2009
Genome-Wide ChIP-Seq Reveals a Dramatic Shift in the EKLF Binding Profile Between Erythroid Progenitors and Erythroblasts.
Pilon A, Ajay S, Abaan H, Margulies E, Gallagher P, Bodine D. Genome-Wide ChIP-Seq Reveals a Dramatic Shift in the EKLF Binding Profile Between Erythroid Progenitors and Erythroblasts. Blood 2009, 114: 565. DOI: 10.1182/blood.v114.22.565.565.Peer-Reviewed Original ResearchTarget genesChIP-seqNearest geneSimultaneous genome-wide analysisC2H2 zinc finger transcription factorGenome-wide ChIP-seqErythroid Kruppel-like factorZinc finger transcription factorErythroid progenitorsTranscription factor occupancyBeta-globin locusGenome-wide analysisCell cycle control factorsProtein-DNA interactionsFinger transcription factorChIP-seq dataS-phase entryNon-repetitive regionsKruppel-like factorEKLF geneEKLF proteinFetal liverCell cycle S-phase entryChromatin remodelingFactor occupancy
2008
Genome-Wide Analysis of EKLF Occupancy in Erythroid Chromatin Reveals 5′, 3′ and Intragenic Binding Sites in EKLF Target Genes
Pilon A, Margulies E, Abaan H, Allen A, Townes T, Frederick A, Zhou D, Gallagher P, Bodine D. Genome-Wide Analysis of EKLF Occupancy in Erythroid Chromatin Reveals 5′, 3′ and Intragenic Binding Sites in EKLF Target Genes. Blood 2008, 112: 283. DOI: 10.1182/blood.v112.11.283.283.Peer-Reviewed Original ResearchCell cycle networkChIP-seqIngenuity Pathway AnalysisTranscription factorsEKLF geneWide analysisIntragenic sitesTarget genesChIP-PCRErythroid cellsMapping protein-DNA interactionsErythroid Kruppel-like factorFetal liver erythroid cellsGenome-wide scaleBeta-globin locusGenome-wide analysisCell cycle control networkProtein-DNA interactionsChIP-seq resultsTerminal erythroid maturationDNase hypersensitive sitesKnowledge of genesChIP-seq librariesChIP-seq dataFL cells
2005
The Human Alpha Hemoglobin Stabilizing Protein (AHSP) Gene Locus in EKLF-Deficient Erythroid Cells.
Pilon A, Zhou D, Weiss M, Townes T, Bodine D, Gallagher P. The Human Alpha Hemoglobin Stabilizing Protein (AHSP) Gene Locus in EKLF-Deficient Erythroid Cells. Blood 2005, 106: 1740. DOI: 10.1182/blood.v106.11.1740.1740.Peer-Reviewed Original ResearchCACCC siteErythroid cellsWild typeModifier genesDNase I hypersensitive site mappingLocal chromatin structureΒ-globin transcriptionErythroid-specific proteinΒ-globin gene promoterDNA-protein complexesFetal liver cellsTranscription factor EKLFChromatin immunoprecipitation analysisΒ-globin sequencesHypersensitive site mappingFetal liverMobility shift assaysPromoter-luciferase reporter plasmidProximal CACCC boxFetal liver DNACore promoter regionAHSP promoterDeficient fetal liver cellsEKLF geneChromatin modulators