2019
Protein Engineering and Glycan Optimization Improves Pharmicokinetics of an Enzyme Biologic 10‐fold
Braddock D, Stabach P, Zimmerman K, Kavanagh D, Sauei C, Yarema K. Protein Engineering and Glycan Optimization Improves Pharmicokinetics of an Enzyme Biologic 10‐fold. The FASEB Journal 2019, 33: 801.1-801.1. DOI: 10.1096/fasebj.2019.33.1_supplement.801.1.Peer-Reviewed Original ResearchFusion proteinProtein engineeringCHO cell linesCellular recyclingBioprocessing methodsRecombinant fusion proteinConsensus sequenceExtracellular domainSialic acidTertiary structureN-acetylmannosamineCHO cellsHuman alphaProteinCell linesFASEB JournalFc domainCell culture mediumCulture mediumGeneralized Arterial CalcificationENPP1
2008
Ankyrin facilitates intracellular trafficking of α1-Na+-K+-ATPase in polarized cells
Stabach PR, Devarajan P, Stankewich MC, Bannykh S, Morrow JS. Ankyrin facilitates intracellular trafficking of α1-Na+-K+-ATPase in polarized cells. American Journal Of Physiology - Cell Physiology 2008, 295: c1202-c1214. PMID: 18768923, PMCID: PMC2584975, DOI: 10.1152/ajpcell.00273.2008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnkyrin RepeatAnkyrinsCell PolarityChlorocebus aethiopsCOS CellsCytoplasmDogsEndoplasmic ReticulumGolgi ApparatusHumansMembrane GlycoproteinsMonomeric GTP-Binding ProteinsProtein ConformationProtein FoldingProtein Sorting SignalsProtein TransportRatsRecombinant Fusion ProteinsRNA InterferenceRNA, Small InterferingSodium-Potassium-Exchanging ATPaseTime FactorsTransfectionViral Envelope ProteinsConceptsEndoplasmic reticulumSecretory pathwayPlasma membraneVesicular stomatitis virus G proteinMadin-Darby canine kidney cellsVirus G proteinSmall hairpin RNACanine kidney cellsGolgi traffickingAnkyrin RGolgi transportSemipermeabilized cellsER retentionCytoplasmic domainMembrane proteinsAssembly pathwayProtein ankyrinIntracellular traffickingAnkyrinFusion proteinSimilar phenotypeG proteinsSuch phenotypesHairpin RNACultured cells