2022
SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to down-regulate MHC-I surface expression
Arshad N, Laurent-Rolle M, Ahmed W, Hsu J, Mitchell S, Pawlak J, Sengupta D, Biswas K, Cresswell P. SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to down-regulate MHC-I surface expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 120: e2208525120. PMID: 36574644, PMCID: PMC9910621, DOI: 10.1073/pnas.2208525120.Peer-Reviewed Original ResearchConceptsMHC-I expressionSARS-CoV-2Major histocompatibility complex (MHC) class I moleculesT cell recognitionVirus-infected cellsClass I moleculesAntigen presentationOngoing COVID-19 pandemicHeavy chainImmune evasionViral peptidesSecretory pathwayDistinct mechanismsMHCI moleculesPeptide-MHCInfected cellsCausative agentCell recognitionCD8COVID-19 pandemicViral proteinsEndoplasmic reticulumHuman MHCORF7a
1998
Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors.
Deng Y, Gibbs J, Bačík I, Porgador A, Copeman J, Lehner P, Ortmann B, Cresswell P, Bennink J, Yewdell J. Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors. The Journal Of Immunology 1998, 161: 1677-85. PMID: 9712031, DOI: 10.4049/jimmunol.161.4.1677.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntibodies, MonoclonalAntiportersCell LineEndoplasmic ReticulumH-2 AntigensHeLa CellsHumansImmunoglobulinsLymphocyte ActivationMacromolecular SubstancesMembrane Transport ProteinsMiceOligopeptidesOvalbuminPeptide FragmentsProtease InhibitorsRecombinant ProteinsT-LymphocytesVaccinia virusConceptsInsect cellsEndoplasmic reticulumVertebrate cellsHuman cellsHuman tapasinVaccinia virus-mediated expressionCell surface expressionProtease inhibitorsInefficient assemblyKbMHC class IMouse betaInsectsEfficient assemblyImmediate precursorSurface expressionAntigenic peptidesHeavy chainClass IRecombinant vaccinia virusVirus-mediated expressionAssemblyExpressionCellsVaccinia virus
1997
Misfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome
Hughes E, Hammond C, Cresswell P. Misfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1896-1901. PMID: 9050876, PMCID: PMC20014, DOI: 10.1073/pnas.94.5.1896.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcysteineBeta 2-MicroglobulinBlotting, WesternCysteine EndopeptidasesCysteine Proteinase InhibitorsCytoplasmEndoplasmic ReticulumEnzyme InhibitorsGlycosylationHistocompatibility Antigens Class IHumansKineticsLeupeptinsMultienzyme ComplexesProteasome Endopeptidase ComplexProtein FoldingSolubilityTransfectionTumor Cells, CulturedConceptsClass I heavy chainsMHC class I heavy chainMajor histocompatibility complex class I heavy chainsBeta2-microglobulinHeavy chainMHC class ICell linesCell line DaudiTAP-deficient cell linesSpecific irreversible inhibitorClass IHerpes simplex virus proteinDaudiVirus proteinsEndoplasmic reticulumIrreversible inhibitorSimilar accumulationLactacystinPeriod of hours
1987
An epitope common to HLA class I and class II antigens, Ig light chains, and β2-microglobulin
Lutz P, Cresswell P. An epitope common to HLA class I and class II antigens, Ig light chains, and β2-microglobulin. Immunogenetics 1987, 25: 228-233. PMID: 2437022, DOI: 10.1007/bf00404692.Peer-Reviewed Original ResearchConceptsB-lymphoblastoid cell linesClass I heavy chainsWestern blottingClass I major histocompatibility complex antigensClass II MHC antigensMajor histocompatibility complex antigensClass IHLA class I heavy chainsClass II antigensHistocompatibility complex antigensHLA class IBeta-2-microglobulinLight chainHeavy chainNormal human serumWestern blot analysisMHC antigensII antigensComplex antigensImmunoglobulin light chainsM1 mAbΒ2-microglobulinMonoclonal antibodiesAntigenHuman immunoglobulin
1984
Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids
Howell D, Kostyu D, Ting J, Cresswell P. Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids. Somatic Cell And Molecular Genetics 1984, 10: 217-224. PMID: 6609440, DOI: 10.1007/bf01535244.Peer-Reviewed Original ResearchConceptsClass I histocompatibilityB-LCLLymphoblastoid cell linesB antigensPaucity of HLAHLA class I heavy chainsHLA-B antigensHLA-A antigensB-lymphoblastoid cell linesB antigen expressionClass I heavy chainsAntigen expressionB expressionHLAIndirect immunofluorescenceAntigenLow levelsClass IT-LCLHistocompatibilityCell linesHeavy chain mRNATotal levelsCEMRHeavy chain