Large-scale analysis of the yeast genome by transposon tagging and gene disruption
Ross-Macdonald P, Coelho P, Roemer T, Agarwal S, Kumar A, Jansen R, Cheung K, Sheehan A, Symoniatis D, Umansky L, Heidtman M, Nelson F, Iwasaki H, Hager K, Gerstein M, Miller P, Roeder G, Snyder M. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 1999, 402: 413-418. PMID: 10586881, DOI: 10.1038/46558.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsDNA Transposable ElementsEscherichia coliFungal ProteinsGene Expression ProfilingGenetic TechniquesGenome, FungalMolecular Sequence DataMutagenesisOligonucleotide Array Sequence AnalysisOpen Reading FramesPhenotypePolymerase Chain ReactionSaccharomyces cerevisiaeTransformation, GeneticConceptsGene functionDisruption phenotypesLarge-scale analysisNon-annotated open reading framesGenome-wide analysisOpen reading frameSingle genetic backgroundTransposon taggingYeast genomeYeast mutantsGenomic scaleYeast SaccharomycesDifferent growth conditionsProtein localizationGene disruptionReading frameGene expressionFunctional analysisVegetative growthGenetic backgroundGenesGenomeTransposonGrowth conditionsYeast