2019
A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System.
Ullah I, Chung K, Beloor J, Lee SK, Kumar P. A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System. Journal Of Visualized Experiments 2019 PMID: 31475960, DOI: 10.3791/59201.Peer-Reviewed Original ResearchConceptsCentral nervous systemBlood-brain barrierNervous systemIntranasal drug deliveryBody temperatureMin rest periodMouse body temperatureCNS uptakeReceptor-binding domainRabies virus glycoproteinCNS deliveryAnesthetized miceDominant handNondominant handMiceInhalationDelivery of siRNASiRNA approachDelivery of drugsRest periodHeating padMouse headAdministrationVirus glycoproteinForward position
2007
Transvascular delivery of small interfering RNA to the central nervous system
Kumar P, Wu H, McBride JL, Jung KE, Hee Kim M, Davidson BL, Kyung Lee S, Shankar P, Manjunath N. Transvascular delivery of small interfering RNA to the central nervous system. Nature 2007, 448: 39-43. PMID: 17572664, DOI: 10.1038/nature05901.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlood-Brain BarrierBrainCell LineDrug Delivery SystemsEncephalitis Virus, JapaneseEncephalitis, JapaneseGene SilencingGenetic VectorsGlycoproteinsGreen Fluorescent ProteinsHeLa CellsHumansLentivirusLiposomesMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred NODMice, SCIDMolecular Sequence DataNeuronsOligopeptidesRabies virusReceptors, NicotinicRecombinant ProteinsRNA, Small InterferingSuperoxide DismutaseSuperoxide Dismutase-1Viral ProteinsConceptsRVG-9RBlood-brain barrierRabies virus glycoproteinNeuronal cellsTransvascular deliveryFatal viral encephalitisCentral nervous systemTherapeutic moleculesIntravenous treatmentViral encephalitisInflammatory cytokinesAntiviral siRNAIntravenous injectionNervous systemAcetylcholine receptorsNeurological diseasesAnti-peptide antibodiesChimaeric peptidesNoninvasive approachDelivery of siRNABrainSiRNAMiceVirus glycoproteinRobust protection