2021
Regulation of RNA polymerase II activity is essential for terminal erythroid maturation
Murphy ZC, Murphy K, Myers J, Getman M, Couch T, Schulz VP, Lezon-Geyda K, Palumbo C, Yan H, Mohandas N, Gallagher PG, Steiner LA. Regulation of RNA polymerase II activity is essential for terminal erythroid maturation. Blood 2021, 138: 1740-1756. PMID: 34075391, PMCID: PMC8569412, DOI: 10.1182/blood.2020009903.Peer-Reviewed Original ResearchConceptsRNA polymerase IIRNA polymerase II activityTerminal erythroid maturationPolymerase II activityPolymerase IIErythroid maturationHuman erythroblastsGene expressionTerminal maturationII activityStage-specific regulationHistone posttranslational modificationsTransposase-accessible chromatinErythroid-specific genesAccumulation of heterochromatinHigh-throughput sequencingLevel of transcriptionLate-stage erythroblastsEssential biologic processesAccessible chromatinHistone marksTranscription elongationChromatin structureTranscriptional repressionChromatin immunoprecipitation
2019
Terminal Erythroid Maturation Is Associated with Dynamic Changes in the Abundance of Histone Marks Associated with Active Transcription Elongation and RNA Polymerase II Pausing
Murphy Z, Couch T, Lillis J, Getman M, Lezon-Geyda K, Schulz V, Narla M, Gallagher P, Steiner L. Terminal Erythroid Maturation Is Associated with Dynamic Changes in the Abundance of Histone Marks Associated with Active Transcription Elongation and RNA Polymerase II Pausing. Blood 2019, 134: 154. DOI: 10.1182/blood-2019-129561.Peer-Reviewed Original ResearchRNA polymerase II pausingTerminal erythroid maturationPol IIC-terminal domainErythroid maturationGene expressionTranscription elongationPosttranslational modificationsSpecific histone posttranslational modificationsPol II C-terminal domainRNA polymerase II pause releaseKey regulatorHistone H4 lysine 16 acetylationHistone H4 lysine 20Histone post-translational modificationsH4 lysine 16 acetylationHistone H3 lysine 79H4 lysine 20Histone posttranslational modificationsRNA Pol IIH3 lysine 79Multiple histone marksPost-translational modificationsTranscription start siteChIP-seq data