Featured Publications
Live imaging of SARS-CoV-2 infection in mice reveals that neutralizing antibodies require Fc function for optimal efficacy
Ullah I, Prévost J, Ladinsky MS, Stone H, Lu M, Anand SP, Beaudoin-Bussières G, Symmes K, Benlarbi M, Ding S, Gasser R, Fink C, Chen Y, Tauzin A, Goyette G, Bourassa C, Medjahed H, Mack M, Chung K, Wilen CB, Dekaban GA, Dikeakos JD, Bruce EA, Kaufmann DE, Stamatatos L, McGuire AT, Richard J, Pazgier M, Bjorkman PJ, Mothes W, Finzi A, Kumar P, Uchil PD. Live imaging of SARS-CoV-2 infection in mice reveals that neutralizing antibodies require Fc function for optimal efficacy. Immunity 2021, 54: 2143-2158.e15. PMID: 34453881, PMCID: PMC8372518, DOI: 10.1016/j.immuni.2021.08.015.Peer-Reviewed Original ResearchConceptsCOVID-19 convalescent subjectsSARS-CoV-2 infectionBioluminescence imagingK18-hACE2 miceLive bioluminescence imagingNatural killer cellsFc effector functionsSARS-CoV-2Convalescent subjectsKiller cellsPotent NAbsImmune protectionInflammatory responseEffector functionsNasal cavityNaB treatmentOptimal efficacyFc functionDepletion studiesMiceNAbsCOVID-19Direct neutralizationInfectionAntibodies
2019
Introducing Genes into Cultured Mammalian Cells
Kumar P, Nagarajan A, Uchil PD. Introducing Genes into Cultured Mammalian Cells. Cold Spring Harbor Protocols 2019, 2019: pdb.top095406. PMID: 31285274, DOI: 10.1101/pdb.top095406.Peer-Reviewed Original Research
2018
Analysis of Cell Viability by the alamarBlue Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the alamarBlue Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095489. PMID: 29858336, DOI: 10.1101/pdb.prot095489.Peer-Reviewed Original Research
2013
Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV
Zhong P, Agosto LM, Ilinskaya A, Dorjbal B, Truong R, Derse D, Uchil PD, Heidecker G, Mothes W. Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV. PLOS ONE 2013, 8: e53138. PMID: 23308151, PMCID: PMC3538641, DOI: 10.1371/journal.pone.0053138.Peer-Reviewed Original ResearchConceptsCell-free human immunodeficiency virusHuman immunodeficiency virusTarget cell typeCell transmissionTarget T cellsCell-free virusCell-free modeRestriction factor tetherinCell typesViral replication cycleHIV transmissionImmunodeficiency virusHIV cellT cellsTarget cellsDonor cellsVirus transmissionCell barrierPermissive cellsVirusProviral contentMultiple donorsReplication cycleCell contactExtracellular space
2003
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations
Kumar P, Uchil PD, Sulochana P, Nirmala G, Chandrashekar R, Haridattatreya M, Satchidanandam V. Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations. Archives Of Virology 2003, 148: 1569-1591. PMID: 12898332, DOI: 10.1007/s00705-003-0118-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ViralBaculoviridaeCells, CulturedCohort StudiesEncephalitis Virus, JapaneseEncephalitis, JapaneseEndemic DiseasesFemaleHumansImmunologic MemoryLymphocyte ActivationMaleRecombinant ProteinsRNA HelicasesSerine EndopeptidasesSpodopteraT-LymphocytesViral Nonstructural ProteinsViral ProteinsConceptsJapanese encephalitis virusCell-mediated immune responsesT cell responsesImmune responseJEV infectionT cellsCell responsesEncephalitis virusAdaptive T cell responsesHuman cohortsTh1 immune responseMemory T cellsCell preparationsImmune controlHealthy individualsCohortWhole cell preparationsViral proteinsInfectionNS3 proteinImportant targetVirusCritical determinantNS1NS5 protein