2021
Regulation of RNA polymerase II activity is essential for terminal erythroid maturation
Murphy ZC, Murphy K, Myers J, Getman M, Couch T, Schulz VP, Lezon-Geyda K, Palumbo C, Yan H, Mohandas N, Gallagher PG, Steiner LA. Regulation of RNA polymerase II activity is essential for terminal erythroid maturation. Blood 2021, 138: 1740-1756. PMID: 34075391, PMCID: PMC8569412, DOI: 10.1182/blood.2020009903.Peer-Reviewed Original ResearchConceptsRNA polymerase IIRNA polymerase II activityTerminal erythroid maturationPolymerase II activityPolymerase IIErythroid maturationHuman erythroblastsGene expressionTerminal maturationII activityStage-specific regulationHistone posttranslational modificationsTransposase-accessible chromatinErythroid-specific genesAccumulation of heterochromatinHigh-throughput sequencingLevel of transcriptionLate-stage erythroblastsEssential biologic processesAccessible chromatinHistone marksTranscription elongationChromatin structureTranscriptional repressionChromatin immunoprecipitation
2019
Terminal Erythroid Maturation Is Associated with Dynamic Changes in the Abundance of Histone Marks Associated with Active Transcription Elongation and RNA Polymerase II Pausing
Murphy Z, Couch T, Lillis J, Getman M, Lezon-Geyda K, Schulz V, Narla M, Gallagher P, Steiner L. Terminal Erythroid Maturation Is Associated with Dynamic Changes in the Abundance of Histone Marks Associated with Active Transcription Elongation and RNA Polymerase II Pausing. Blood 2019, 134: 154. DOI: 10.1182/blood-2019-129561.Peer-Reviewed Original ResearchRNA polymerase II pausingTerminal erythroid maturationPol IIC-terminal domainErythroid maturationGene expressionTranscription elongationPosttranslational modificationsSpecific histone posttranslational modificationsPol II C-terminal domainRNA polymerase II pause releaseKey regulatorHistone H4 lysine 16 acetylationHistone H4 lysine 20Histone post-translational modificationsH4 lysine 16 acetylationHistone H3 lysine 79H4 lysine 20Histone posttranslational modificationsRNA Pol IIH3 lysine 79Multiple histone marksPost-translational modificationsTranscription start siteChIP-seq data
2008
Genome-Wide Analysis of EKLF Occupancy in Erythroid Chromatin Reveals 5′, 3′ and Intragenic Binding Sites in EKLF Target Genes
Pilon A, Margulies E, Abaan H, Allen A, Townes T, Frederick A, Zhou D, Gallagher P, Bodine D. Genome-Wide Analysis of EKLF Occupancy in Erythroid Chromatin Reveals 5′, 3′ and Intragenic Binding Sites in EKLF Target Genes. Blood 2008, 112: 283. DOI: 10.1182/blood.v112.11.283.283.Peer-Reviewed Original ResearchCell cycle networkChIP-seqIngenuity Pathway AnalysisTranscription factorsEKLF geneWide analysisIntragenic sitesTarget genesChIP-PCRErythroid cellsMapping protein-DNA interactionsErythroid Kruppel-like factorFetal liver erythroid cellsGenome-wide scaleBeta-globin locusGenome-wide analysisCell cycle control networkProtein-DNA interactionsChIP-seq resultsTerminal erythroid maturationDNase hypersensitive sitesKnowledge of genesChIP-seq librariesChIP-seq dataFL cells
2005
Genomic Organization, Chromatin Structure, and Transcriptional Regulation of the Murine Alpha Hemoglobin Stabilizing Protein (AHSP) Gene.
Dore L, Vakoc C, Blobel G, Hardison R, Bodine D, Gallagher P, Weiss M. Genomic Organization, Chromatin Structure, and Transcriptional Regulation of the Murine Alpha Hemoglobin Stabilizing Protein (AHSP) Gene. Blood 2005, 106: 3633. DOI: 10.1182/blood.v106.11.3633.3633.Peer-Reviewed Original ResearchGATA-1AHSP geneChromatin structureHistone H3 lysine 4Alpha-Hemoglobin Stabilizing ProteinDNase I hypersensitive sitesGene regulatory domainsH3 lysine 4Kb genomic regionTerminal erythroid maturationGATA-1 bindingErythroid-specific expressionTransient transfection assaysInitial functional studiesAHSP promoterSyntenic blocksCandidate modifier genesGenomic organizationLysine 4Erythroid proteinGene regulationStabilizing ProteinTranscriptional regulationAdjacent genesGenomic regions