1998
Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors.
Deng Y, Gibbs J, Bačík I, Porgador A, Copeman J, Lehner P, Ortmann B, Cresswell P, Bennink J, Yewdell J. Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors. The Journal Of Immunology 1998, 161: 1677-85. PMID: 9712031, DOI: 10.4049/jimmunol.161.4.1677.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntibodies, MonoclonalAntiportersCell LineEndoplasmic ReticulumH-2 AntigensHeLa CellsHumansImmunoglobulinsLymphocyte ActivationMacromolecular SubstancesMembrane Transport ProteinsMiceOligopeptidesOvalbuminPeptide FragmentsProtease InhibitorsRecombinant ProteinsT-LymphocytesVaccinia virusConceptsInsect cellsEndoplasmic reticulumVertebrate cellsHuman cellsHuman tapasinVaccinia virus-mediated expressionCell surface expressionProtease inhibitorsInefficient assemblyKbMHC class IMouse betaInsectsEfficient assemblyImmediate precursorSurface expressionAntigenic peptidesHeavy chainClass IRecombinant vaccinia virusVirus-mediated expressionAssemblyExpressionCellsVaccinia virus
1987
Regulation of HLA Class I and Class II antigen expression
Cresswell P. Regulation of HLA Class I and Class II antigen expression. British Medical Bulletin 1987, 43: 66-80. PMID: 3315102, DOI: 10.1093/oxfordjournals.bmb.a072177.Peer-Reviewed Original ResearchConceptsCell surfaceRegulatory genesTranscriptional levelHybridisation experimentsIntracellular transportThird glycoproteinHeterodimeric glycoproteinCell typesChondroitin sulfate proteoglycanEfficient assemblyCell linesSulfate proteoglycanEnhanced expressionT cell linesGenesClass IExpressionTissue distributionRegulationLymphoblastoid linesGlycoproteinUnusual complexityClass I moleculesInvariant chainBiosynthesis