2018
Optical Transfection.
Kumar P, Nagarajan A, Uchil PD. Optical Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096222. PMID: 30510131, DOI: 10.1101/pdb.top096222.Peer-Reviewed Original ResearchAnalysis of Cell Viability by the Lactate Dehydrogenase Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the Lactate Dehydrogenase Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095497. PMID: 29858337, DOI: 10.1101/pdb.prot095497.Peer-Reviewed Original ResearchConceptsCytoplasmic enzymePlasma membraneConversion of lactateLactate dehydrogenase assayLactate dehydrogenaseCellular damageYellow tetrazolium saltCell viabilityAmount of LDHDehydrogenase assayEnzymeCellsNADHTetrazolium saltCell culture supernatantsCulture supernatantsLDH activityAmount of formazanApoptosisDehydrogenaseMembraneActivityAssays
2008
Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides
Chan R, Uchil PD, Jin J, Shui G, Ott DE, Mothes W, Wenk MR. Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides. Journal Of Virology 2008, 82: 11228-11238. PMID: 18799574, PMCID: PMC2573248, DOI: 10.1128/jvi.00981-08.Peer-Reviewed Original ResearchConceptsPlasma membraneMurine leukemia virusHIV-1 buddingLipid contentLeukemia virusMass spectrometry analysisCholesterol-rich regionsOverall lipid contentCell peripheryMutant virionsLipid envelopeTotal lipid contentPhosphorylated derivativesSpectrometry analysisPhosphoinositideRetrovirusesMembraneCellsMatrix domainParticle releaseHuman immunodeficiency virus type 1Immunodeficiency virus type 1VirusVirus type 1PhosphatidylinositolMurine Leukemia Virus Spreading in Mice Impaired in the Biogenesis of Secretory Lysosomes and Ca2+-Regulated Exocytosis
Chan WT, Sherer NM, Uchil PD, Novak EK, Swank RT, Mothes W. Murine Leukemia Virus Spreading in Mice Impaired in the Biogenesis of Secretory Lysosomes and Ca2+-Regulated Exocytosis. PLOS ONE 2008, 3: e2713. PMID: 18629000, PMCID: PMC2443282, DOI: 10.1371/journal.pone.0002713.Peer-Reviewed Original ResearchConceptsMurine leukemia virusMultivesicular bodiesPlasma membraneHermansky-Pudlak syndromeLysosome related organellesFunctional AP-3Moloney murine leukemia virusMVB pathwaySynaptotagmin VIILeukemia virusRelated organellesLysosome fusionAP-3Secretory lysosomesMost mutant miceMLV replicationWild-type miceCellular levelBiogenesisModerate reductionEssential roleVirus releaseRelease pathwayMutant miceImmunological alterations