2020
RNA-binding proteins in neurological development and disease
Prashad S, Gopal PP. RNA-binding proteins in neurological development and disease. RNA Biology 2020, 18: 972-987. PMID: 32865115, PMCID: PMC8216196, DOI: 10.1080/15476286.2020.1809186.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsRNA-binding proteinGene expressionDisease-linked RNA-binding proteinsHigh-throughput transcriptomic analysisRNA processing stepsAberrant RNA metabolismAberrant RNA processingRecent exciting studiesRNA processingRNA metabolismMRNA traffickingAlternative splicingMultifunctional proteinTranscriptomic analysisSplicing misregulationMRNA stabilityMolecular mechanismsProtein resultsNeuronal homeostasisNeurodevelopmental defectsNeuronal proteinsFunctional consequencesPleiotropic effectsSpatiotemporal controlProtein
2019
Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons
Stavoe A, Gopal P, Gubas A, Tooze S, Holzbaur E. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. ELife 2019, 8: e44219. PMID: 31309927, PMCID: PMC6634969, DOI: 10.7554/elife.44219.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisAged miceNovel therapeutic targetLate-onset neurodegenerative diseasesAge-associated neurodegenerationAge-related declineAged neuronsRisk factorsLateral sclerosisParkinson's diseaseTherapeutic targetLC3B-positive autophagosomesNeurodegenerative diseasesMammalian neuronsNeuronsSignificant decreaseDiseaseRate of autophagy
2017
Amyotrophic lateral sclerosis-linked mutations increase the viscosity of liquid-like TDP-43 RNP granules in neurons
Gopal PP, Nirschl JJ, Klinman E, Holzbaur EL. Amyotrophic lateral sclerosis-linked mutations increase the viscosity of liquid-like TDP-43 RNP granules in neurons. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e2466-e2475. PMID: 28265061, PMCID: PMC5373408, DOI: 10.1073/pnas.1614462114.Peer-Reviewed Original ResearchConceptsRNA-binding proteinRNP granulesAmyotrophic lateral sclerosis-linked mutationsCritical cellular processesRapid fluorescence recoveryAmyotrophic lateral sclerosisBiophysical propertiesDistinct biophysical propertiesRibonucleoprotein granulesSubcellular contextCellular processesSpecific RNAToxic gainTAR-DNA binding protein 43Superresolution microscopyFluorescence recoveryLiquid-liquid phase separationMutant TDP-43Primary cortical neuronsLiquid-like propertiesTDP-43RNAProteinMutationsAxonal location
2014
MicroRNA-124 expression counteracts pro-survival stress responses in glioblastoma
Mucaj V, Lee S, Skuli N, Giannoukos D, Qiu B, Eisinger-Mathason T, Nakazawa M, Shay J, Gopal P, Venneti S, Lal P, Minn A, Simon M, Mathew L. MicroRNA-124 expression counteracts pro-survival stress responses in glioblastoma. Oncogene 2014, 34: 2204-2214. PMID: 24954504, PMCID: PMC4275412, DOI: 10.1038/onc.2014.168.Peer-Reviewed Original ResearchConceptsMiR-124 expressionMiR-124 levelsGlioblastoma patient samplesTumor cell survivalPatient samplesAggressive adult brain tumorCell survivalCell deathSurvival of miceAdult brain tumorsHypoxic gene signaturePoor patient outcomesIntracranial xenograft tumorsHypoxic cell deathIncreases cell deathPatient outcomesTumor ischemiaXenograft tumorsBrain tumorsGlioblastoma patientsIschemic regionTherapeutic resistanceTumor progressionCancer typesGene signature