2023
Multiple Subthreshold GPCR Signals Combined by the G-Proteins Gαq and Gαs Activate the Caenorhabditis elegans Egg-Laying Muscles
Olson A, Butt A, Christie N, Shelar A, Koelle M. Multiple Subthreshold GPCR Signals Combined by the G-Proteins Gαq and Gαs Activate the Caenorhabditis elegans Egg-Laying Muscles. Journal Of Neuroscience 2023, 43: 3789-3806. PMID: 37055179, PMCID: PMC10219013, DOI: 10.1523/jneurosci.2301-22.2023.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsMultiple G protein-coupled receptorsMuscle cellsMuscle activitySerotonin G protein-coupled receptorsDesigner G protein-coupled receptorsIntact animalsG proteinsEndogenous G protein-coupled receptorsIndividual G protein-coupled receptorsGPCR signalsCalcium activityEgg-laying musclesSerotoninIndividual neuronsDouble knockoutNeuronsBehavioral outcomesMuscleMost cellsG protein GαqCellsSubthreshold signalEgg-laying systemSER-1
2008
Regulation of Serotonin Biosynthesis by the G Proteins Gαo and Gαq Controls Serotonin Signaling in Caenorhabditis elegans
Tanis JE, Moresco JJ, Lindquist RA, Koelle MR. Regulation of Serotonin Biosynthesis by the G Proteins Gαo and Gαq Controls Serotonin Signaling in Caenorhabditis elegans. Genetics 2008, 178: 157-169. PMID: 18202365, PMCID: PMC2206068, DOI: 10.1534/genetics.107.079780.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCaenorhabditis elegansCaenorhabditis elegans ProteinsGene Expression Regulation, EnzymologicGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11Motor NeuronsMusclesOrgan SpecificityOvipositionPromoter Regions, GeneticSerotoninSignal TransductionSynapsesTryptophan HydroxylaseConceptsEgg-laying behaviorCaenorhabditis elegansG proteinsEgg-laying systemHSN motor neuronsSerotonin biosynthesisG protein GαoLevel of transcriptionEpistasis experimentsTPH-1 expressionRate-limiting enzymeGene dosageSpecific genesGalphaoGalphaqTranscriptionBiosynthesisMotor neuronsElegansTPH-1Neurotransmitter releaseGenesMultiple signalsProteinEggs
2003
Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior
Bany IA, Dong MQ, Koelle MR. Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior. Journal Of Neuroscience 2003, 23: 8060-8069. PMID: 12954868, PMCID: PMC6740490, DOI: 10.1523/jneurosci.23-22-08060.2003.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAcetylcholinesteraseAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsCholinesterase InhibitorsDNA, ComplementaryGTP-Binding ProteinsHomeodomain ProteinsInhibition, PsychologicalMutationNeuronsNuclear ProteinsOvipositionPhenotypeReceptors, CholinergicSignal TransductionSynapsesConceptsHermaphrodite-specific neuronsEgg-laying behaviorG proteinsG-protein signaling genesEgg-laying defectsEgg-laying musclesEgg-laying systemAnalysis of mutantsInhibition of eggSerotonergic Hermaphrodite Specific NeuronsUnc-4Caenorhabditis elegansUnc-17Signaling GenesThird cell typeActivation of eggsMorphological defectsCha-1MutantsCell typesCellular basisNeurotransmitter releaseGenesEggsPartial defect