2023
Approaches to pandemic prevention – the chromatin vaccine
Zhang J, Askenase P, Jaenisch R, Crumpacker C. Approaches to pandemic prevention – the chromatin vaccine. Frontiers In Immunology 2023, 14: 1324084. PMID: 38143744, PMCID: PMC10739501, DOI: 10.3389/fimmu.2023.1324084.Peer-Reviewed Original ResearchConceptsEffective vaccineViral infectionCoronavirus diseaseImmunodeficiency syndromeHIV DNASuccessful vaccinePolio vaccineHIV integrationVaccineNext pandemicViral replicationIneffective vaccinesInfectionEpisomal formFuture pandemicsCurrent pandemicPandemic preventionPreventionPandemicEnd productsEpigenetic silencingSyndromeDiseaseHuman population
2022
Systems Vaccinology in HIV Vaccine Development
Zhang J, Askenase P, Crumpacker C. Systems Vaccinology in HIV Vaccine Development. Vaccines 2022, 10: 1624. PMID: 36298489, PMCID: PMC9611490, DOI: 10.3390/vaccines10101624.Peer-Reviewed Original ResearchExosome Carrier Effects; Resistance to Digestion in Phagolysosomes May Assist Transfers to Targeted Cells; II Transfers of miRNAs Are Better Analyzed via Systems Approach as They Do Not Fit Conventional Reductionist Stoichiometric Concepts
Askenase PW. Exosome Carrier Effects; Resistance to Digestion in Phagolysosomes May Assist Transfers to Targeted Cells; II Transfers of miRNAs Are Better Analyzed via Systems Approach as They Do Not Fit Conventional Reductionist Stoichiometric Concepts. International Journal Of Molecular Sciences 2022, 23: 6192. PMID: 35682875, PMCID: PMC9181154, DOI: 10.3390/ijms23116192.Peer-Reviewed Original Research
2021
Rare Skin Reactions after mRNA Vaccination, Similar to Jones–Mote Basophil Responses
Askenase PW. Rare Skin Reactions after mRNA Vaccination, Similar to Jones–Mote Basophil Responses. New England Journal Of Medicine 2021, 385: 1720-1721. PMID: 34706177, PMCID: PMC8609603, DOI: 10.1056/nejmc2111452.Peer-Reviewed Original ResearchSmall extracellular vesicles released by infused mesenchymal stromal cells target M2 macrophages and promote TGF‐β upregulation, microvascular stabilization and functional recovery in a rodent model of severe spinal cord injury
Nakazaki M, Morita T, Lankford KL, Askenase PW, Kocsis JD. Small extracellular vesicles released by infused mesenchymal stromal cells target M2 macrophages and promote TGF‐β upregulation, microvascular stabilization and functional recovery in a rodent model of severe spinal cord injury. Journal Of Extracellular Vesicles 2021, 10: e12137. PMID: 34478241, PMCID: PMC8408371, DOI: 10.1002/jev2.12137.Peer-Reviewed Original ResearchConceptsMarrow-derived mesenchymal stem/stromal cellsSpinal cord injuryBlood-spinal cord barrierSmall extracellular vesiclesFunctional recoveryM2 macrophagesCord injuryInjury siteTherapeutic effectStromal cellsSevere spinal cord injurySingle MSC injectionImproved functional recoveryBone marrow-derived mesenchymal stem/stromal cellsM2 macrophage markersSimilar therapeutic effectsRelease of sEVsMesenchymal stem/stromal cellsExtracellular vesiclesTight junction proteinsStem/stromal cellsMesenchymal stromal cellsTGF-β receptorMSC infusionSCI ratsAntibodies Enhance the Suppressive Activity of Extracellular Vesicles in Mouse Delayed-Type Hypersensitivity
Nazimek K, Bustos-Morán E, Blas-Rus N, Nowak B, Totoń-Żurańska J, Seweryn MT, Wołkow P, Woźnicka O, Szatanek R, Siedlar M, Askenase PW, Sánchez-Madrid F, Bryniarski K. Antibodies Enhance the Suppressive Activity of Extracellular Vesicles in Mouse Delayed-Type Hypersensitivity. Pharmaceuticals 2021, 14: 734. PMID: 34451831, PMCID: PMC8398949, DOI: 10.3390/ph14080734.Peer-Reviewed Original ResearchDelayed-type hypersensitivityMice delayed-type hypersensitivityAntigen-specific antibodiesEffector T cellsT cellsSuppressive activityExtracellular vesiclesAntigen-presenting macrophagesMHC class IIGreat therapeutic potentialMouse modelSuppressive actionCurrent studyMiRNA-150Therapeutic potentialClass IIFlow cytometryExact mechanismAntibodiesParental cellsTherapeutic usageHypersensitivityMacrophagesIntercellular communicationSpecific bindingExosomes provide unappreciated carrier effects that assist transfers of their miRNAs to targeted cells; I. They are ‘The Elephant in the Room’
Askenase PW. Exosomes provide unappreciated carrier effects that assist transfers of their miRNAs to targeted cells; I. They are ‘The Elephant in the Room’. RNA Biology 2021, 18: 2038-2053. PMID: 33944671, PMCID: PMC8582996, DOI: 10.1080/15476286.2021.1885189.Peer-Reviewed Original ResearchConceptsExtracellular vesiclesAcceptor cellsOuter membrane vesiclesMiRNA transferMolecular chemical changesNano-extracellular vesiclesCargo moleculesExosome transferMembrane vesiclesMiRNAsGenetic expressionDNA expressionExosomesVesiclesCell functionIntracellular effectsFunctional effectsFunctional alterationsMolecular transferCellsPhysiologic processesLight chainExpressionMitochondriaBiology
2013
Antigen-specific, antibody-coated, exosome-like nanovesicles deliver suppressor T-cell microRNA-150 to effector T cells to inhibit contact sensitivity
Bryniarski K, Ptak W, Jayakumar A, Püllmann K, Caplan MJ, Chairoungdua A, Lu J, Adams BD, Sikora E, Nazimek K, Marquez S, Kleinstein SH, Sangwung P, Iwakiri Y, Delgato E, Redegeld F, Blokhuis BR, Wojcikowski J, Daniel AW, Kormelink T, Askenase PW. Antigen-specific, antibody-coated, exosome-like nanovesicles deliver suppressor T-cell microRNA-150 to effector T cells to inhibit contact sensitivity. Journal Of Allergy And Clinical Immunology 2013, 132: 170-181.e9. PMID: 23727037, PMCID: PMC4176620, DOI: 10.1016/j.jaci.2013.04.048.Peer-Reviewed Original ResearchConceptsCutaneous contact sensitivityEffector T cellsT cell toleranceT cellsExosome-like nanovesiclesContact sensitivityCS-effector T cellsMiRNA-150Regulatory T cellsAntigen-specific mannerSuppressor T cellsRole of antibodiesAdoptive cell transfer modelCell transfer modelT cell regulationLight chainSuppressor cellsLymph nodesReactive haptenImmune suppressionMicroRNA-150Systemic injectionAntibody light chainIntravenous injectionSpleen cells
2011
Stimulatory Lipids Accumulate in the Mouse Liver within 30 min of Contact Sensitization to Facilitate the Activation of Naïve iNKT Cells in a CD1d‐Dependent Fashion
Dey N, Szczepanik M, Lau K, Majewska‐Szczepanik M, Askenase PW. Stimulatory Lipids Accumulate in the Mouse Liver within 30 min of Contact Sensitization to Facilitate the Activation of Naïve iNKT Cells in a CD1d‐Dependent Fashion. Scandinavian Journal Of Immunology 2011, 74: 52-61. PMID: 21352253, DOI: 10.1111/j.1365-3083.2011.02540.x.Peer-Reviewed Original ResearchConceptsHepatic iNKT cellsINKT cellsContact sensitivityHepatic lipidsT cellsB cellsNatural killer T cellsAdoptive transfer techniquesEffector T cellsLiver mononuclear cellsCutaneous inflammatory responseINKT cell activationKiller T cellsMouse liverΑβ T cell receptorAllergic contact dermatitisInnate immune systemRecipient mouse liversSame allergenContact hypersensitivityTopical sensitizationContact sensitizationIgM antibodiesMononuclear cellsIL-4Participation of Inkt Cells in the Early and Late Components of Tc1‐Mediated DNFB Contact Sensitivity: Cooperative Role of γδ‐T Cells
Askenase PW, Majewska‐Szczepanik M, Kerfoot S, Szczepanik M. Participation of Inkt Cells in the Early and Late Components of Tc1‐Mediated DNFB Contact Sensitivity: Cooperative Role of γδ‐T Cells. Scandinavian Journal Of Immunology 2011, 73: 465-477. PMID: 21272050, DOI: 10.1111/j.1365-3083.2011.02522.x.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsDermatitis, ContactDinitrofluorobenzeneFemaleFlow CytometryImmunizationImmunophenotypingInterferon-gammaMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred CBAMice, KnockoutNatural Killer T-CellsReceptors, Antigen, T-Cell, alpha-betaSpecific Pathogen-Free OrganismsT-Lymphocytes, Cytotoxic
2009
Epicutaneous Immunization with Protein Antigen in the Presence of TLR4 Ligand Induces TCRαβ+CD4+ T Contrasuppressor Cells That Reverse Skin-Induced Suppression of Th1-Mediated Contact Sensitivity
Ptak W, Majewska M, Bryniarski K, Ptak M, Lobo FM, Zając K, Askenase PW, Szczepanik M. Epicutaneous Immunization with Protein Antigen in the Presence of TLR4 Ligand Induces TCRαβ+CD4+ T Contrasuppressor Cells That Reverse Skin-Induced Suppression of Th1-Mediated Contact Sensitivity. The Journal Of Immunology 2009, 182: 837-850. PMID: 19124727, DOI: 10.4049/jimmunol.182.2.837.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, CutaneousAnimalsCD4-Positive T-LymphocytesCells, CulturedContraindicationsDermatitis, ContactImmune ToleranceImmunizationLigandsLipopolysaccharidesMaleMiceMice, Inbred BALB CMice, Inbred CBAMice, KnockoutMice, TransgenicPatch TestsReceptors, Antigen, T-Cell, alpha-betaSkinTh1 CellsToll-Like Receptor 4TrinitrobenzenesConceptsEpicutaneous immunizationEffector cellsIL-12Effector phaseT cellsIFN-gammaTC cellsContrasuppressor T cellsCS effector cellsIL-12 neutralizationSkin-induced suppressionContact sensitivity reactionTh1 effector cellsTLR4 ligand LPSMechanism of actionEC immunizationSuppressor CD4Th1 CD4Regulatory cellsRIL-12TLR4 ligandIL-6Contact sensitivityLigand LPSINF-gammaNK cell mediated contact sensitivity is elicitable 1 hour after immunization and depends on IFN-? and IL-12 production
Askenase PW, Majewska M, and Szczepanik M. NK cell mediated contact sensitivity is elicitable 1 hour after immunization and depends on IFN-? and IL-12 production. Submitted 2009.Peer-Reviewed Original ResearchRole of B-1 cells in early acquired protection from pneumococcal pneumonia: Immune B-1 cells reconstitute defective protection on AID-/- mice
Yamamoto N, Kerfoot S, and Askenase PW. Role of B-1 cells in early acquired protection from pneumococcal pneumonia: Immune B-1 cells reconstitute defective protection on AID-/- mice. Submitted 2009Peer-Reviewed Original Research
2008
Identification of Initiator B Cells, a Novel Subset of Activation-Induced Deaminase-Dependent B-1-Like Cells That Mediate Initiation of Contact Sensitivity
Kerfoot SM, Szczepanik M, Tung JW, Askenase PW. Identification of Initiator B Cells, a Novel Subset of Activation-Induced Deaminase-Dependent B-1-Like Cells That Mediate Initiation of Contact Sensitivity. The Journal Of Immunology 2008, 181: 1717-1727. PMID: 18641308, DOI: 10.4049/jimmunol.181.3.1717.Peer-Reviewed Original ResearchConceptsContact sensitivityB cellsCS initiationInflammatory responseT cell-mediated inflammationProinflammatory T cellsCell-mediated inflammationAdoptive transfer experimentsDelayed-type hypersensitivityAg-specific IgMReceptor gene mutationsSensitized miceVascular activationDeficient recipientsT cellsNovel subsetSomatic hypermutationNovel subpopulationGene mutationsIgMMiceActivation-Induced DeaminaseWT cellsCellsChain gene
2007
c-Jun NH2-Terminal Kinase 2 Inhibits Gamma Interferon Production during Anaplasma phagocytophilum Infection
Pedra JH, Mattner J, Tao J, Kerfoot SM, Davis RJ, Flavell RA, Askenase PW, Yin Z, Fikrig E. c-Jun NH2-Terminal Kinase 2 Inhibits Gamma Interferon Production during Anaplasma phagocytophilum Infection. Infection And Immunity 2007, 76: 308-316. PMID: 17998313, PMCID: PMC2223674, DOI: 10.1128/iai.00599-07.Peer-Reviewed Original ResearchConceptsIFN-gamma productionA. phagocytophilum infectionPhagocytophilum infectionIFN-gammaJnk2-null miceNatural killer T cellsA. phagocytophilumKiller T cellsIFN-gamma releaseIFN-gamma secretionGamma interferon productionT cell agonistsAnaplasma phagocytophilum infectionT cellsEarly eradicationGamma interferonInterferon productionInfectionC-Jun NH2-terminal kinase-2Inhibitory effectElevated levelsMiceAnaplasma phagocytophilumPhagocytophilumKinase 2
2006
Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells
Itakura A, Kikuchi Y, Kouro T, Ikutani M, Takaki S, Askenase PW, Takatsu K. Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells. International Archives Of Allergy And Immunology 2006, 140: 8-16. PMID: 16772721, DOI: 10.1159/000092705.Peer-Reviewed Original ResearchConceptsElicitation of CSIgM-producing cellsContact sensitivityEosinophil peroxidase activityEosinophil infiltrationIL-5Antigen-specific immunoglobulin M antibodyCS-effector T cellsEnzyme-linked immunospot assayLocal tissueEffector T cellsRole of interleukinImmunoglobulin M antibodiesIL-5 receptorImmunized ILImmunospot assayContact sensitizationM antibodiesMononuclear cellsDeficient micePolymorphonuclear cellsT cellsImmune responseCell activationInterleukin-5
1999
C-C chemokines in allergen-induced late-phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils, and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia, and relationship to basophils and other C-C chemokines (monocyte chemoattractant protein-3 and RANTES).
Ying S, Robinson D, Meng Q, Barata L, McEuen A, Buckley M, Walls A, Askenase P, Kay A. C-C chemokines in allergen-induced late-phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils, and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia, and relationship to basophils and other C-C chemokines (monocyte chemoattractant protein-3 and RANTES). The Journal Of Immunology 1999, 163: 3976-84. PMID: 10491000, DOI: 10.4049/jimmunol.163.7.3976.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAllergensBasophilsCell MovementChemokine CCL11Chemokine CCL24Chemokine CCL5Chemokine CCL7Chemokines, CCCytokinesDermatitis, AtopicEosinophiliaEosinophilsHumansImmunophenotypingMiddle AgedMonocyte Chemoattractant ProteinsReceptors, CCR3Receptors, ChemokineRNA, MessengerTime FactorsConceptsMonocyte chemoattractant protein-3C-C chemokinesEotaxin-2MCP-4T cellsAtopic subjectsLate-phase cutaneous responseCD4+ T cellsEotaxin receptor CCR3Human atopic subjectsMonocyte chemoattractant protein-4CD3+ cellsCD68+ macrophagesEG2+ eosinophilsExpression of eotaxinCCR3+ cellsMCP-4 mRNAElastase+ neutrophilsLate eosinophiliaAllergic tissue reactionsEotaxin proteinCD3+Allergen-inducedRelationship of expressionEotaxin mRNA
1996
Delayed-type hypersensitivity in mast cell-deficient mice: dependence on platelets for expression of contact sensitivity.
Geba GP, Ptak W, Anderson GM, Paliwal V, Ratzlaff RE, Levin J, Askenase PW. Delayed-type hypersensitivity in mast cell-deficient mice: dependence on platelets for expression of contact sensitivity. The Journal Of Immunology 1996, 157: 557-65. PMID: 8752902, DOI: 10.4049/jimmunol.157.2.557.Peer-Reviewed Original ResearchConceptsMast cell-deficient miceCell-deficient miceDelayed-type hypersensitivityContact sensitivityPlatelet depletionMast cellsCS responsesT cell-mediated immune responsesMast cell-deficient strainsDelayed-type hypersensitivity responseT cell-mediated responsesCell-mediated immune responsesTissue levelsCutaneous sensitivity responsesVasoactive amine serotoninCell-mediated responsesLocal mast cellsMast cell numbersNormal mast cell numbersHypersensitivity responseCutaneous responseImmunologic evidenceBeige miceImmune responseAmine serotonin
1992
Molecular regulation of tumor necrosis factor-alpha and lymphotoxin production in T cells. Inhibition by prostaglandin E2.
Ferreri NR, Sarr T, Askenase PW, Ruddle NH. Molecular regulation of tumor necrosis factor-alpha and lymphotoxin production in T cells. Inhibition by prostaglandin E2. Journal Of Biological Chemistry 1992, 267: 9443-9449. PMID: 1533632, DOI: 10.1016/s0021-9258(19)50443-4.Peer-Reviewed Original ResearchConceptsTNF-alphaProstaglandin E2T cell clonesT cellsTumor necrosisLymphotoxin productionLT productionMajor histocompatibility complex class IIHistocompatibility complex class IICell clonesPresence of PGE2M prostaglandin E2Dose-dependent mannerPGF2 alphaSoluble concanavalin ALeukotriene C4Murine Th1Inhibited productionClass IIFibrosarcoma cell lineInhibitory effectMurine fibrosarcoma cell lineBiological activityConcanavalin ACell linesRole of Gamma/Delta T Cells as Positive Regulators of Alpha/Beta TCR+ Effector T Cells in Allergic Contact Sensitivity
Askenase PW, Ptak W. Role of Gamma/Delta T Cells as Positive Regulators of Alpha/Beta TCR+ Effector T Cells in Allergic Contact Sensitivity. International Archives Of Allergy And Immunology 1992, 99: 246-251. PMID: 34167199, DOI: 10.1159/000236258.Peer-Reviewed Original ResearchCutaneous contact sensitivityEffector T cellsCS-effector T cellsT cell receptorΓδ T cellsT cellsCS responsesContact sensitivityGamma/delta T cellsCell transferCS effector cellsDelta T cellsMinor histocompatibility antigensAdoptive cell transferΑβ T cellsAllergic contact sensitivityTCR mAbEffector cellsΓδ TΑβ TAlpha/betaRabbit complementHistocompatibility antigensVitro treatmentCell receptor