2024
Beta Spike-Presenting SARS-CoV-2 Virus-like Particle Vaccine Confers Broad Protection against Other VOCs in Mice
Ullah I, Symmes K, Keita K, Zhu L, Grunst M, Li W, Mothes W, Kumar P, Uchil P. Beta Spike-Presenting SARS-CoV-2 Virus-like Particle Vaccine Confers Broad Protection against Other VOCs in Mice. Vaccines 2024, 12: 1007. PMID: 39340037, PMCID: PMC11435481, DOI: 10.3390/vaccines12091007.Peer-Reviewed Original ResearchImmune responseVirus-like particlesBeta spikesCross-protective immune responsesSARS-CoV-2 immunitySARS-CoV-2Effective immune responseEffective humoral immune responseHumoral immune responseAncestral spikeT cellsVaccination regimenSARS-CoV-2 virus-like particlesVaccine platformMouse modelVariant spike proteinsOmicron spikeBeta variantDisease burdenNon-infectiousReduce virus spreadImmunityVariant spikesFusion glycoproteinSpike protein
2013
Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV
Zhong P, Agosto LM, Ilinskaya A, Dorjbal B, Truong R, Derse D, Uchil PD, Heidecker G, Mothes W. Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV. PLOS ONE 2013, 8: e53138. PMID: 23308151, PMCID: PMC3538641, DOI: 10.1371/journal.pone.0053138.Peer-Reviewed Original ResearchConceptsCell-free human immunodeficiency virusHuman immunodeficiency virusTarget cell typeCell transmissionTarget T cellsCell-free virusCell-free modeRestriction factor tetherinCell typesViral replication cycleHIV transmissionImmunodeficiency virusHIV cellT cellsTarget cellsDonor cellsVirus transmissionCell barrierPermissive cellsVirusProviral contentMultiple donorsReplication cycleCell contactExtracellular space
2011
TRIM22 Inhibits HIV-1 Transcription Independently of Its E3 Ubiquitin Ligase Activity, Tat, and NF-κB-Responsive Long Terminal Repeat Elements
Kajaste-Rudnitski A, Marelli SS, Pultrone C, Pertel T, Uchil PD, Mechti N, Mothes W, Poli G, Luban J, Vicenzi E. TRIM22 Inhibits HIV-1 Transcription Independently of Its E3 Ubiquitin Ligase Activity, Tat, and NF-κB-Responsive Long Terminal Repeat Elements. Journal Of Virology 2011, 85: 5183-5196. PMID: 21345949, PMCID: PMC3126207, DOI: 10.1128/jvi.02302-10.Peer-Reviewed Original ResearchConceptsHIV-1 replicationHIV-1 transcriptionHuman immunodeficiency virus type 1 (HIV-1) replicationU937 promonocytic cell lineHIV-1 restriction factorsTumor necrosis factor alphaInhibits HIV-1 transcriptionType 1 replicationRestriction factorsHIV-1 productionNecrosis factor alphaLower peak levelsPermissive cellsHIV-1 LTRHost restriction factorsPromonocytic cell lineU937 cellsReplication-competent virusNF-κB binding siteFactor alphaT cellsHIV-1Overexpression of TRIM22NF-κBLTR transactivation
2003
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations
Kumar P, Uchil PD, Sulochana P, Nirmala G, Chandrashekar R, Haridattatreya M, Satchidanandam V. Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations. Archives Of Virology 2003, 148: 1569-1591. PMID: 12898332, DOI: 10.1007/s00705-003-0118-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ViralBaculoviridaeCells, CulturedCohort StudiesEncephalitis Virus, JapaneseEncephalitis, JapaneseEndemic DiseasesFemaleHumansImmunologic MemoryLymphocyte ActivationMaleRecombinant ProteinsRNA HelicasesSerine EndopeptidasesSpodopteraT-LymphocytesViral Nonstructural ProteinsViral ProteinsConceptsJapanese encephalitis virusCell-mediated immune responsesT cell responsesImmune responseJEV infectionT cellsCell responsesEncephalitis virusAdaptive T cell responsesHuman cohortsTh1 immune responseMemory T cellsCell preparationsImmune controlHealthy individualsCohortWhole cell preparationsViral proteinsInfectionNS3 proteinImportant targetVirusCritical determinantNS1NS5 protein