2018
Analysis of Cell Viability by the MTT Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095505. PMID: 29858338, DOI: 10.1101/pdb.prot095505.Peer-Reviewed Original ResearchConceptsInsoluble purple formazanMitochondrial reductaseCell stressCell deathLive cellsDirect cell deathConversion of substrateMetabolic activityCell viabilityPurple formazanViability assaysNonadherent cellsPlate formatCellsAssaysMTT assayMTTChromogenic productSensitive assayReductaseToxic agentsPopular assayFormazanViabilityLarge changes
2015
HIV cell-to-cell transmission: effects on pathogenesis and antiretroviral therapy
Agosto LM, Uchil PD, Mothes W. HIV cell-to-cell transmission: effects on pathogenesis and antiretroviral therapy. Trends In Microbiology 2015, 23: 289-295. PMID: 25766144, PMCID: PMC4417442, DOI: 10.1016/j.tim.2015.02.003.Peer-Reviewed Original ResearchConceptsAntiretroviral therapyVirological synapsesT-cell depletionCell transmissionHIV infectionCell depletionHIV cellMatter of debateViral replicationTherapyUninfected cellsReduced efficacyHigh MOISynapsesHIVPathogenesisRecent dataInfectionCell deathTrigger cell deathCellsVivoCell-cell contactCellular surveillanceHigher number