Carboxy Terminus of Human Herpesvirus 8 Latency-Associated Nuclear Antigen Mediates Dimerization, Transcriptional Repression, and Targeting to Nuclear Bodies
Schwam D, Luciano R, Mahajan S, Wong L, Wilson A. Carboxy Terminus of Human Herpesvirus 8 Latency-Associated Nuclear Antigen Mediates Dimerization, Transcriptional Repression, and Targeting to Nuclear Bodies. Journal Of Virology 2000, 74: 8532-8540. PMID: 10954554, PMCID: PMC116365, DOI: 10.1128/jvi.74.18.8532-8540.2000.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, ViralCell LineCell NucleusConserved SequenceDimerizationGene Expression Regulation, ViralGenes, ReporterHerpesvirus 8, HumanHumansImmunoblottingLuciferasesMicroscopy, FluorescenceMolecular Sequence DataNuclear ProteinsSequence AlignmentTranscription, GeneticVirionConceptsLatency-associated nuclear antigenC-terminusHeterologous DNA-binding domainDNA-binding domainForms stable dimersPotent transcriptional repressorViral lytic genesC-terminal regionSeries of truncationsHigher-order multimersViral gene expressionNuclear specklesSubnuclear specklesTranscriptional repressionTranscriptional repressorCellular genesCertain B-cell lymphomasNuclear bodiesActive promotersCarboxy terminusGene expressionCell immortalizationFunctional similarityViral episomesIntact proteinN-terminal transcriptional activation domain of LZIP comprises two LxxLL motifs and the Host Cell Factor-1 binding motif
Luciano R, Wilson A. N-terminal transcriptional activation domain of LZIP comprises two LxxLL motifs and the Host Cell Factor-1 binding motif. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 10757-10762. PMID: 10984507, PMCID: PMC27096, DOI: 10.1073/pnas.190062797.Peer-Reviewed Original ResearchConceptsTranscriptional activation domainHCF-1Activation domainLXXLL motifPotent transcriptional activation domainSequence-specific DNA-binding proteinsN-terminal beta-propeller domainTerminal transcriptional activation domainHost cell factor 1N-terminal 92 amino acidsTranscriptional activator VP16Multiprotein enhancer complexesDNA-binding proteinsProtein-protein interactionsImmediate early gene transcriptionBeta-propeller domainCell factor 1Nuclear hormone receptorsBZIP proteinsEnhancer complexTranscriptional coactivatorGene transcriptionBinding motifLZIPCell cycle