Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells. Elevations in tetrahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity.
Rosenkranz-Weiss P, Sessa WC, Milstien S, Kaufman S, Watson CA, Pober JS. Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells. Elevations in tetrahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity. Journal Of Clinical Investigation 1994, 93: 2236-2243. PMID: 7514193, PMCID: PMC294374, DOI: 10.1172/jci117221.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsNitric oxide synthaseIL-1 betaNOS activityTetrahydrobiopterin levelsUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsIFN-gammaCytokine treatmentEndothelial nitric oxide synthaseTotal NOS activityNitric oxide synthesisCultured human endothelial cellsCytokine-treated cellsNitric oxide releaseGTP cyclohydrolase I activityHuman endothelial cellsProinflammatory cytokinesInflammatory cytokinesIntracellular tetrahydrobiopterin levelsOxide synthaseCytokine regulationRate-limiting enzymeOxide synthesis