2020
A Membrane-Bound Diacylglycerol Species Induces PKCϵ-Mediated Hepatic Insulin Resistance
Lyu K, Zhang Y, Zhang D, Kahn M, Ter Horst KW, Rodrigues MRS, Gaspar RC, Hirabara SM, Luukkonen PK, Lee S, Bhanot S, Rinehart J, Blume N, Rasch MG, Serlie MJ, Bogan JS, Cline GW, Samuel VT, Shulman GI. A Membrane-Bound Diacylglycerol Species Induces PKCϵ-Mediated Hepatic Insulin Resistance. Cell Metabolism 2020, 32: 654-664.e5. PMID: 32882164, PMCID: PMC7544641, DOI: 10.1016/j.cmet.2020.08.001.Peer-Reviewed Original ResearchConceptsPlasma membraneEndoplasmic reticulumHigh-fat diet-induced hepatic insulin resistanceSubcellular fractionation methodInsulin receptor kinaseKey lipid speciesHepatic insulin resistanceDiet-induced hepatic insulin resistanceReceptor kinaseDiacylglycerol acyltransferase 2Molecular mechanismsAcute knockdownPhosphorylationLipid dropletsLipid speciesAcyltransferase 2KnockdownLiver-specific overexpressionDAG accumulationPKCϵDAG contentMembraneFractionation methodKinaseMitochondria
2019
Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice
Nakandakari S, Muñoz V, Kuga G, Gaspar R, Sant'Ana M, Pavan I, da Silva L, Morelli A, Simabuco F, da Silva A, de Moura L, Ropelle E, Cintra D, Pauli J. Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice. Brain Behavior And Immunity 2019, 79: 284-293. PMID: 30797044, DOI: 10.1016/j.bbi.2019.02.016.Peer-Reviewed Original ResearchConceptsHigh-fat dietYoung miceShort-term HFD feedingMice fed high-fat dietFed high-fat dietHippocampus of miceER stressDisease developmentShort-term consumptionAlzheimer's disease developmentPotential molecular mechanismsHOMA-IRHFD feedingAD markersMicroglial cellsAD pathogenesisRisk factorsInflammatory signalsDiet modulatesΒ-amyloidCleaved caspase3HippocampusApoptosis markersHigh expressionMice
2018
Acute physical exercise increases leptin‐induced hypothalamic extracellular signal‐regulated kinase1/2 phosphorylation and thermogenesis of obese mice
Gaspar R, Muñoz V, Kuga G, Nakandakari S, Minuzzi L, Botezelli J, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Acute physical exercise increases leptin‐induced hypothalamic extracellular signal‐regulated kinase1/2 phosphorylation and thermogenesis of obese mice. Journal Of Cellular Biochemistry 2018, 120: 697-704. PMID: 30206970, DOI: 10.1002/jcb.27426.Peer-Reviewed Original ResearchConceptsAcute physical exerciseBrown adipose tissueObese micePhysical exerciseEnergy expenditureERK1/2 phosphorylationTreatment of obesityExtracellular signal-regulated kinase1/2 (ERK1/2) phosphorylationProtein 1 (UCP1) contentExtracellular signal-regulated kinase 1/2Signal-regulated kinase 1/2Sympathetic signalsSpontaneous activityAdipose tissueP-ERK1/2Main interventionThermoregulatory effectsMiceKey mediatorThermogenesisOxygen uptakeMolecular changesKinase 1/2ObeseObesityAcute physical exercise increases the adaptor protein APPL1 in the hypothalamus of obese mice
Gaspar R, Muñoz V, Formigari G, Kuga G, Nakandakari S, Botezelli J, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Acute physical exercise increases the adaptor protein APPL1 in the hypothalamus of obese mice. Cytokine 2018, 110: 87-93. PMID: 29705396, DOI: 10.1016/j.cyto.2018.04.013.Peer-Reviewed Original ResearchConceptsAcute physical exerciseObese micePhysical exerciseFood intakeArcuate proopiomelanocortin neuronsSerum adiponectin levelsProtein-3 levelsEffects of leptinCentral nervous systemRole of APPL1Proopiomelanocortin neuronsAdiponectin levelsAcute exerciseNervous systemHypothalamusPathophysiological processesAdiponectinAdaptor protein APPL1MiceProtein kinase BLeucine zipper motif 1Kinase BExerciseIntakeProtein contentExercise increases Rho‐kinase activity and insulin signaling in skeletal muscle
Muñoz V, Gaspar R, Kuga G, da Rocha A, Crisol B, Botezelli J, Baptista I, Mekary R, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Exercise increases Rho‐kinase activity and insulin signaling in skeletal muscle. Journal Of Cellular Physiology 2018, 233: 4791-4800. PMID: 29219181, DOI: 10.1002/jcp.26278.Peer-Reviewed Original Research
2017
The role of physical exercise on Sestrin1 and 2 accumulations in the skeletal muscle of mice
Crisol B, Lenhare L, Gaspar R, Gaspar R, Muñoz V, da Silva A, Cintra D, de Moura L, Pauli J, Ropelle E. The role of physical exercise on Sestrin1 and 2 accumulations in the skeletal muscle of mice. Life Sciences 2017, 194: 98-103. PMID: 29273527, DOI: 10.1016/j.lfs.2017.12.023.Peer-Reviewed Original ResearchConceptsStress-related proteinsThreonine phosphorylationBXD miceSkeletal muscleSesn2 mRNATranscriptomic analysisProtein contentBioinformatics analysisIsogenic strainsSestrin 1SestrinsSESN1Phenotypic patternsSestrin1Sestrin2 expressionPhosphorylationMRNA levelsWestern blotProteinNew insightsExperimental approachBasal levelsLarge panelAccumulationMiceOverexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus
de Almeida Rodrigues B, Kuga G, Muñoz V, Gaspar R, Tavares M, Botezelli J, da Silva A, Cintra D, de Moura L, Simabuco F, Ropelle E, Pauli J. Overexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus. Neuroscience Letters 2017, 659: 14-17. PMID: 28866049, DOI: 10.1016/j.neulet.2017.08.067.Peer-Reviewed Original ResearchConceptsFoxO1 phosphorylationMKP-3 overexpressionDephosphorylation of FOXO1Potential therapeutic targetInjection of adenovirusOrexigenic neuropeptidesHypothalamic disorderHypothalamic third ventricleSwiss miceInsulin intracellularHypothalamic activationThird ventricleTherapeutic targetFoxO1 dephosphorylationAkt phosphorylationInsulin signalingImportant transcription factorDUSP6 geneHypothalamusSignificant reductionFOXO1MiceInsulin stimulationPresent studyInsulin signalPhysical exercise reduces pyruvate carboxylase (PCB) and contributes to hyperglycemia reduction in obese mice
Muñoz V, Gaspar R, Crisol B, Formigari G, Sant’Ana M, Botezelli J, Gaspar R, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Physical exercise reduces pyruvate carboxylase (PCB) and contributes to hyperglycemia reduction in obese mice. The Journal Of Physiological Sciences 2017, 68: 493-501. PMID: 28710665, PMCID: PMC10717866, DOI: 10.1007/s12576-017-0559-3.Peer-Reviewed Original ResearchConceptsDiet-induced obesityObesity-inducing dietInsulin tolerance testObese micePhysical exerciseDIO miceExercise trainingControl miceHepatic tissueStandard rodent chowDevelopment of hyperglycemiaExercise training protocolType 2 diabetesTreadmill running protocolPhysical exercise sessionGlucose homeostasis controlEXE miceHyperglycemia reductionObese conditionsRodent chowTolerance testSerum glucoseSwiss miceExercise sessionsEpididymal fat