2024
Lateral expansion of the mammalian cerebral cortex is related to anchorage of centrosomes in apical neural progenitors
Morozov Y, Rakic P. Lateral expansion of the mammalian cerebral cortex is related to anchorage of centrosomes in apical neural progenitors. Cerebral Cortex 2024, 34: bhae293. PMID: 39024157, PMCID: PMC11485267, DOI: 10.1093/cercor/bhae293.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsProgenitor cellsVentricular zoneCerebral cortexBasolateral cell membraneApical anchorageProlonged neurogenesisMammalian cerebral cortexPrimary ciliaApical neural progenitorsCell membraneFraction of cellsNeural progenitorsStem cellsCerebral neurogenesisApical segmentsDevelopment of ciliaNuclear translocationMicrotubule organizing centerNeurogenesisCellsMacaque monkeysSpecies-specific differencesCortexBasal bodies
2022
Neural Stem Cells in Adult Mammals are not Astrocytes
Velloso F, Shankar S, Parpura V, Rakic P, Levison S. Neural Stem Cells in Adult Mammals are not Astrocytes. ASN Neuro 2022, 14: 17590914221134739. PMID: 36330653, PMCID: PMC9638700, DOI: 10.1177/17590914221134739.Peer-Reviewed Original ResearchConceptsAdult mammalian subventricular zoneNeural stem cellsMammalian subventricular zoneMammalian neural stem cellsComparative transcriptomic analysisDistinct gene expression profilesStem cellsAdult mammalian neural stem cellsGene expression profilesSingle-cell RNAseqFunction of NSCsMurine neural stem cellsSubventricular zoneTranscriptomic analysisExpression profilesAdult murine neural stem cellsCell sortingAdult mammalsFunction of astrocytesCellsNew neuronsSubtypes of astrocytesMammalsRNAseqNiche
2014
Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia
Brennand K, Savas J, Kim Y, Tran N, Simone A, Hashimoto-Torii K, Beaumont K, Kim H, Topol A, Ladran I, Abdelrahim M, Matikainen-Ankney B, Chao S, Mrksich M, Rakic P, Fang G, Zhang B, Yates J, Gage F. Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia. Molecular Psychiatry 2014, 20: 361-368. PMID: 24686136, PMCID: PMC4182344, DOI: 10.1038/mp.2014.22.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntipsychotic AgentsCell DifferentiationCell MovementCells, CulturedFemaleGene ExpressionHumansMaleMiceMice, Inbred C57BLMice, TransgenicMitochondriaNeural Cell Adhesion MoleculesNeural Stem CellsOxidative StressPhenotypePluripotent Stem CellsProsencephalonProteomicsReactive Oxygen SpeciesSchizophreniaYoung AdultConceptsHiPSC neural progenitor cellsNeural progenitor cellsHuman-induced pluripotent stem cellsHiPSC-derived neuronsGene expressionGene expression comparisonsStable isotope labelingProteomic mass spectrometry analysisAbnormal gene expressionPluripotent stem cellsOxidative stressCytoskeletal remodelingMass spectrometry analysisCellular phenotypesExpression comparisonsDevelopmental mechanismsIsotope labelingPhenotypic differencesBrainSpan AtlasDisease predispositionAmino acidsScalable assayNPC phenotypeStem cellsProgenitor cells
2000
Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain
Laywell E, Rakic P, Kukekov V, Holland E, Steindler D. Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 13883-13888. PMID: 11095732, PMCID: PMC17670, DOI: 10.1073/pnas.250471697.Peer-Reviewed Original ResearchConceptsCentral nervous systemNeural stem cellsAstrocyte monolayersMultipotent astrocytic stem cellsAstrocytic stem cellsPostnatal central nervous systemAdult mouse brainStem cellsCerebral cortexPostnatal wkSpinal cordAdult brainSubependymal zoneNervous systemEpendymal cellsMouse brainMammalian brainSpherical clonesCNS developmentBrainVivo identificationAstrocytesMature forebrainCritical periodCE cells
1999
Continuation of neurogenesis in the hippocampus of the adult macaque monkey
Kornack D, Rakic P. Continuation of neurogenesis in the hippocampus of the adult macaque monkey. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 5768-5773. PMID: 10318959, PMCID: PMC21935, DOI: 10.1073/pnas.96.10.5768.Peer-Reviewed Original ResearchConceptsAdult macaque monkeysDentate gyrusMacaque monkeysAdult primate hippocampusAdult rodent dentate gyrusImmunohistochemical double labelingHippocampal dentate gyrusRodent dentate gyrusDiscreet brain regionsMultipotent neural stem cellsNeural stem cellsAdult neurogenesisAdult brainPrimate hippocampusAnimal modelsBrain regionsDouble labelingNeurogenesisSpecific markersHippocampusGyrusStem cellsCell typesMonkeysHumans
1997
Restrictive clonal allocation in the chimeric mouse brain
Kuan C, Elliott E, Flavell R, Rakic P. Restrictive clonal allocation in the chimeric mouse brain. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 3374-3379. PMID: 9096401, PMCID: PMC20377, DOI: 10.1073/pnas.94.7.3374.Peer-Reviewed Original ResearchConceptsBrain regionsChimeric mouse brainsMammalian cerebral cortexDifferent brain regionsCerebral cortexCerebral neocortexChimeric miceMouse brainChimeric brainsMammalian brainProgenitor cellsBrainClones of cellsNeocortexStem cellsBlastocyst embryosCellsDifferent subsetsVast majorityCell descendantsPrevious studiesCortexMice
1988
Nature and fate of proliferative cells in the hippocampal dentate gyrus during the life span of the rhesus monkey
Eckenhoff M, Rakic P. Nature and fate of proliferative cells in the hippocampal dentate gyrus during the life span of the rhesus monkey. Journal Of Neuroscience 1988, 8: 2729-2747. PMID: 3411351, PMCID: PMC6569394, DOI: 10.1523/jneurosci.08-08-02729.1988.Peer-Reviewed Original ResearchConceptsProliferative cellsMitotic divisionPostmitotic cellsUltra-structural characteristicsPossible functionsElectron microscopic levelStable populationGlial fibrillary acidic proteinNeuronal cellsStem cellsJuvenile periodMajor classesMature primatesSubgranular zoneDentate gyrusGlial cellsLife spanCellsGFAP-negative cellsFateRhesus monkeysAcidic proteinNew neuronsPostpubertal animalsFibrillary acidic protein