1998
Elucidation of the genetic basis of the antigen presentation defects in the mutant cell line .220 reveals polymorphism and alternative splicing of the tapasin gene
Copeman J, Bangia N, Cross J, Cresswell P. Elucidation of the genetic basis of the antigen presentation defects in the mutant cell line .220 reveals polymorphism and alternative splicing of the tapasin gene. European Journal Of Immunology 1998, 28: 3783-3791. PMID: 9842921, DOI: 10.1002/(sici)1521-4141(199811)28:11<3783::aid-immu3783>3.0.co;2-9.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAntigen PresentationAntiportersATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersB-LymphocytesCell LineDNA, ComplementaryEndoplasmic ReticulumExonsHumansImmunoglobulinsMembrane Transport ProteinsMutationPolymorphism, GeneticReverse Transcriptase Polymerase Chain ReactionConceptsMutant cell linesEndoplasmic reticulumAlternative splicingN-terminal 49 amino acidsGenetic basisTapasin geneExon twoWild-type cellsFull-length transcriptsCell linesSingle nucleotide substitutionSignal peptideSecond intronNucleotide substitutionsPhysical associationSplice siteGlycoprotein tapasinPosition 240Amino acidsClass I moleculesSplicingOptimal bindingGenesI moleculesHeterodimersInduction of transporter associated with antigen processing by interferon γ confers endothelial cell cytoprotection against natural killer-mediated lysis
Ayalon O, Hughes E, Cresswell P, Lee J, O’Donnell L, Pardi R, Bender J. Induction of transporter associated with antigen processing by interferon γ confers endothelial cell cytoprotection against natural killer-mediated lysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2435-2440. PMID: 9482903, PMCID: PMC19366, DOI: 10.1073/pnas.95.5.2435.Peer-Reviewed Original ResearchConceptsMHC IInduction of transportersEndothelial cellsNK-resistant cell linesNatural killer-mediated lysisMajor histocompatibility complex INK inhibitory receptorsNK effector cellsPretreatment of ECB-lymphoblastoid cell linesEC sensitivityDermal microvascular endothelial cellsMicrovascular endothelial cellsCell linesHuman umbilical vein endothelial cellsExpression/functionUmbilical vein endothelial cellsAdenoviral-mediated transductionVascular vulnerabilityVein endothelial cellsNK assaysEffector cellsEndothelial activationInhibitory receptorsDeficient targets
1997
Negative Regulation by HLA-DO of MHC Class II-Restricted Antigen Processing
Denzin L, Sant'Angelo D, Hammond C, Surman M, Cresswell P. Negative Regulation by HLA-DO of MHC Class II-Restricted Antigen Processing. Science 1997, 278: 106-109. PMID: 9311912, DOI: 10.1126/science.278.5335.106.Peer-Reviewed Original ResearchConceptsHLA-DOII-like moleculeAntigen processingClass IIHLA-DMMajor histocompatibility complex classMHC class IIHistocompatibility complex classClass II moleculesII-CLIP complexesHLA-DM functionT cell linesB cellsChain-derived peptideAntigenic peptidesImportant modulatorCell linesDM functionInvariant chain-derived peptidesComplex classNegative regulationPeptidesMisfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome
Hughes E, Hammond C, Cresswell P. Misfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1896-1901. PMID: 9050876, PMCID: PMC20014, DOI: 10.1073/pnas.94.5.1896.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcysteineBeta 2-MicroglobulinBlotting, WesternCysteine EndopeptidasesCysteine Proteinase InhibitorsCytoplasmEndoplasmic ReticulumEnzyme InhibitorsGlycosylationHistocompatibility Antigens Class IHumansKineticsLeupeptinsMultienzyme ComplexesProteasome Endopeptidase ComplexProtein FoldingSolubilityTransfectionTumor Cells, CulturedConceptsClass I heavy chainsMHC class I heavy chainMajor histocompatibility complex class I heavy chainsBeta2-microglobulinHeavy chainMHC class ICell linesCell line DaudiTAP-deficient cell linesSpecific irreversible inhibitorClass IHerpes simplex virus proteinDaudiVirus proteinsEndoplasmic reticulumIrreversible inhibitorSimilar accumulationLactacystinPeriod of hours
1996
HLA-DM Interactions with Intermediates in HLA-DR Maturation and a Role for HLA-DM in Stabilizing Empty HLA-DR Molecules
Denzin L, Hammond C, Cresswell P. HLA-DM Interactions with Intermediates in HLA-DR Maturation and a Role for HLA-DM in Stabilizing Empty HLA-DR Molecules. Journal Of Experimental Medicine 1996, 184: 2153-2166. PMID: 8976171, PMCID: PMC2196380, DOI: 10.1084/jem.184.6.2153.Peer-Reviewed Original ResearchConceptsClass II moleculesHLA-DR moleculesHLA-DMDR moleculesMajor histocompatibility complex classAntigenic peptidesEmpty class II moleculesCLIP dissociationHistocompatibility complex classMHC class II compartmentsHLA-DM moleculesClass II compartmentsII-CLIP complexesClass II dimersAlpha beta dimersAntigen processingBeta dimersDR complexesCell linesInvariant chain fragmentComplex classAssociationLow levels
1995
Different MHC class I alleles compete for presentation of overlapping viral epitopes
Tussey L, Rowland-Jones S, Zheng T, Androlewicz M, Cresswell P, Frelinger J, McMichael A. Different MHC class I alleles compete for presentation of overlapping viral epitopes. Immunity 1995, 3: 65-77. PMID: 7542549, DOI: 10.1016/1074-7613(95)90159-0.Peer-Reviewed Original ResearchHLA-DM induces clip dissociation from MHC class II αβ dimers and facilitates peptide loading
Denzin L, Cresswell P. HLA-DM induces clip dissociation from MHC class II αβ dimers and facilitates peptide loading. Cell 1995, 82: 155-165. PMID: 7606781, DOI: 10.1016/0092-8674(95)90061-6.Peer-Reviewed Original Research
1992
HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides
Riberdy J, Newcomb J, Surman M, Barbosat J, Cresswell P. HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides. Nature 1992, 360: 474-477. PMID: 1448172, DOI: 10.1038/360474a0.Peer-Reviewed Original ResearchConceptsClass II moleculesInvariant chainMajor histocompatibility complex (MHC) class II moleculesCell linesHLA-DR moleculesClass II binding siteInvariant chain peptideHLA-DR3 moleculesMore MHCClass IIAntigen processingT2 transfectantsAntigenic peptidesHuman cell linesChain peptideVivo conditionsPeptidesLarge proportionProteolytic cleavageMutant cell linesCertain human cell linesEarly stagesTransfectants
1989
Differential transport requirements of HLA and H-2 class I glycoproteins
Alexander J, Payne J, Murray R, Frelinger J, Cresswell P. Differential transport requirements of HLA and H-2 class I glycoproteins. Immunogenetics 1989, 29: 380-388. PMID: 2731965, DOI: 10.1007/bf00375866.Peer-Reviewed Original Research
1987
Regulation of HLA Class I and Class II antigen expression
Cresswell P. Regulation of HLA Class I and Class II antigen expression. British Medical Bulletin 1987, 43: 66-80. PMID: 3315102, DOI: 10.1093/oxfordjournals.bmb.a072177.Peer-Reviewed Original ResearchConceptsCell surfaceRegulatory genesTranscriptional levelHybridisation experimentsIntracellular transportThird glycoproteinHeterodimeric glycoproteinCell typesChondroitin sulfate proteoglycanEfficient assemblyCell linesSulfate proteoglycanEnhanced expressionT cell linesGenesClass IExpressionTissue distributionRegulationLymphoblastoid linesGlycoproteinUnusual complexityClass I moleculesInvariant chainBiosynthesis
1986
Expression of differentiation antigens by hybrids of human lymphoblastoid cells
Howell D, Salter R, Koppelman B, Cresswell P. Expression of differentiation antigens by hybrids of human lymphoblastoid cells. Human Immunology 1986, 17: 443-455. PMID: 3098709, DOI: 10.1016/0198-8859(86)90303-4.Peer-Reviewed Original ResearchConceptsParent cell lineCell linesClass IClass II antigensB lymphocyte antigensClass II histocompatibility antigensLymphocyte differentiation markersT-lymphoblastoid cell lineII antigensHistocompatibility antigensLymphocyte antigenDifferentiation antigensLymphoblastoid cell linesParental antigensHNK-1AntigenMonoclonal antibodiesHuman lymphoblastoid cellsT-LCLDifferentiation markersLymphoblastoid cellsCurrent study
1985
Evidence for methylation as a regulatory mechanism in HLA-DRx gene expression
Carrington M, Salter R, Cresswell P, Ting J. Evidence for methylation as a regulatory mechanism in HLA-DRx gene expression. Immunogenetics 1985, 22: 219-229. PMID: 2995246, DOI: 10.1007/bf00404481.Peer-Reviewed Original ResearchConceptsGene expressionDNA methylationLymphoblastoid cell linesRegulatory mechanismsDNA hypomethylationParental linesHybrid linesSomatic cell hybridsRestriction enzyme Hpa IIHpa II digestionMsp I siteDifferent regulatory mechanismsCell hybridsGenomic probesGene inductionUnexpressed genesComplete demethylationHpa IIGenesDR genesMethylationT-LCLCell linesI siteHypomethylationGenes regulating HLA class I antigen expression in T-B lymphoblast hybrids
Salter R, Howell D, Cresswell P. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 1985, 21: 235-246. PMID: 3872841, DOI: 10.1007/bf00375376.Peer-Reviewed Original ResearchConceptsHLA class IClass II antigen expressionClass II antigensHLA-B antigensClass III antigensAntigen expressionLymphoblastoid cell linesTotal HLA class IHLA class II antigensHLA-A2 antigenHLA-B5 antigenHLA-A antigensB-lymphoblastoid cell linesCell linesHLA-DRHLA-B5HLA-A2B-LCLParental cell lineAntigenEnhanced expressionDecreased amountCEMRChromosome 6
1984
Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids
Howell D, Kostyu D, Ting J, Cresswell P. Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids. Somatic Cell And Molecular Genetics 1984, 10: 217-224. PMID: 6609440, DOI: 10.1007/bf01535244.Peer-Reviewed Original ResearchConceptsClass I histocompatibilityB-LCLLymphoblastoid cell linesB antigensPaucity of HLAHLA class I heavy chainsHLA-B antigensHLA-A antigensB-lymphoblastoid cell linesB antigen expressionClass I heavy chainsAntigen expressionB expressionHLAIndirect immunofluorescenceAntigenLow levelsClass IT-LCLHistocompatibilityCell linesHeavy chain mRNATotal levelsCEMRHeavy chainHLA-DR7-Specific monoclonal antibodies and a chimpanzee anti-DR7 serum detect different epitopes on the same molecule
Radka S, Bernard Amos D, Quackenbush L, Cresswell P. HLA-DR7-Specific monoclonal antibodies and a chimpanzee anti-DR7 serum detect different epitopes on the same molecule. Immunogenetics 1984, 19: 63-76. PMID: 6198273, DOI: 10.1007/bf00364476.Peer-Reviewed Original ResearchConceptsClass II moleculesChimpanzee antiserumSame class II moleculesDR7 specificitiesSerologic specificityMonoclonal antibodiesBinding-inhibition studiesCell linesSame serologic specificityAllogeneic epitopesNoncytotoxic antibodiesHLA-DR7IgM antibodiesSpecific monoclonal antibodiesDR moleculesDifferent epitopesAntibodiesEpitopesBeta chainCytotoxic activityHomozygous cell linesAntiserumMembrane preparationsCytotoxicitySpecificity
1981
A human leukocyte antigen identified by a monoclonal antibody
Berger A, Davis J, Cresswell P. A human leukocyte antigen identified by a monoclonal antibody. Human Immunology 1981, 3: 231-245. PMID: 6975769, DOI: 10.1016/0198-8859(81)90020-3.Peer-Reviewed Original ResearchConceptsLeukocyte antigenT cell linesB cell linesB lymphocytesCell linesMonoclonal antibodiesHuman leukocyte antigenPeripheral blood cellsBone marrow cellsAllogeneic cellsT cellsB cellsErythroid seriesMore antigensAntigenHuman leukocytesMarrow cellsBlood cellsNegative populationAntibodiesLymphocytesApproximate molecular weightCellsElectrophoretic mobilityMonocytes
1980
HLA-A2 as a target for cell-mediated lympholysis: Evidence from immunoselected HLA-A2 negative mutant cell lines
Andreotti P, Apgar J, Cresswell P. HLA-A2 as a target for cell-mediated lympholysis: Evidence from immunoselected HLA-A2 negative mutant cell lines. Human Immunology 1980, 1: 77-86. PMID: 7263311, DOI: 10.1016/0198-8859(80)90011-7.Peer-Reviewed Original ResearchConceptsCytotoxic T lymphocytesCell-mediated lympholysisHLA-A2T lymphocytesMixed lymphocyte responseHLA-A2 mutantsCell linesLymphocyte responsesHLA typingImmune precipitation analysisLymphocytesTarget cellsMolecular targetsSensitized cellsMutant cell linesComparable susceptibilityLympholysisHLAStaphylococcus aureus proteinPutative targetsAureus proteinNegative selectionCellsTargetSusceptibility
1979
Transferrin receptors on human B and T lymphosblastoid cell lines
Larrick J, Cresswell P. Transferrin receptors on human B and T lymphosblastoid cell lines. Biochimica Et Biophysica Acta 1979, 583: 483-490. PMID: 218639, DOI: 10.1016/0304-4165(79)90065-5.Peer-Reviewed Original ResearchModulation of cell surface iron transferrin receptors by cellular density and state of activation
Larrick J, Cresswell P. Modulation of cell surface iron transferrin receptors by cellular density and state of activation. Journal Of Cellular Biochemistry 1979, 11: 579-586. PMID: 232525, DOI: 10.1002/jss.400110415.Peer-Reviewed Original ResearchConceptsLymphoid cell linesTransferrin receptorNormal peripheral blood lymphocytesCell linesNormal resting lymphocytesMixed lymphocyte culturePeripheral blood lymphocytesNumber of receptorsState of activationBlood lymphocytesNumber of transferrinResting lymphocytesNormal lymphocytesLymphocyte culturesCell activationContinuous cell linesLymphocytesReceptorsNormal human fibroblastsNormal cellsCellular density
1977
Human B cell alloantigens: separation from other membrane molecules by affinity chromatography
Cresswell P. Human B cell alloantigens: separation from other membrane molecules by affinity chromatography. European Journal Of Immunology 1977, 7: 636-639. PMID: 303569, DOI: 10.1002/eji.1830070911.Peer-Reviewed Original ResearchConceptsMolecular weight 35 000Sodium dodecyl sulfate gel electrophoresisDodecyl sulfate gel electrophoresisAffinity chromatographySulfate gel electrophoresisSoluble productsB-lymphoblastoid cell linesChromatographyMoleculesSodium deoxycholateLymphoblastoid cell linesGel electrophoresisAgarose columnIa-like alloantigensDeoxycholateCell linesHuman B cellsSeparationSpecific rabbit antibodiesB cellsColumnSerological activityElectrophoresisRabbit antibodiesMaterials