu W, Metz C, Bacher M, Meinhardt A, Bucala R, Atkins R, Lan H. Reversal of Established Rat Crescentic Glomerulonephritis by Blockade of Macrophage Migration Inhibitory Factor (MIF): Potential Role of MIF in Regulating Glucocorticoid Production. Molecular Medicine 1998, 4: 413-424. PMID: 10780884, PMCID: PMC2230272, DOI: 10.1007/bf03401748.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorCrescentic glomerulonephritisDay 7Ab treatmentRenal functionControl AbInhibitory factorPotent pro-inflammatory cytokineEndogenous glucocorticoid levelsIsotype control AbSevere renal injuryNormal renal functionCellular immune responsesPro-inflammatory cytokinesReversal of diseaseSerum corticosterone levelsExperimental crescentic glomerulonephritisInterleukin-1 productionRat crescentic glomerulonephritisCrescentic diseaseProgressive glomerulonephritisRenal injuryGBM glomerulonephritisLeukocyte infiltrationNeuroimmunomodulation by Macrophage Migration Inhibitory Factor (MIF)
BUCALA R. Neuroimmunomodulation by Macrophage Migration Inhibitory Factor (MIF). Annals Of The New York Academy Of Sciences 1998, 840: 74-82. PMID: 9629239, DOI: 10.1111/j.1749-6632.1998.tb09551.x.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorImmune responseRole of MIFInhibitory factorT cell cytokine productionAnti-inflammatory propertiesAnterior pituitary cellsT-lymphocyte factorSteroid therapyLymph nodesCytokine productionImmunosuppressive effectsInflammatory conditionsGlucocorticoid actionT cellsFirst cytokineInflammatory sitesTherapeutic strategiesProinflammatory stimuliPituitary cellsImmune systemLymphocyte factorsGlucocorticoidsInhibitory effectRegulation of the immune response by macrophage migration inhibitory factor: biological and structural features
Bernhagen J, Calandra T, Bucala R. Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features. Journal Of Molecular Medicine 1998, 76: 151-161. PMID: 9535548, DOI: 10.1007/s001090050204.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorInhibitory factorCytokine macrophage migration inhibitory factorDelayed-type hypersensitivity reactionNegative septic shockB cell responsesMIF productionSeptic shockImmunosuppressive effectsHypersensitivity reactionsImmune responseImmunological functionsCell responsesHost macrophagesCritical mediatorGlucocorticoidsMediatorsBiological roleResponseCytokine structureCytokinesDiseaseMacrophagesStress response
1997
Biochemical and Mutational Investigations of the Enzymatic Activity of Macrophage Migration Inhibitory Factor †
Bendrat K, Al-Abed Y, Callaway D, Peng T, Calandra T, Metz C, Bucala R. Biochemical and Mutational Investigations of the Enzymatic Activity of Macrophage Migration Inhibitory Factor †. Biochemistry 1997, 36: 15356-15362. PMID: 9398265, DOI: 10.1021/bi971153a.Peer-Reviewed Original ResearchConceptsEnzymatic activityL-dopachrome methyl esterN-terminal proline residueSite-directed mutagenesisAmino acid residuesDopachrome tautomerase activityNative solution conditionsMutant proteinsProtein domainsStable trimer formationCarboxy terminusBasic residuesN-terminusProline residuesSDS-PAGE analysisBacterial enzymesHomotrimeric structureAcid residuesCatalytic functionEnzymatic mechanismMutational investigationTrimer formationLow protein concentrationsBiochemical analysisInternal histidineMacrophage migration inhibitory factor: a regulator of glucocorticoid activity with a critical role in inflammatory disease
Donnelly S, Bucala R. Macrophage migration inhibitory factor: a regulator of glucocorticoid activity with a critical role in inflammatory disease. Trends In Molecular Medicine 1997, 3: 502-507. PMID: 9430786, DOI: 10.1016/s1357-4310(97)01133-7.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorAcute respiratory distress syndromeRelease of MIFFunction of MIFEfficacy of glucocorticoidsRespiratory distress syndromeAnti-inflammatory effectsMigration inhibitory factorEffects of glucocorticoidsAnterior pituitary glandARDS pathogenesisDistress syndromeGlucocorticoid activityInflammatory diseasesT cellsFirst cytokineInflammatory sitesPituitary glandInhibitory factorGlucocorticoidsCritical rolePhysiological responsesCytokinesPathogenesisSyndromeMacrophage Migration Inhibitory Factor (MIF): A Glucocorticoid Counter-Regulator within the Immune System
Calandra T, Bucala R. Macrophage Migration Inhibitory Factor (MIF): A Glucocorticoid Counter-Regulator within the Immune System. Critical Reviews In Immunology 1997, 17: 77-88. PMID: 9034724, DOI: 10.1615/critrevimmunol.v17.i1.30.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCattleChickensGene Expression RegulationGlucocorticoidsHumansInterferon-gammaInterleukinsLipopolysaccharidesMacrophage Migration-Inhibitory FactorsMacrophagesMiceMolecular Sequence DataMolecular StructurePituitary GlandRatsSequence Homology, Amino AcidShock, SepticT-LymphocytesTumor Necrosis Factor-alphaConceptsMacrophage migration inhibitory factorMigration inhibitory factorImmune responseImmune systemT lymphocyte-derived factorInhibitory factorInhibitory effectLymphocyte-derived factorRecombinant MIF proteinIFN-gamma productionIL-1 betaIL-8 productionT cell proliferationAnterior pituitary glandEnigmatic cytokineLethal endotoxemiaIL-6MIF proteinIL-2TNF-alphaCounter regulatorT lymphocytesProtective effectGlucocorticoid inhibitionPituitary glandRole of Macrophage Migration Inhibitory Factor in the Regulation of the Immune Response
Metz C, Bucala R. Role of Macrophage Migration Inhibitory Factor in the Regulation of the Immune Response. Advances In Immunology 1997, 66: 197-223. PMID: 9328642, DOI: 10.1016/s0065-2776(08)60598-2.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorT cellsImmune responseInhibitory factorAnti-inflammatory effectsInflammatory disease statesAnterior pituitary glandSeptic shockMIF actionMIF proteinInflammatory responseGlucocorticoid actionImmune systemPituitary glandCorticotrophic cellsGlucocorticoidsInhibitory effectDisease statesBaseline statePotential roleMacrophagesCellsResponseArthritis
1996
MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid‐induced regulator of the immune response
Bucala R. MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid‐induced regulator of the immune response. The FASEB Journal 1996, 10: 1607-1613. PMID: 9002552, DOI: 10.1096/fasebj.10.14.9002552.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorImmune responseSecretion of MIFMigration inhibitory factorAnterior pituitary cellsImmune cell activationT-lymphocyte productsProfound inhibitory effectFirst cytokine activitiesCounterregulatory hormonesLymph nodesCytokine productionImmunosuppressive effectsGlucocorticoid actionLymphocyte productsT cellsPituitary hormonesInflammatory sitesCytokine activityPituitary cellsSteroid concentrationsCell activationImmune systemInhibitory factorInhibitory effectMIF re-discovered: Pituitary hormone and glucocorticoid-induced regulator of cytokine production
Bucala R. MIF re-discovered: Pituitary hormone and glucocorticoid-induced regulator of cytokine production. Cytokine & Growth Factor Reviews 1996, 7: 19-24. PMID: 8864351, DOI: 10.1016/1359-6101(96)00008-1.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorAssociation of MIFT cell-derived factorsCounter-regulatory hormonesPrimary immune responseMigration inhibitory factorCell-derived factorSource of infectionAnterior pituitary cell lineFirst cytokine activitiesLymph nodesCytokine productionGlucocorticoid actionPituitary cell lineImmune responsePituitary hormonesInflammatory sitesCytokine activityHost responseImmune systemInhibitory factorInhibitory effectTissue invasionProtein mediatorsRandom migration
1995
MIF as a glucocorticoid-induced modulator of cytokine production
Calandra T, Bernhagen J, Metz C, Spiegel L, Bacher M, Donnelly T, Cerami A, Bucala R. MIF as a glucocorticoid-induced modulator of cytokine production. Nature 1995, 377: 68-71. PMID: 7659164, DOI: 10.1038/377068a0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell LineCytokinesDexamethasoneEnzyme-Linked Immunosorbent AssayFemaleGlucocorticoidsHumansHydrocortisoneImmunityInflammationLipopolysaccharidesMacrophage ActivationMacrophage Migration-Inhibitory FactorsMacrophagesMiceMice, Inbred BALB CRatsRats, Sprague-DawleyRecombinant ProteinsShock, SepticT-LymphocytesConceptsMacrophage migration inhibitory factorCounter-regulatory systemsMigration inhibitory factorProtein macrophage migration inhibitory factorGlucocorticoid protectionLethal endotoxaemiaMIF productionCytokine productionCytokine secretionMacrophage cytokinesEndogenous mediatorsImmunosuppressive propertiesImmune responseGlucocorticoid hormonesInhibitory factorHormonal systemsCritical mediatorUnexpected findingMediatorsVital functionsEndotoxaemiaCytokinesGlucocorticoidsMonocytesPituitary
1985
Non‐enzymatic modification of proteins by steroids: Pathological implications for autoimmunity and glucocorticoid toxicity
Bucala R, Cerami A. Non‐enzymatic modification of proteins by steroids: Pathological implications for autoimmunity and glucocorticoid toxicity. BioEssays 1985, 3: 55-59. PMID: 3842583, DOI: 10.1002/bies.950030204.Peer-Reviewed Original ResearchConceptsOral contraceptive agentsElevated plasma levelsHigh steroid levelsGlucocorticoid toxicityPlasma levelsCovalent addition productsPathological sequelaeContraceptive agentsSteroid levelsCertain steroidsAddition productsSteroidsPathological implicationsRearrangement productsMicrosomal oxidationNon-enzymatic modificationGlucocorticoid-lens protein adducts in experimentally induced steroid cataracts
Bucala R, Callati M, Manabe S, Cotlier E, Cerami A. Glucocorticoid-lens protein adducts in experimentally induced steroid cataracts. Experimental Eye Research 1985, 40: 853-863. PMID: 4018168, DOI: 10.1016/0014-4835(85)90130-7.Peer-Reviewed Original Research