2023
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.Peer-Reviewed Original ResearchConceptsC-terminal β-barrel domainSpike-mediated fusionCell-autonomous defenseLarge-scale exome sequencingΒ-barrel domainGenome-wide CRISPRSARS-CoV-2 infectionHost cell cytosolScramblase activityPhospholipid scramblaseLive SARS-CoV-2 infectionHuman lung epitheliumPLSCR1SARS-CoV-2 USASingle-molecule switchingSARS-CoV-2 variantsExome sequencingHuman populationRestriction factorsViral RNANew SARS-CoV-2 variantsSARS-CoV-2Robust activityLung epitheliumDefense factors
2006
Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells
Uchil P, Kumar A, Satchidanandam V. Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells. Journal Of Virology 2006, 80: 5451-5464. PMID: 16699025, PMCID: PMC1472159, DOI: 10.1128/jvi.01982-05.Peer-Reviewed Original ResearchConceptsReplicase proteinsReplication complexMembrane-bound replication complexesRNA synthesisViral replicase proteinsRNA-dependent RNA polymerase activityViral replication complexFlavivirus RNA synthesisCell nucleus functionsRNA polymerase activityReplicase complexHost cytoplasmNuclear matrixNuclear localizationNuclear locationPeripheral laminaIsolated nucleiImmunoelectron microscopyPolymerase activityJapanese encephalitis virusFlavivirus RNAInfected cellsProteinAbsolute requirementViral RNA