1998
Fractionation of Rat Hepatocyte Subpopulations with Varying Metabolic Potential, Proliferative Capacity, and Retroviral Gene Transfer Efficiency
Rajvanshi P, Liu D, Ott M, Gagandeep S, Schilsky M, Gupta S. Fractionation of Rat Hepatocyte Subpopulations with Varying Metabolic Potential, Proliferative Capacity, and Retroviral Gene Transfer Efficiency. Experimental Cell Research 1998, 244: 405-419. PMID: 9806791, DOI: 10.1006/excr.1998.4223.Peer-Reviewed Original ResearchConceptsH4 cellsGene expressionRetroviral gene transfer efficiencyHepatocyte subpopulationsMetabolic potentialCellular DNA synthesisLiver growth controlComplex cytoplasmRetroviral gene transferGrowth controlHepatic gene expressionGene transferHepatocyte growth factorBiosynthetic rateCell proliferationDNA synthesisGene transfer efficiencyViral receptorsProliferative capacityPolyploid hepatocytesGrowth factorCytoplasmCellsBiological differencesCytoplasmic ratio
1995
Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin-UDP-glucuronosyltransferase gene transfer
Fox I, Chowdhury N, Gupta S, Kondapalli R, Schilsky M, Stockert R, Chowdhury J. Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin-UDP-glucuronosyltransferase gene transfer. Hepatology 1995, 21: 837-846. DOI: 10.1016/0270-9139(95)90539-1.Peer-Reviewed Original ResearchLarge T antigenSV40 large T antigenT antigenGunn rat hepatocytesImmortalized cellsMutant SV40 large T antigenPrimary hepatocytesRecombinant Moloney murine leukemia virusGene transfer vectorsMoloney murine leukemia virusTransfer vectorsGlutathione S-transferaseMurine leukemia virusCorresponding mRNAExpression of albuminT promoterRat hepatocytesConditional immortalizationASGRGene transferCell growthBilirubin UGTDNA synthesisHepatocyte clonesSynthesized DNAConditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin‐UDP‐glucuronosyltransferase gene transfer
Fox I, Chowdhury N, Gupta S, Kondapalli R, Schilsky M, Stockert R, Chowdhury J. Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin‐UDP‐glucuronosyltransferase gene transfer. Hepatology 1995, 21: 837-846. PMID: 7875682, DOI: 10.1002/hep.1840210334.Peer-Reviewed Original ResearchConceptsLarge T antigenSV40 large T antigenGene transfer vectorsT antigenGunn rat hepatocytesMutant SV40 large T antigenImmortalized cellsPrimary hepatocytesTransfer vectorsRecombinant Moloney murine leukemia virusMoloney murine leukemia virusMurine leukemia virusCorresponding mRNAExpression of albuminT promoterRat hepatocytesConditional immortalizationASGRGene transferCell growthViral vectorsBilirubin UGTDNA synthesisUDP-glucuronosyltransferase deficiencyHepatocyte clones