2022
Targeting fibrocytes in autoimmunity
Bucala RJ. Targeting fibrocytes in autoimmunity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2121739119. PMID: 35086933, PMCID: PMC8812540, DOI: 10.1073/pnas.2121739119.Peer-Reviewed Original Research
2021
ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus
Yao J, Leng L, Fu W, Li J, Bronner C, Bucala R. ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus. Arthritis & Rheumatology 2021, 73: 1931-1942. PMID: 33844457, DOI: 10.1002/art.41753.Peer-Reviewed Original ResearchMeSH KeywordsAllelesApoptosisCCAAT-Enhancer-Binding ProteinsCell LineFibroblastsGene Expression RegulationGenetic LociGenetic Predisposition to DiseaseGenotypeGlucocorticoidsHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsPromoter Regions, GeneticReceptors, GlucocorticoidUbiquitin-Protein LigasesConceptsMacrophage migration inhibitory factorAutoimmune disease severityMIF -794 CATTRheumatoid synovial fibroblastsMIF expressionGlucocorticoid receptorGlucocorticoid sensitivitySynovial fibroblastsT cellsDisease severityPeripheral blood T cellsBlood T cellsMigration inhibitory factorGlucocorticoid-treated cellsAP-1Glucocorticoid immunosuppressionMIF promoterMIF genotypeMIF polymorphismsFactor antibodyInflammatory responseT lymphocytesActivator protein-1Glucocorticoid responsivenessInhibitory factor
2001
Fibrocytes induce an angiogenic phenotype in cultured endothelial cells and promote angiogenesis in vivo
Hartlapp I, Abe R, Saeed R, Peng T, Voelter W, Bucala R, Metz C. Fibrocytes induce an angiogenic phenotype in cultured endothelial cells and promote angiogenesis in vivo. The FASEB Journal 2001, 15: 2215-2224. PMID: 11641248, DOI: 10.1096/fj.01-0049com.Peer-Reviewed Original ResearchConceptsBlood vessel formationAngiogenic phenotypeVessel formationMesenchymal cell typesEndothelial cell invasionEndothelial cellsExtracellular matrix-degrading enzymesEndothelial cell migrationGrowth factorCellular microenvironmentMatrix-degrading enzymesCell invasionCell migrationCell typesCultured endothelial cellsTube formationHematopoietic growth factorsPromotion of angiogenesisPhenotypeAngiogenesis modelMicrovascular endothelial cellsCultured fibrocytesEnzymeAngiogenesisVivoPeripheral Blood Fibrocytes: Differentiation Pathway and Migration to Wound Sites
Abe R, Donnelly S, Peng T, Bucala R, Metz C. Peripheral Blood Fibrocytes: Differentiation Pathway and Migration to Wound Sites. The Journal Of Immunology 2001, 166: 7556-7562. PMID: 11390511, DOI: 10.4049/jimmunol.166.12.7556.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood CellsCell DifferentiationCell MovementCells, CulturedCollagenFemaleFibroblastsFibrosisGelsHumansInjections, IntravenousLipopolysaccharide ReceptorsMiceMice, Inbred BALB CReceptors, ChemokineStem Cell TransplantationStem CellsTransforming Growth Factor betaTransforming Growth Factor beta1Wound HealingConceptsCultured fibrocytesTissue injuryChemokine/chemokine receptor interactionsUnique cell surface phenotypeCutaneous tissue injurySecondary lymphoid chemokineAlpha-smooth muscle actinWound healingWound healing myofibroblastsMononuclear cell populationsCCR7 chemokine receptorChemokine receptor interactionsPotent immunostimulatory activitySmooth muscle actinCell surface phenotypeBlood-borne cellsDifferentiation pathwayFibrocyte traffickingLymphoid chemokinesFibrocyte differentiationChemokine receptorsT cellsSurface phenotypePotent stimulusMuscle actin
2000
Peripheral blood fibrocytes: Mesenchymal precursor cells and the pathogenesis of fibrosis
Chesney J, Bucala R. Peripheral blood fibrocytes: Mesenchymal precursor cells and the pathogenesis of fibrosis. Current Rheumatology Reports 2000, 2: 501-505. PMID: 11123104, DOI: 10.1007/s11926-000-0027-5.Peer-Reviewed Original ResearchConceptsPeripheral blood fibrocytesBlood fibrocytesTissue injuryDistinct cell surface phenotypePersistent T-cell activationNaïve T cellsPathogenesis of fibrosisCell surface phenotypeT cell activationCognate immunityAutoimmune disordersConnective tissue cellsImmunohistochemical studyForeign antigensT cellsSurface phenotypeMesenchymal precursor cellsScar formationFibrotic tissueFibrocytesGrowth factorPrecursor cellsNovel populationMatrix depositionInjury
1999
Peripheral blood fibrocytes with foamy virus infection-like morphology
Grab D, Lanners H, Williams W, Bucala R. Peripheral blood fibrocytes with foamy virus infection-like morphology. Human Pathology 1999, 30: 1395-1396. PMID: 10571524, DOI: 10.1016/s0046-8177(99)90076-x.Peer-Reviewed Original ResearchInteraction of Borrelia burgdorferi with Peripheral Blood Fibrocytes, Antigen-Presenting Cells with the Potential for Connective Tissue Targeting
Grab D, Lanners H, Martin L, Chesney J, Cai C, Adkisson H, Bucala R. Interaction of Borrelia burgdorferi with Peripheral Blood Fibrocytes, Antigen-Presenting Cells with the Potential for Connective Tissue Targeting. Molecular Medicine 1999, 5: 46-54. PMID: 10072447, PMCID: PMC2230375, DOI: 10.1007/bf03402138.Peer-Reviewed Original ResearchConceptsPeripheral blood fibrocytesBlood fibrocytesDifferent cell typesB. burgdorferiB. burgdorferi bindsInteraction of BorreliaMolecular mechanismsOspB proteinsAntigen presenting cellsCell typesFibroblast-like cellsCellular interactionsCell membraneJoint connective tissuesCollagen type ILyme arthritisPresent antigensInflammatory processT cellsFunctional capacityCellular collagenImmune systemFlow cytometryFibrocytesConnective tissue
1997
The peripheral blood fibrocyte is a potent antigen-presenting cell capable of priming naive T cells in situ
Chesney J, Bacher M, Bender A, Bucala R. The peripheral blood fibrocyte is a potent antigen-presenting cell capable of priming naive T cells in situ. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6307-6312. PMID: 9177213, PMCID: PMC21045, DOI: 10.1073/pnas.94.12.6307.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsAntigens, CDCell DifferentiationCell MovementCells, CulturedCicatrixCoculture TechniquesCrosses, GeneticFemaleFibroblastsFlow CytometryHIVHIV Core Protein p24HIV Envelope Protein gp120HLA-DR AntigensHumansImmunophenotypingLymphocyte ActivationMaleMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred DBANeutralization TestsSkinT-LymphocytesConceptsNaive T cellsAntigen presentationT cellsHuman fibrocytesDistinct cell surface phenotypePrime naive T cellsPotent antigen-presenting cellsMajor histocompatability complex (MHC) moleculesAdhesion molecules CD11aAntigen-specific immunityProximal lymph nodesPeripheral blood fibrocytesAntigen-presenting cellsCostimulatory molecules CD80T cell proliferationCell surface phenotypeBlood-borne cellsHIV protein p24Dendritic cellsLymph nodesBlood fibrocytesPotent APCsTissue injurySurface phenotypeCutaneous injury
1994
Circulating Fibrocytes Define a New Leukocyte Subpopulation That Mediates Tissue Repair
Bucala R, Spiegel L, Chesney J, Hogan M, Cerami A. Circulating Fibrocytes Define a New Leukocyte Subpopulation That Mediates Tissue Repair. Molecular Medicine 1994, 1: 71-81. PMID: 8790603, PMCID: PMC2229929, DOI: 10.1007/bf03403533.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBone MarrowBone Marrow CellsCD4 AntigensCell AdhesionCells, CulturedCentrifugationChimeraCollagenConnective TissueCytoskeletonDNA-Binding ProteinsDose-Response Relationship, RadiationFemaleFibroblastsFlow CytometryFluorescent Antibody TechniqueHumansImmunohistochemistryLeukocytesMaleMiceMice, Inbred BALB CMicroscopy, ElectronMolecular Sequence DataNuclear ProteinsPhenotypeSex-Determining Region Y ProteinTime FactorsTranscription FactorsTransplantation, HeterologousVimentinWound HealingConceptsTissue injuryLeukocyte subpopulationsScar formationLong-term remodelingFibroblast-like propertiesNormal wound repairConnective tissue scarConnective tissue elementsCell typesFibrotic responseTissue scarWound chambersPathological fibrotic responsesHost responseInjuryConnective tissueFibrocytesWound repairFibroblast propertiesTissue repairTissue elementsDistinctive phenotypeSubpopulationsTissueNovel cell typesDifferential expression of the small inducible cytokines GRO α and GROβ by synovial fibroblasts in chronic arthritis: Possible role in growth regulation
Hogan M, Sherry B, Ritchlin C, Fabre M, Winchester R, Cerami A, Bucala R. Differential expression of the small inducible cytokines GRO α and GROβ by synovial fibroblasts in chronic arthritis: Possible role in growth regulation. Cytokine 1994, 6: 61-69. PMID: 8003635, DOI: 10.1016/1043-4666(94)90009-4.Peer-Reviewed Original ResearchMeSH KeywordsActinsArthritis, RheumatoidBase SequenceCells, CulturedChemokine CXCL1Chemokines, CXCChemotactic FactorsConsensus SequenceCytokinesDNA PrimersFibroblastsGene ExpressionGrowth SubstancesHumansIntercellular Signaling Peptides and ProteinsInterleukin-8Molecular Sequence DataPolymerase Chain ReactionRNA, MessengerSulfur RadioisotopesSynovial MembraneTranscription, GeneticConceptsSynovial fibroblastsGRO alphaChronic arthritisRheumatoid synoviaSynovial fibroblast cell lineGRO betaPro-inflammatory statePro-inflammatory activityRheumatoid arthritisChronic inflammationConnective tissue responseRheumatoid synoviumInflammatory cytokinesRate of proliferationSynovial pannusCytokine activationGRO gammaMetalloproteinase activityArthritisSynoviaGrowth factorConstitutive expressionGrowth dysregulationTissue responseCell lines