2002
Role of c-SRC and ERK in acid-induced activation of NHE3
Tsuganezawa H, Sato S, Yamaji Y, Preisig PA, Moe OW, Alpern RJ. Role of c-SRC and ERK in acid-induced activation of NHE3. Kidney International 2002, 62: 41-50. PMID: 12081562, DOI: 10.1046/j.1523-1755.2002.00418.x.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmonium ChlorideAnimalsCells, CulturedCSK Tyrosine-Protein KinaseEnzyme ActivationJNK Mitogen-Activated Protein KinasesMaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSrc-Family KinasesConceptsOKP cellsAcid-induced activationNHE3 activityERK/c-fosC-fos expressionC-Fos activationCytoplasmic pH (pHi) recoveryC-SrcRenal proximal tubulesAcid incubationActivation of NHE3Renal epithelial cellsCultured renal epithelial cellsProximal tubulesChronic acidosisInhibition of MEKNHE3 activationInhibited activationNormal levelsAcid loadImmune complex kinase assayERK kinaseAcidosisPH recoveryEpithelial cells
2000
Acid incubation causes exocytic insertion of NHE3 in OKP cells
Yang X, Amemiya M, Peng Y, Moe O, Preisig P, Alpern R. Acid incubation causes exocytic insertion of NHE3 in OKP cells. American Journal Of Physiology - Cell Physiology 2000, 279: c410-c419. PMID: 10913008, DOI: 10.1152/ajpcell.2000.279.2.c410.Peer-Reviewed Original Research
1999
Glucocorticoids enhance acid activation of the Na+/H+ exchanger 3 (NHE3)
Ambühl P, Yang X, Peng Y, Preisig P, Moe O, Alpern R. Glucocorticoids enhance acid activation of the Na+/H+ exchanger 3 (NHE3). Journal Of Clinical Investigation 1999, 103: 429-435. PMID: 9927505, PMCID: PMC407891, DOI: 10.1172/jci2913.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceM hydrocortisoneNHE3 activityAcid incubationNHE3 mRNA abundanceTrafficking of NHE3Absence of hydrocortisoneOpossum kidney cellsExogenous glucocorticoidsExchanger 3 (NHE3) activityHydrocortisoneProtein abundanceGlucocorticoidsProtein synthesisExchanger 3Kidney cellsTwofold increaseMRNA abundanceApical membrane