2017
Effect of the Diabetic State on Islet Engraftment and Function in a Large Animal Model of Islet–Kidney Transplantation
Vallabhajosyula P, Hirakata A, Weiss M, Griesemer A, Shimizu A, Hong H, Habertheuer A, Tchipashvili V, Yamada K, Sachs DH. Effect of the Diabetic State on Islet Engraftment and Function in a Large Animal Model of Islet–Kidney Transplantation. Cell Transplantation 2017, 26: 1755-1762. PMID: 29338381, PMCID: PMC5784526, DOI: 10.1177/0963689717732993.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood GlucoseGlucose Tolerance TestHyperglycemiaIslets of Langerhans TransplantationKidney TransplantationPancreatectomySwineSwine, MiniatureConceptsDiabetic stateIslet transplantationLarge animal modelEngraftment periodIslet engraftmentControl donorsIslet equivalentsAnimal modelsLong-term islet functionSimilar glucose tolerance testsDonor animalsChronic diabetic stateEnd-organ injuryAutologous islet transplantationGlucose tolerance testMiniature swine modelNephrectomized recipientsTotal pancreatectomyDiabetic nephropathyRecipient weightGlycemic controlOrgan injuryTolerance inductionDonor weightIschemic factorsTissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue
Vallabhajosyula P, Korutla L, Habertheuer A, Yu M, Rostami S, Yuan CX, Reddy S, Liu C, Korutla V, Koeberlein B, Trofe-Clark J, Rickels MR, Naji A. Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue. Journal Of Clinical Investigation 2017, 127: 1375-1391. PMID: 28319051, PMCID: PMC5373894, DOI: 10.1172/jci87993.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersDiabetes Mellitus, Type 1ExosomesGraft RejectionHumansIslets of LangerhansIslets of Langerhans TransplantationKidney TransplantationMice, NudeMicroRNAsOrgan SpecificityProteomeConceptsRenal transplantationImmunologic rejectionTransplanted tissueClinical settingBiomarker platformAppearance of hyperglycemiaIslet transplant modelIslet β-cellsDonor exosomesTransplant isletsHLA antibodiesRecipient bloodTransplant modelRecipient circulationXenogeneic modelRecipient plasmaRenal epithelial cellsTransplant tissueHuman isletsΒ-cellsBiomarker potentialTransplantationNoninvasive windowEpithelial cellsExosomal microRNAs
2015
Monitoring of Allogeneic Islet Grafts in Nonhuman Primates Using MRI
Wang P, Schuetz C, Vallabhajosyula P, Medarova Z, Tena A, Wei L, Yamada K, Deng S, Markmann JF, Sachs DH, Moore A. Monitoring of Allogeneic Islet Grafts in Nonhuman Primates Using MRI. Transplantation 2015, 99: 1574-1581. PMID: 25806407, PMCID: PMC4551574, DOI: 10.1097/tp.0000000000000682.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAnimalsBiomarkersBlood GlucoseContrast MediaDextransDisease Models, AnimalGraft RejectionImmunosuppressive AgentsIslets of LangerhansIslets of Langerhans TransplantationMagnetic Resonance ImagingMagnetite NanoparticlesMaleOrgan SizePapio hamadryasPredictive Value of TestsStreptozocinTime FactorsTransplantation, HomologousConceptsGraft volumeIslet graftsBlood glucosePancreatic isletsNonhuman primatesAcute graft lossAllogeneic immune rejectionAllogeneic islet graftsWithdrawal of immunosuppressionPancreatic islet graftsVivo MRIGraft lossAllogeneic isletsGraft monitoringTreatment optionsIslet lossIslet transplantationRenal capsuleAllogeneic modelAutologous modelImmune rejectionMRISecondary damageImmunosuppressionIslets
2013
Assessing the Effect of Immunosuppression on Engraftment of Pancreatic Islets
Vallabhajosyula P, Hirakata A, Shimizu A, Okumi M, Tchipashvili V, Hong H, Yamada K, Sachs DH. Assessing the Effect of Immunosuppression on Engraftment of Pancreatic Islets. Transplantation 2013, 96: 372-378. PMID: 23883972, PMCID: PMC3786704, DOI: 10.1097/tp.0b013e31829f7515.Peer-Reviewed Original ResearchConceptsTriple-drug immunosuppressionIslet functionAllogeneic islet cell transplantationExperimental animalsLong-term islet functionTriple-drug immunosuppressive regimenIntravenous glucose tolerance testEquivalent glycemic controlEffect of immunosuppressionGlucose tolerance testNormal glucose regulationIslet cell transplantationVivo large animal modelLong-term functionIslet equivalents/Large animal modelImmunosuppressive regimenIslet doseImmunologic factorsRecipient weightEngrafted isletsGlycemic controlMinor histocompatibilityImmunosuppressive drugsCell transplantation
2011
Composite Islet-Kidneys From Single Baboon Donors Cure Diabetes Across Fully Allogenic Barriers
Yamada K, Hirakata A, Tchipashvili V, Shimizu A, Iwaki H, Griesemer A, Vallabhajosyula P, Moran S, Sachs DH. Composite Islet-Kidneys From Single Baboon Donors Cure Diabetes Across Fully Allogenic Barriers. American Journal Of Transplantation 2011, 11: 2603-2612. PMID: 21929644, PMCID: PMC3226882, DOI: 10.1111/j.1600-6143.2011.03733.x.Peer-Reviewed Original ResearchConceptsInsulin therapyFree isletsRenal capsuleEnd-stage diabetic nephropathyNonhuman primate modelPotential clinical applicabilityImmunosuppressive regimenAllogeneic barriersTotal pancreatectomyDiabetic nephropathyFBS levelsDiabetic hyperglycemiaPrimate modelPartial pancreatectomyDonor isletsMiniature swineNormal rangePotential cureGlucose regulationClinical applicabilityIsletsPancreatectomyTherapyIKsRecipients
2009
In Vivo Imaging of Autologous Islet Grafts in the Liver and Under the Kidney Capsule in Non-Human Primates
Medarova Z, Vallabhajosyula P, Tena A, Evgenov N, Pantazopoulos P, Tchipashvili V, Weir G, Sachs D, Moore A. In Vivo Imaging of Autologous Islet Grafts in the Liver and Under the Kidney Capsule in Non-Human Primates. Transplantation 2009, 87: 1659-1666. PMID: 19502957, PMCID: PMC2746764, DOI: 10.1097/tp.0b013e3181a5cbc0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisContrast MediaDextransFerrosoferric OxideInsulinInsulin SecretionIronIslets of Langerhans TransplantationKidneyLiver TransplantationMagnetic Resonance ImagingMagnetite NanoparticlesOxidesPancreatectomyPapioSubrenal Capsule AssayTransplantation, AutologousTransplantation, HeterologousConceptsMagnetic resonance imagingSuperparamagnetic iron oxide contrast agentIslet graftsIron oxide contrast agentNon-human primatesRenal subcapsular islet graftsNon-human primate modelIslet graft survivalVivo magnetic resonance imagingIntrahepatic graftsGraft survivalPosttransplantation periodKidney capsuleIslet transplantationPrimate modelRenal capsulePartial pancreatectomyLiver parenchymaContrast agentsDay 1Drug AdministrationIslet labelingClinical situationsHuman isletsResonance imaging
2007
Vascularized composite islet-kidney transplantation in a miniature swine model
Vallabhajosyula P, Griesemer A, Yamada K, Sachs DH. Vascularized composite islet-kidney transplantation in a miniature swine model. Cell Biochemistry And Biophysics 2007, 48: 201-207. PMID: 17709890, DOI: 10.1007/s12013-007-0027-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDiabetes Mellitus, Type 1Disease Models, AnimalGraft RejectionImmunosuppressive AgentsIslets of Langerhans TransplantationKidney TransplantationSwineSwine, MiniatureTreatment OutcomeConceptsThymic tissueIslet equivalentsEnd-stage diabetic nephropathyType I diabetic patientsAllogeneic thymic tissueRecipient body weightLong-term toleranceLong-term survivalMiniature swine modelKidney allograftsMHC barriersDiabetic nephropathyAllogeneic isletsAllogeneic transplantationDiabetic hyperglycemiaDiabetic patientsThymic componentsTreatment regimensPortal veinRenal capsuleOrgan transplantsAutologous isletsMiniature swineTransplantationBody weight