2015
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes
Weerachayaphorn J, Amaya MJ, Spirli C, Chansela P, Mitchell-Richards KA, Ananthanarayanan M, Nathanson MH. Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes. Gastroenterology 2015, 149: 211-222.e10. PMID: 25796361, PMCID: PMC4478166, DOI: 10.1053/j.gastro.2015.03.014.Peer-Reviewed Original ResearchConceptsBile ductBile duct unitsCholestatic disordersOxidative stressCalcium signalingNuclear factorMouse cholangiocytesDuct unitsReduced calcium signalingIntrahepatic bile ductsLevels of Nrf2Cholangiocyte cellsKnockdown of Nrf2Activation of Nrf2Intracellular calcium release channelsTranscription factor Nrf2Binding of Nrf2Calcium release channelPolymerase chain reaction analysisBiliary diseaseTrisphosphate receptorControl ratsLiver disordersBicarbonate secretionChain reaction analysis
2014
Apical Localization of Inositol 1,4,5-Trisphosphate Receptors Is Independent of Extended Synaptotagmins in Hepatocytes
Amaya MJ, Oliveira AG, Schroeder LK, Allgeyer ES, Bewersdorf J, Nathanson MH. Apical Localization of Inositol 1,4,5-Trisphosphate Receptors Is Independent of Extended Synaptotagmins in Hepatocytes. PLOS ONE 2014, 9: e114043. PMID: 25437447, PMCID: PMC4250053, DOI: 10.1371/journal.pone.0114043.Peer-Reviewed Original Research
2013
The insulin receptor translocates to the nucleus to regulate cell proliferation in liver
Amaya MJ, Oliveira AG, Guimarães ES, Casteluber MC, Carvalho SM, Andrade LM, Pinto MC, Mennone A, Oliveira CA, Resende RR, Menezes GB, Nathanson MH, Leite MF. The insulin receptor translocates to the nucleus to regulate cell proliferation in liver. Hepatology 2013, 59: 274-283. PMID: 23839970, PMCID: PMC3823683, DOI: 10.1002/hep.26609.Peer-Reviewed Original ResearchConceptsInsulin's metabolic effectsInsulin's mitogenic effectsInsulin receptorCell proliferationMitogenic effectMetabolic effectsInsulin-induced increaseFormation of inositolHepatic glucose metabolismInsulin's abilityFormation of InsP3Potential targetPathwayTherapeutic modulationGlucose metabolismProliferationInsP3Partial hepatectomyHepatic mitogenLiver growthLiver regenerationNucleusClathrinReceptorsLiver
2001
Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia
Dranoff J, Masyuk A, Kruglov E, LaRusso N, Nathanson M. Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. AJP Gastrointestinal And Liver Physiology 2001, 281: g1059-g1067. PMID: 11557527, DOI: 10.1152/ajpgi.2001.281.4.g1059.Peer-Reviewed Original ResearchConceptsP2Y receptor subtypesP2Y receptorsReceptor subtypesIntrahepatic bile duct unitsATP receptorsBile duct epitheliumMultiple P2Y receptor subtypesRat bile duct epitheliaBile duct unitsImportant regulatorLuminal ATPLuminal perfusionMolecular subtypesBile secretionDuct epitheliumBasolateral additionExtracellular nucleotidesDuctular secretionSubtypesCytosolic Ca2Duct unitsRT-PCRCholangiocytesReceptorsSecretionBile duct epithelia regulate biliary bicarbonate excretion in normal rat liver
Hirata K, Nathanson M. Bile duct epithelia regulate biliary bicarbonate excretion in normal rat liver. Gastroenterology 2001, 121: 396-406. PMID: 11487549, DOI: 10.1053/gast.2001.26280.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAcetylcholineAngiogenesis InhibitorsAnimalsAnti-Inflammatory Agents, Non-SteroidalBicarbonatesBileBile DuctsBiological TransportCalciumCyclic AMPCyclosporineDose-Response Relationship, DrugEnzyme InhibitorsEpithelial CellsGlucagonHepatic ArteryHepatic VeinsIn Vitro TechniquesLiverMaleNitrobenzoatesOrtho-AminobenzoatesRatsRats, Sprague-DawleySecretinVasoconstrictor AgentsVasodilator AgentsVasopressinsConceptsBiliary bicarbonate excretionEffect of secretinBicarbonate excretionHepatic arteryBiliary bicarbonateNormal rat liverDiphenylamine-2-carboxylic acidBile ductPortal veinRat liverEffects of acetylcholineBile duct epitheliumDose-dependent fashionFormation of bileBile flowBlood supplyBicarbonate exchangeDuct epitheliumAcetylcholineArteryAbstractTextExcretionCyclosporin A.LiverCholangiocytesProinflammatory Cytokines Inhibit Secretion in Rat Bile Duct Epithelium
Spirlı̀ C, Nathanson M, Fiorotto R, Duner E, Denson L, Sanz J, Di Virgilio F, Okolicsanyi L, Casagrande F, Strazzabosco M. Proinflammatory Cytokines Inhibit Secretion in Rat Bile Duct Epithelium. Gastroenterology 2001, 121: 156-169. PMID: 11438505, DOI: 10.1053/gast.2001.25516.Peer-Reviewed Original ResearchConceptsProinflammatory cytokinesFluorescein-labeled dextranIL-1Interferon gammaCAMP-dependent fluid secretionCystic fibrosis transmembrane conductance regulatorBile duct epitheliumRat bile duct epitheliaTumor necrosis factorCyclic adenosine monophosphate levelsSecretin receptorAdenosine monophosphate levelsBile duct unitsDuctular cholestasisPortal inflammationCholestatic disordersIL-6Inflammatory cytokinesTNF-alphaBiliary epitheliumNecrosis factorCellular cyclic adenosine monophosphate (cAMP) levelsDuct epitheliumPurinergic agonistsSR expression
2000
A Primitive ATP Receptor from the Little Skate Raja erinacea *
Dranoff J, O'Neill A, Franco A, Cai S, Connolly G, Ballatori N, Boyer J, Nathanson M. A Primitive ATP Receptor from the Little Skate Raja erinacea *. Journal Of Biological Chemistry 2000, 275: 30701-30706. PMID: 10900200, DOI: 10.1074/jbc.m003366200.Peer-Reviewed Original ResearchConceptsLittle skate Raja erinaceaSkate Raja erinaceaDegenerate oligonucleotide probeRaja erinaceaP2Y receptor pharmacologySingle polymerase chain reaction productXenopus oocyte expression systemLiver cDNA libraryATP receptorsHigher organismsEvolutionary forerunnerCommon ancestorOocyte expression systemPhylogenetic analysisP2Y receptorsCDNA clonesPrimitive organismsCDNA libraryGene productsSubstrate preferenceMolecular basisExpression systemNucleotide specificityMammalian tissuesRange of nucleotidesIt's swell to have ATP in the liver
Dranoff J, Nathanson M. It's swell to have ATP in the liver. Journal Of Hepatology 2000, 33: 323-325. PMID: 10952251, DOI: 10.1016/s0168-8278(00)80374-8.Peer-Reviewed Original Research
1999
Short‐term regulation of bile acid uptake by microfilament‐dependent translocation of rat ntcp to the plasma membrane
Dranoff J, McClure M, Burgstahler A, Denson L, Crawford A, Crawford J, Karpen S, Nathanson M. Short‐term regulation of bile acid uptake by microfilament‐dependent translocation of rat ntcp to the plasma membrane. Hepatology 1999, 30: 223-229. PMID: 10385660, DOI: 10.1002/hep.510300136.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsBase SequenceBile Acids and SaltsBiological TransportCarcinoma, HepatocellularCarrier ProteinsCell MembraneCyclic AMPCytochalasin DGreen Fluorescent ProteinsHumansKineticsLiver NeoplasmsLuminescent ProteinsMembrane Transport ProteinsModels, MolecularMolecular Sequence DataOpen Reading FramesOrganic Anion Transporters, Sodium-DependentProtein Structure, SecondaryRatsRecombinant Fusion ProteinsSymportersTaurocholic AcidTransfectionTritiumTumor Cells, CulturedConceptsPlasma membraneGreen fluorescence proteinGFP fluorescenceNTCP-GFPPlasma membrane fluorescenceMembrane fluorescenceRat NtcpInhibitor of microfilamentsSecond messenger adenosinePrimary transporterFluorescence proteinNocodazole removalCytochalasin DTransporter activityShort-term regulationAcid uptakeConfocal video microscopyMicrotubule inhibitorsHepG2 cellsTaurocholate cotransportMembraneProteinMicrofilamentsCellsVideo microscopyRegulation of bile acid transport: Beyond molecular cloning
Dranoff J, Nathanson M. Regulation of bile acid transport: Beyond molecular cloning. Hepatology 1999, 29: 1912-1913. PMID: 10347140, DOI: 10.1002/hep.510290643.Peer-Reviewed Original ResearchCommunication via gap junctions modulates bile secretion in the isolated perfused rat liver
Nathanson M, Rios–Velez L, Burgstahler A, Mennone A. Communication via gap junctions modulates bile secretion in the isolated perfused rat liver. Gastroenterology 1999, 116: 1176-1183. PMID: 10220510, DOI: 10.1016/s0016-5085(99)70021-1.Peer-Reviewed Original Research
1998
Type III InsP3 receptor channel stays open in the presence of increased calcium
Hagar R, Burgstahler A, Nathanson M, Ehrlich B. Type III InsP3 receptor channel stays open in the presence of increased calcium. Nature 1998, 396: 81-84. PMID: 9817204, PMCID: PMC2825878, DOI: 10.1038/23954.Peer-Reviewed Original ResearchNovel paracrine signaling mechanism in the ocular ciliary epithelium
Hirata K, Nathanson M, Sears M. Novel paracrine signaling mechanism in the ocular ciliary epithelium. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 8381-8386. PMID: 9653195, PMCID: PMC20984, DOI: 10.1073/pnas.95.14.8381.Peer-Reviewed Original ResearchConceptsNonpigmented cell layerCiliary epithelial bilayerAlpha1-adrenergic antagonist prazosinBeta-adrenergic antagonist propranololPigmented cell layerGap junction blocker octanolBeta-adrenergic agonist isoproterenolCell layerEpithelial bilayerEndogenous adenylyl cyclaseGap junctionsCell spreadOcular ciliary epitheliumAntagonist prazosinAqueous humor secretionAntagonist propranololCiliary bodyAgonist isoproterenolParacrine pathwaysAdrenergic stimulationNovel paracrineCytosolic Ca2Lucifer YellowCiliary epitheliumAdenylyl cyclaseTelenzepine-sensitive muscarinic receptors on rat pancreatic acinar cells
Schmid S, Modlin I, Tang L, Stoch A, Rhee S, Nathanson M, Scheele G, Gorelick F. Telenzepine-sensitive muscarinic receptors on rat pancreatic acinar cells. American Journal Of Physiology 1998, 274: g734-g741. PMID: 9575856, DOI: 10.1152/ajpgi.1998.274.4.g734.Peer-Reviewed Original ResearchConceptsPancreatic acinar cellsAmylase secretionRat pancreatic acinar cellsMuscarinic receptorsAcinar cellsDifferent muscarinic receptor antagonistsM2 antagonist methoctramineMaximal amylase secretionMuscarinic receptor antagonistIsolated rat pancreatic aciniRat pancreatic aciniAntagonist methoctramineM3 subtypeM1 antagonistMuscarinic subtypesReceptor antagonistZymogen processingAntagonist sensitivityPancreatic aciniSecretionAntagonistSubtypesPotent inhibitorReceptorsDistinct patternsCoordination of calcium waves among hepatocytes: Teamwork gets the job done
Burgstahler A, Nathanson M. Coordination of calcium waves among hepatocytes: Teamwork gets the job done. Hepatology 1998, 27: 634-635. PMID: 9462668, DOI: 10.1002/hep.510270244.Peer-Reviewed Original Research
1997
Mechanism of long-range Ca2+ signalling in the nucleus of isolated rat hepatocytes
Jeffrey L, BURGSTAHLER A, NATHANSON M. Mechanism of long-range Ca2+ signalling in the nucleus of isolated rat hepatocytes. Biochemical Journal 1997, 326: 491-495. PMID: 9291123, PMCID: PMC1218696, DOI: 10.1042/bj3260491.Peer-Reviewed Original Research
1994
Effects of protein kinase C and cytosolic Ca2+ on exocytosis in the isolated perfused rat liver
Bruck R, Nathanson M, Roelofsen H, Boyer J. Effects of protein kinase C and cytosolic Ca2+ on exocytosis in the isolated perfused rat liver. Hepatology 1994, 20: 1032-1040. PMID: 7927205, DOI: 10.1002/hep.1840200436.Peer-Reviewed Original ResearchConceptsProtein kinase CKinase CApical exocytosisRegulation of exocytosisProtein kinase C inhibitor HHorseradish peroxidase concentrationCytosolic Ca2ExocytosisCell typesInhibitor HProtein kinase C activator phorbol dibutyrateHorseradish peroxidase excretionPhorbol dibutyrateCa2Rat liverRelative importanceNet excretionPeroxidase concentrationsHepatocytes
1993
Effects of tauroursodeoxycholic acid on cytosolic Ca2+ signals in isolated rat hepatocytes
Beuers U, Nathanson M, Boyer J. Effects of tauroursodeoxycholic acid on cytosolic Ca2+ signals in isolated rat hepatocytes. Gastroenterology 1993, 104: 604-612. PMID: 8425704, DOI: 10.1016/0016-5085(93)90433-d.Peer-Reviewed Original ResearchConceptsEffect of TUDCATauroursodeoxycholic acidAdministration of TUDCAIP3-independent mechanismCytosolic free calciumIP3-sensitive Ca2Trihydroxy bile acidsCholestatic disordersHepatocyte secretionTreatment periodExtracellular Ca2Bile acidsTaurolithocholic acidMumol/Rat hepatocytesFree calciumBasal levelsTransient increaseCytosolic Ca2Levels of inositolFree mediumPhysiological concentrationsIsolated rat hepatocytesHepatocytesFourfold increase
1992
Immunocytochemical expression and localization of protein kinase C in bovine aortic endothelial cells
Rosales O, Isales C, Nathanson M, Sumpio B. Immunocytochemical expression and localization of protein kinase C in bovine aortic endothelial cells. Biochemical And Biophysical Research Communications 1992, 189: 40-46. PMID: 1449492, DOI: 10.1016/0006-291x(92)91522-r.Peer-Reviewed Original ResearchConceptsSerum-deprived mediumPKC activityPhorbol ester-induced increaseAortic endothelial cellsBovine aortic endothelial cellsBeta stainingSDM groupTotal PKC activityImmunocytochemical expressionBeta PKC isoformsEndothelial cellsParticulate fractionFBS groupProtein kinase CCalphostin CPKC inhibitorPhorbol esterConfocal microscopyDiffuse cytoplasmic localizationCytosolic fractionGamma-PKCKinase CPKC isoformsCytoplasmic localizationCellsEffects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver
Nathanson M, Gautam A, Bruck R, Isales C, Boyer J. Effects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver. Hepatology 1992, 15: 107-116. PMID: 1727785, DOI: 10.1002/hep.1840150119.Peer-Reviewed Original ResearchConceptsBile flowPerfusion pressureBile secretionCytosolic Ca2Rat liverProtein kinase CIonophore-induced increaseBromo-A23187Hepatic vasoconstrictionVasodilator papaverineProtein kinase C inhibitor HExtracellular Ca2Kinase CSecretionLiverVasopressinAgonist vasopressinBenzohydroquinoneEffect of Ca2HepatocytesActivate protein kinase CInhibitor HTwo-thirdsIsolated hepatocytesSensitive photoprotein aequorin