2024
De Novo Elastin Assembly Alleviates Development of Supravalvular Aortic Stenosis—Brief Report
Ellis M, Riaz M, Huang Y, Anderson C, Hoareau M, Li X, Luo H, Lee S, Park J, Luo J, Batty L, Huang Q, Lopez C, Reinhardt D, Tellides G, Qyang Y. De Novo Elastin Assembly Alleviates Development of Supravalvular Aortic Stenosis—Brief Report. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: 1674-1682. PMID: 38752350, PMCID: PMC11209776, DOI: 10.1161/atvbaha.124.320790.Peer-Reviewed Original ResearchSupravalvular aortic stenosisVascular smooth muscle cellsSmooth muscle cellsMuscle cellsAortic stenosisMedial vascular smooth muscle cellsVascular proliferative diseasesEpigallocatechin gallate treatmentProliferative abnormalitiesPreclinical findingsHeart failureLuminal occlusionMouse modelCell hyperproliferationDefective elastinProliferative diseasesCardiovascular disordersFormation of elastinTherapeutic interventionsElastin assemblyElastin depositionStenosisMiceAortic mechanicsImproper formation
2022
Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy
Khalilimeybodi A, Riaz M, Campbell S, Omens J, McCulloch A, Qyang Y, Saucerman J. Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy. Journal Of Molecular And Cellular Cardiology 2022, 174: 1-14. PMID: 36370475, PMCID: PMC10230857, DOI: 10.1016/j.yjmcc.2022.10.006.Peer-Reviewed Original ResearchMuscle LIM Protein Force-Sensing Mediates Sarcomeric Biomechanical Signaling in Human Familial Hypertrophic Cardiomyopathy
Riaz M, Park J, Sewanan LR, Ren Y, Schwan J, Das SK, Pomianowski PT, Huang Y, Ellis MW, Luo J, Liu J, Song L, Chen IP, Qiu C, Yazawa M, Tellides G, Hwa J, Young LH, Yang L, Marboe CC, Jacoby DL, Campbell SG, Qyang Y. Muscle LIM Protein Force-Sensing Mediates Sarcomeric Biomechanical Signaling in Human Familial Hypertrophic Cardiomyopathy. Circulation 2022, 145: 1238-1253. PMID: 35384713, PMCID: PMC9109819, DOI: 10.1161/circulationaha.121.056265.Peer-Reviewed Original ResearchConceptsHypertrophic cardiomyopathySarcomeric mutationsFamilial hypertrophic cardiomyopathySudden cardiac deathCardiac myosin heavy chainMechanism-based treatmentsDevelopment of hypertrophyActivated T cellsCalcineurin-nuclear factorForce productionPhenotypic expressionPluripotent stem cell-derived cardiomyocytesStem cell-derived cardiomyocytesHeart failureCardiac deathVentricular hypertrophyCell-derived cardiomyocytesCardiac contractilityPharmacological interventionsT cellsCardiac diseaseCardiac hypertrophyPatient-specific induced pluripotent stem cellsPharmacological meansTwitch relaxationReadily Available Tissue-Engineered Vascular Grafts Derived From Human Induced Pluripotent Stem Cells
Luo J, Qin L, Park J, Kural MH, Huang Y, Shi X, Riaz M, Wang J, Ellis MW, Anderson CW, Yuan Y, Ren Y, Yoder MC, Tellides G, Niklason LE, Qyang Y. Readily Available Tissue-Engineered Vascular Grafts Derived From Human Induced Pluripotent Stem Cells. Circulation Research 2022, 130: 925-927. PMID: 35189711, PMCID: PMC9113663, DOI: 10.1161/circresaha.121.320315.Peer-Reviewed Original Research
2021
Epigallocatechin gallate facilitates extracellular elastin fiber formation in induced pluripotent stem cell derived vascular smooth muscle cells for tissue engineering
Ellis MW, Riaz M, Huang Y, Anderson CW, Luo J, Park J, Lopez CA, Batty LD, Gibson KH, Qyang Y. Epigallocatechin gallate facilitates extracellular elastin fiber formation in induced pluripotent stem cell derived vascular smooth muscle cells for tissue engineering. Journal Of Molecular And Cellular Cardiology 2021, 163: 167-174. PMID: 34979103, PMCID: PMC8920537, DOI: 10.1016/j.yjmcc.2021.12.014.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsTissue engineeringStem cell derivativesPluripotent stem cell derivativesInduced pluripotent stem cellsStem cellsGraft productionMechanical strengthExtracellular formationExpression systemCell derivativesVascular smooth muscle cellsElastin fiber formationEngineered graftSmooth muscle cellsFiber formationNotable obstacleLack of elastinMuscle cellsEngineeringClinical applicationVascular graftsCell proliferative capacityElastin productionProliferative capacityMethods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Li ML, Luo J, Ellis MW, Riaz M, Ajaj Y, Qyang Y. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells. Methods In Molecular Biology 2021, 2375: 21-34. PMID: 34591296, DOI: 10.1007/978-1-0716-1708-3_3.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsVascular smooth muscle cellsPluripotent stem cellsLateral plate mesodermEarly embryonic developmentStem cellsSmooth muscle cellsHuman pluripotent stem cellsInduced pluripotent stem cellsExtracellular matrix proteinsMuscle cellsMesoderm lineagePlate mesodermEmbryonic developmentVascular cell sourceEmbryoid bodiesEB formationMatrix proteinsCellular interactionsDisease modelingPhysiological characteristicsVascular tissueTissue-engineered vascular graftsCell-based therapiesCell replacement
2020
Efficient Differentiation of Human Induced Pluripotent Stem Cells into Endothelial Cells under Xenogeneic-free Conditions for Vascular Tissue Engineering
Luo J, Shi X, Lin Y, Yuan Y, Kural MH, Wang J, Ellis MW, Anderson CW, Zhang SM, Riaz M, Niklason LE, Qyang Y. Efficient Differentiation of Human Induced Pluripotent Stem Cells into Endothelial Cells under Xenogeneic-free Conditions for Vascular Tissue Engineering. Acta Biomaterialia 2020, 119: 184-196. PMID: 33166710, PMCID: PMC8133308, DOI: 10.1016/j.actbio.2020.11.007.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringTissue engineeringSmall-diameter TEVGsDynamic bioreactor systemShear stressBioreactor systemCell alignmentVascular graftsXenogeneic-free conditionsEngineeringEndothelializationTEVGsApplicationsEndothelial cellsConditionsHuman induced pluripotent stem cellsAnimal-derived reagentsXenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering
Luo J, Lin Y, Shi X, Li G, Kural MH, Anderson CW, Ellis MW, Riaz M, Tellides G, Niklason LE, Qyang Y. Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2020, 119: 155-168. PMID: 33130306, PMCID: PMC8168373, DOI: 10.1016/j.actbio.2020.10.042.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringTissue-engineered vascular graftsTissue engineeringComparable mechanical strengthVascular smooth muscle cellsMechanical strengthSmooth muscle cellsPolyglycolic acid scaffoldsTechnology one stepBiodegradable polyglycolic acid (PGA) scaffoldsXenogeneic-free conditionsAnimal-derived reagentsMuscle cellsVSMC differentiationImmunodeficient mouse modelEngineeringVascular graftsOne-stepStem cellsPluripotent stem cellsMouse modelCollagen depositionComparable capacityBlood vesselsAcid scaffoldsAge-Associated Salivary MicroRNA Biomarkers for Oculopharyngeal Muscular Dystrophy.
Raz V, Kroon RHMJM, Mei H, Riaz M, Buermans H, Lassche S, Horlings C, Swart B, Kalf J, Harish P, Vissing J, Kielbasa S, van Engelen BGM. Age-Associated Salivary MicroRNA Biomarkers for Oculopharyngeal Muscular Dystrophy. International Journal Of Molecular Sciences 2020, 21 PMID: 32842713, PMCID: PMC7503697, DOI: 10.3390/ijms21176059.Peer-Reviewed Original ResearchA data-driven methodology reveals novel myofiber clusters in older human muscles.
Raz Y, van den Akker EB, Roest T, Riaz M, van de Rest O, Suchiman HED, Lakenberg N, Stassen SA, van Putten M, Feskens EJM, Reinders MJT, Goeman J, Beekman M, Raz V, Slagboom PE. A data-driven methodology reveals novel myofiber clusters in older human muscles. FASEB Journal : Official Publication Of The Federation Of American Societies For Experimental Biology 2020, 34: 5525-5537. PMID: 32141137, DOI: 10.1096/fj.201902350R.Peer-Reviewed Original ResearchTissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs
Luo J, Qin L, Zhao L, Gui L, Ellis MW, Huang Y, Kural MH, Clark JA, Ono S, Wang J, Yuan Y, Zhang SM, Cong X, Li G, Riaz M, Lopez C, Hotta A, Campbell S, Tellides G, Dardik A, Niklason LE, Qyang Y. Tissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs. Cell Stem Cell 2020, 26: 251-261.e8. PMID: 31956039, PMCID: PMC7021512, DOI: 10.1016/j.stem.2019.12.012.Peer-Reviewed Original Research
2019
Modular design of a tissue engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes
Park J, Anderson CW, Sewanan LR, Kural MH, Huang Y, Luo J, Gui L, Riaz M, Lopez CA, Ng R, Das SK, Wang J, Niklason L, Campbell SG, Qyang Y. Modular design of a tissue engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes. Acta Biomaterialia 2019, 102: 220-230. PMID: 31634626, PMCID: PMC7227659, DOI: 10.1016/j.actbio.2019.10.019.Peer-Reviewed Original ResearchConceptsSingle-ventricle cardiac defectsHuman umbilical arteryPluripotent stem cell-derived cardiomyocytesStem cell-derived cardiomyocytesFontan procedureSurgical interventionSVD patientsCell-derived cardiomyocytesFontan conduitTherapeutic potentialEngineered Heart TissueVascular conduitsCongenital disorderDesign strategyVariety of complicationsEfficient electrical conductivitySingle ventricle heartPrimary cardiac fibroblastsFontan circulationHeart failureCorrective surgeryUmbilical arteryPulmonary circulationBiodegradable materialsPulmonary systemPatient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation
Ng R, Manring H, Papoutsidakis N, Albertelli T, Tsai N, See CJ, Li X, Park J, Stevens TL, Bobbili PJ, Riaz M, Ren Y, Stoddard CE, Janssen PM, Bunch TJ, Hall SP, Lo YC, Jacoby DL, Qyang Y, Wright N, Ackermann MA, Campbell SG. Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation. JCI Insight 2019, 5 PMID: 31194698, PMCID: PMC6675562, DOI: 10.1172/jci.insight.128643.Peer-Reviewed Original ResearchTissue-Engineered Stem Cell Models of Cardiovascular Diseases
Anderson C, Luo J, Sewanan L, Kural M, Riaz M, Park J, Huang Y, Niklason L, Campbell S, Qyang Y. Tissue-Engineered Stem Cell Models of Cardiovascular Diseases. 2019, 1-18. DOI: 10.1007/978-3-030-20047-3_1.ChaptersMolecular underpinningsStem cell modelTwo-dimensional cell culture methodsComplex 3D microenvironmentThree-dimensional microenvironmentMolecular mechanismsTissue-engineered modelDisease modelingNew therapeutic strategiesCell culturesCell culture methodsCell modelPathological conditionsCulture systemDisease statesCompelling insightsTherapeutic strategiesMicroenvironmentCulture methodVivoDeacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting.
Olie CS, Riaz M, Konietzny R, Charles PD, Pinto-Fernandez A, Kiełbasa SM, Aartsma-Rus A, Goeman JJ, Kessler BM, Raz V. Deacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting. IScience 2019, 12: 318-332. PMID: 30739015, PMCID: PMC6370712, DOI: 10.1016/j.isci.2019.01.024.Peer-Reviewed Original ResearchUse of Human Cells and Heart Muscle Tissue Patches as Therapeutics for Heart Diseases
Batty L, Ellis M, Anderson C, Luo J, Riaz M, Park J, Das S, Huang Y, Jacoby D, Campbell S, Qyang Y. Use of Human Cells and Heart Muscle Tissue Patches as Therapeutics for Heart Diseases. 2019 DOI: 10.1016/b978-0-12-801238-3.65542-3.ChaptersCardiac tissue engineeringThree-dimensional tissuesCardiovascular disease epidemicRegenerative medicineTissue engineeringCardiac patchesCardiovascular healthHeart diseaseInfarcted tissueClinical useHydrogel matrixStem cellsCardiomyocytesTissue patchesRecent innovationsDisease epidemicsTissueFurther researchHuman cells
2018
The distinct transcriptomes of slow and fast adult muscles are delineated by noncoding RNAs.
Raz V, Riaz M, Tatum Z, Kielbasa SM, 't Hoen PAC. The distinct transcriptomes of slow and fast adult muscles are delineated by noncoding RNAs. FASEB Journal : Official Publication Of The Federation Of American Societies For Experimental Biology 2018, 32: 1579-1590. PMID: 29141996, DOI: 10.1096/fj.201700861R.Peer-Reviewed Original Research
2017
Proteasomal activity-based probes mark protein homeostasis in muscles.
Raz V, Raz Y, Paniagua-Soriano G, Roorda JC, Olie C, Riaz M, Florea BI. Proteasomal activity-based probes mark protein homeostasis in muscles. Journal Of Cachexia, Sarcopenia And Muscle 2017, 8: 798-807. PMID: 28675601, PMCID: PMC5659047, DOI: 10.1002/jcsm.12211.Peer-Reviewed Original ResearchAn alanine expanded PABPN1 causes increased utilization of intronic polyadenylation sites.
Abbassi-Daloii T, Yousefi S, de Klerk E, Grossouw L, Riaz M, 't Hoen PAC, Raz V. An alanine expanded PABPN1 causes increased utilization of intronic polyadenylation sites. NPJ Aging And Mechanisms Of Disease 2017, 3: 6. PMID: 28649424, PMCID: PMC5445584, DOI: 10.1038/s41514-017-0007-x.Peer-Reviewed Original Research
2016
Cytokine genes as potential biomarkers for muscle weakness in OPMD.
Riaz M, Raz Y, van der Slujis B, Dickson G, van Engelen B, Vissing J, Raz V. Cytokine genes as potential biomarkers for muscle weakness in OPMD. Human Molecular Genetics 2016, 25: 4282-4287. PMID: 27506982, DOI: 10.1093/hmg/ddw259.Peer-Reviewed Original Research