2011
Gbx2 and Fgf8 are sequentially required for formation of the midbrain-hindbrain compartment boundary
Sunmonu N, Li K, Guo Q, Li J. Gbx2 and Fgf8 are sequentially required for formation of the midbrain-hindbrain compartment boundary. Development 2011, 138: 725-734. PMID: 21266408, PMCID: PMC3026416, DOI: 10.1242/dev.055665.Peer-Reviewed Original ResearchConceptsMidbrain-hindbrain borderCompartment boundariesCell movementLineage boundariesCell-cell signalingPrevents cell movementsCell sortingGenetic inducible fate mappingExpression borderLineage-restriction boundariesCell surface characteristicsCell-autonomous effectsExpression of Otx2Inducible fate mappingFGF pathwayHomeobox genesGbx2Neural plateHindbrain fateFgf8Transverse bandsLineagesMidbrain progenitorsFate mappingHindbrain progenitors
2009
Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum
Guo Q, Li K, Sunmonu N, Li J. Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Developmental Biology 2009, 338: 183-192. PMID: 19968985, PMCID: PMC2815264, DOI: 10.1016/j.ydbio.2009.11.034.Peer-Reviewed Original ResearchConceptsGain-of-function studiesMHB regionMutant miceLoss-of-function analysesSplice-site mutationDouble mutant miceLoss of Fgf8In vivo requirementFunction of FGF8Alternative splicingProtein isoformsRegulatory genesActivation of Fgf8Fgf8 geneN-terminiSpliceformsGrowth retardationPostnatal lethalityGenetic backgroundMiceMidbrainFGF17Fgf8aCerebellumPosterior midbrainMisexpression of Gbx2 throughout the mesencephalon by a conditional gain‐of‐function transgene leads to deletion of the midbrain and cerebellum in mice
Sunmonu N, Chen L, Li J. Misexpression of Gbx2 throughout the mesencephalon by a conditional gain‐of‐function transgene leads to deletion of the midbrain and cerebellum in mice. Genesis 2009, 47: 667-673. PMID: 19603509, PMCID: PMC2783588, DOI: 10.1002/dvg.20546.Peer-Reviewed Original ResearchConceptsTransgenic mouse linesMouse homeobox genesMidbrain-hindbrain junctionCre-mediated recombinationPersistent expressionAbsence of CreEmbryonic dayMouse linesMouse embryosAdult brainExpression of Gbx2MiceAnterior shiftHomeobox genesNeural tubeMidbrainFunctional studiesPosterior partCerebellumRhombomere 1Ectopic expressionMesencephalonOtx2Red fluorescent proteinBeta-galactosidase