2021
Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2016
Loss of Cystic Fibrosis Transmembrane Regulator Impairs Intestinal Oxalate Secretion
Knauf F, Thomson RB, Heneghan JF, Jiang Z, Adebamiro A, Thomson CL, Barone C, Asplin JR, Egan ME, Alper SL, Aronson PS. Loss of Cystic Fibrosis Transmembrane Regulator Impairs Intestinal Oxalate Secretion. Journal Of The American Society Of Nephrology 2016, 28: 242-249. PMID: 27313231, PMCID: PMC5198290, DOI: 10.1681/asn.2016030279.Peer-Reviewed Original ResearchConceptsIntestinal oxalate secretionWild-type miceCystic fibrosisIntestinal tissueOxalate secretionIncidence of hyperoxaluriaCalcium oxalate stone formationNet intestinal absorptionOxalate stone formationCoexpression of CFTRIntestinal transport processesWestern blot analysisOxalate absorptionMouse modelIntestinal absorptionGlucose absorptionUssing chambersStone formationFibrosisMiceSecretionReduced expressionCystic fibrosis transmembrane conductance regulator (CFTR) geneHyperoxaluriaPatients
1999
Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity
Whyte D, Li C, Thomson R, Nix S, Zanjani R, Karp S, Aronson P, Igarashi P. Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity. American Journal Of Physiology 1999, 277: f587-f598. PMID: 10516284, DOI: 10.1152/ajprenal.1999.277.4.f587.Peer-Reviewed Original ResearchConceptsHepatocyte nuclear factor 3Activator protein-2Renal epithelial cellsCAAT boxNuclear proteinsFlanking regionsPromoter activityTissue-specific gene expressionEpithelial cellsElectrophoretic mobility shift assaysKsp-cadherin gene promoterCCAAT/enhancer-binding proteinTranscription initiation siteKidney-specific memberMobility shift assaysConsensus promoter elementsEnhancer-binding proteinCell-specific activityPotential binding sitesLambda bacteriophage clonesLuciferase reporter plasmidHelix proteinsKidney epithelial cellsGATA factorsGC boxKsp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice
Igarashi P, Shashikant C, Thomson R, Whyte D, Liu-Chen S, Ruddle F, Aronson P. Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice. American Journal Of Physiology 1999, 277: f599-f610. PMID: 10516285, DOI: 10.1152/ajprenal.1999.277.4.f599.Peer-Reviewed Original ResearchConceptsKsp-cadherinEscherichia coli lacZ reporter geneTissue-specific memberKsp-cadherin gene promoterRepeat-induced geneTranscriptional start siteTissue-specific expressionDifferentiation-specific expressionBeta-galactosidase enzyme activityTransgene integration sitesKidney-specific expressionLacZ reporter geneTransgenic miceEpithelial cellsKidney-specific activityStart siteRegulatory elementsGene promoterReporter genePronuclear microinjectionKb upstreamUreteric budTubular epithelial cellsDevelopmental expressionIntegration sitesImmunolocalization of Ksp-cadherin in the adult and developing rabbit kidney
Thomson R, Aronson P. Immunolocalization of Ksp-cadherin in the adult and developing rabbit kidney. American Journal Of Physiology 1999, 277: f146-f156. PMID: 10409308, DOI: 10.1152/ajprenal.1999.277.1.f146.Peer-Reviewed Original Research
1995
Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *
Thomson R, Igarashi P, Biemesderfer D, Kim R, Abu-Alfa A, Soleimani M, Aronson P. Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *. Journal Of Biological Chemistry 1995, 270: 17594-17601. PMID: 7615566, DOI: 10.1074/jbc.270.29.17594.Peer-Reviewed Original ResearchConceptsKidney-specific memberMultigene familyKsp-cadherinCDNA cloningCharacteristic cysteine residuesT7 expression systemClassical cadherinsCytoplasmic domainCysteine residuesExpression systemTissue developmentImmunolocalization studiesRecognition sequenceNorthern analysisCadherinDemonstrated roleCellular recognitionMolecular analysisLI-cadherinHPT-1Basolateral membraneCloningRenal tubular epithelial cellsEpithelial cellsProtein