2019
Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized mice
Ventura JD, Beloor J, Allen E, Zhang T, Haugh KA, Uchil PD, Ochsenbauer C, Kieffer C, Kumar P, Hope TJ, Mothes W. Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized mice. PLOS Pathogens 2019, 15: e1008161. PMID: 31805155, PMCID: PMC6917343, DOI: 10.1371/journal.ppat.1008161.Peer-Reviewed Original ResearchConceptsHIV-1 infectionHumanized miceCombination antiretroviral therapy regimenViral spreadHIV-1 infection dynamicsNon-invasive bioluminescentAntiretroviral therapy regimenHIV-1 reporterSame lymphoid tissuesInfected cell populationCART withdrawalInfection recrudescenceAntiretroviral therapyTreatment interruptionTherapy regimenLymphoid tissueInfection dynamicsART treatmentBioluminescent imagingInfectionViral infection dynamicsInfected cellsCell populationsMiceBioluminescent signal
2006
Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells
Uchil P, Kumar A, Satchidanandam V. Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells. Journal Of Virology 2006, 80: 5451-5464. PMID: 16699025, PMCID: PMC1472159, DOI: 10.1128/jvi.01982-05.Peer-Reviewed Original ResearchConceptsReplicase proteinsReplication complexMembrane-bound replication complexesRNA synthesisViral replicase proteinsRNA-dependent RNA polymerase activityViral replication complexFlavivirus RNA synthesisCell nucleus functionsRNA polymerase activityReplicase complexHost cytoplasmNuclear matrixNuclear localizationNuclear locationPeripheral laminaIsolated nucleiImmunoelectron microscopyPolymerase activityJapanese encephalitis virusFlavivirus RNAInfected cellsProteinAbsolute requirementViral RNA