Featured Publications
TRIM5 is an innate immune sensor for the retrovirus capsid lattice
Pertel T, Hausmann S, Morger D, Züger S, Guerra J, Lascano J, Reinhard C, Santoni FA, Uchil PD, Chatel L, Bisiaux A, Albert ML, Strambio-De-Castillia C, Mothes W, Pizzato M, Grütter MG, Luban J. TRIM5 is an innate immune sensor for the retrovirus capsid lattice. Nature 2011, 472: 361-365. PMID: 21512573, PMCID: PMC3081621, DOI: 10.1038/nature09976.Peer-Reviewed Original ResearchMeSH KeywordsAntiviral Restriction FactorsCapsidCarrier ProteinsCell LineEnzyme ActivationHEK293 CellsHIV-1HumansImmunity, InnateLipopolysaccharidesMAP Kinase Kinase KinasesNF-kappa BProtein BindingReceptors, Pattern RecognitionRetroviridaeSignal TransductionTranscription Factor AP-1Transcription FactorsTripartite Motif ProteinsUbiquitinUbiquitin-Conjugating EnzymesUbiquitin-Protein Ligases
2019
Murine Leukemia Virus Exploits Innate Sensing by Toll-Like Receptor 7 in B-1 Cells To Establish Infection and Locally Spread in Mice
Pi R, Iwasaki A, Sewald X, Mothes W, Uchil PD. Murine Leukemia Virus Exploits Innate Sensing by Toll-Like Receptor 7 in B-1 Cells To Establish Infection and Locally Spread in Mice. Journal Of Virology 2019, 93: 10.1128/jvi.00930-19. PMID: 31434732, PMCID: PMC6803250, DOI: 10.1128/jvi.00930-19.Peer-Reviewed Original ResearchConceptsPopliteal lymph nodesFriend murine leukemia virusInnate immune sensing pathwaysToll-like receptor 7Viral spreadMurine leukemia virusCell-deficient miceType I interferon responseWild-type miceCell populationsType I interferonLeukemia virusRobust virus replicationI interferon responseAntiviral intervention strategiesInfected cell typesSentinel macrophagesAdoptive transferCell typesLymph nodesReceptor 7Virus infectionInnate sensingB cellsI interferon
2015
TRIM5 Retroviral Restriction Activity Correlates with the Ability To Induce Innate Immune Signaling
Lascano J, Uchil PD, Mothes W, Luban J. TRIM5 Retroviral Restriction Activity Correlates with the Ability To Induce Innate Immune Signaling. Journal Of Virology 2015, 90: 308-316. PMID: 26468522, PMCID: PMC4702541, DOI: 10.1128/jvi.02496-15.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCatsCell LineHumansImmunity, InnateMiceRetroviridaeSignal TransductionTranscription Factor AP-1Transduction, GeneticConceptsInnate immune signalingImmune signalingHIV-1 transductionHIV-1 restrictionAP-1 signalingViral nucleic acidsAP-1Retroviral capsid latticeAP-1 activationRestriction activityPast infectionProtein cyclophilin ARetrovirus infectionTRIM5Cyclophilin AActivity correlatesInfectionTRIM5 geneReverse transcriptionRetroviral transductionSignalingHost cell nucleusRetroviral capsidActivationTRIM family
2014
Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon Response
Pulloor NK, Nair S, McCaffrey K, Kostic AD, Bist P, Weaver JD, Riley AM, Tyagi R, Uchil PD, York JD, Snyder SH, García-Sastre A, Potter BV, Lin R, Shears SB, Xavier RJ, Krishnan MN. Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon Response. PLOS Pathogens 2014, 10: e1003981. PMID: 24586175, PMCID: PMC3937324, DOI: 10.1371/journal.ppat.1003981.Peer-Reviewed Original ResearchConceptsInositol pyrophosphatesHuman genome-wide RNAi screenGenome-wide RNAi screenNovel regulatory proteinPattern recognition receptor RIGActivation of IRF3RNAi screenPositive regulatorTranscription factorsType I interferon responseType I interferon productionNovel regulatorRegulatory proteinsKinase activityBiochemical experimentsPhosphoryl transferIntact cellsInnate immune responseMetabolic pathwaysInterferon transcriptionGlobal regulationIRF3 activationCellular infectionPPIP5K2PPIP5K1
2012
TRIM Protein-Mediated Regulation of Inflammatory and Innate Immune Signaling and Its Association with Antiretroviral Activity
Uchil PD, Hinz A, Siegel S, Coenen-Stass A, Pertel T, Luban J, Mothes W. TRIM Protein-Mediated Regulation of Inflammatory and Innate Immune Signaling and Its Association with Antiretroviral Activity. Journal Of Virology 2012, 87: 257-272. PMID: 23077300, PMCID: PMC3536418, DOI: 10.1128/jvi.01804-12.Peer-Reviewed Original ResearchConceptsInnate immune signalingNF-κBMurine leukemia virusImmune signalingInterferon productionAP-1Human immunodeficiency virus type 1 (HIV-1) gene expressionNF-κB/APHIV-1 resistanceVesicular stomatitis virus replicationTRIM proteinsLipopolysaccharide challengeAntiretroviral activityHIV-1NF-κB siteInnate immunityViral replicationVirus replicationPrimary macrophagesLeukemia virusCritical regulatorPathway upstreamSignalingKnockdownGene expression
2010
Anaplasma phagocytophilum AptA modulates Erk1/2 signalling
Sukumaran B, Mastronunzio JE, Narasimhan S, Fankhauser S, Uchil PD, Levy R, Graham M, Colpitts TM, Lesser CF, Fikrig E. Anaplasma phagocytophilum AptA modulates Erk1/2 signalling. Cellular Microbiology 2010, 13: 47-61. PMID: 20716207, PMCID: PMC3005019, DOI: 10.1111/j.1462-5822.2010.01516.x.Peer-Reviewed Original ResearchConceptsA. phagocytophilum infectionPhagocytophilum infectionCommon tick-borne diseasesHuman granulocytic anaplasmosisActivation of ERK1/2ERK1/2 mitogen-activated protein kinasesA. phagocytophilum survivalPolymorphonuclear leucocytesMitogen-activated protein kinaseHuman neutrophilsObligate intracellular pathogensGranulocytic anaplasmosisIntracellular pathogensTick-borne diseasesInfectionERK1/2 activationAnaplasma phagocytophilumVimentinSurvivalActivationBacterial inclusionsHost proteinsIntermediate filament protein vimentinVirulence proteinsProtein vimentin