1999
Thresholds for cellular disruption and activation of the stress response in renal epithelia
van Why S, Kim S, Geibel J, Seebach F, Kashgarian M, Siegel N. Thresholds for cellular disruption and activation of the stress response in renal epithelia. American Journal Of Physiology 1999, 277: f227-f234. PMID: 10444577, DOI: 10.1152/ajprenal.1999.277.2.f227.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCysteine EndopeptidasesDetergentsDifferential ThresholdDNA-Binding ProteinsEpithelial CellsHeat Shock Transcription FactorsHeat-Shock ProteinsIntracellular MembranesKidneyL-Lactate DehydrogenaseLLC-PK1 CellsMultienzyme ComplexesOctoxynolProteasome Endopeptidase ComplexSodium-Potassium-Exchanging ATPaseSolubilityStress, PhysiologicalSwineTranscription FactorsConceptsATP depletionRenal epitheliumLactate dehydrogenase releaseCellular ATPReduction of ATPRenal ischemiaIntracellular calciumActivation of HSF1Heat shock transcription factorDehydrogenase releaseControl ATPStress responseControl levelsProgressive accumulationProteasome inhibitionAdaptive inductionHSF activationRapid fallActivationEpitheliumIncremental increaseCellular disruptionResponseATPIschemia
1998
Ischemic Pretreatment Increases Cytoskeletal Association of 25 kD and 70 kD Heat-Shock Proteins and of Na,K-ATPase in Rat Renal Cortex after Repeat Ischemia † 1786
Aufricht C, Bidmon B, Herkner K, Siegel N, Kashgarian M, Van Why S. Ischemic Pretreatment Increases Cytoskeletal Association of 25 kD and 70 kD Heat-Shock Proteins and of Na,K-ATPase in Rat Renal Cortex after Repeat Ischemia † 1786. Pediatric Research 1998, 43: 304-304. DOI: 10.1203/00006450-199804001-01808.Peer-Reviewed Original ResearchHeat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia
Aufricht C, Ardito T, Thulin G, Kashgarian M, Siegel N, Van Why S. Heat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia. American Journal Of Physiology 1998, 274: f215-f222. PMID: 9458842, DOI: 10.1152/ajprenal.1998.274.1.f215.Peer-Reviewed Original ResearchConceptsHSP-25Renal ischemiaHeat shock protein 25Ischemic renal injuryH reflowRat renal cortexControl proximal tubulesRenal injuryPostischemic recoveryRenal cortexIschemiaProximal tubulesIntracytoplasmic accumulationPeak levelsHeat shock proteinsProtein 25Actin stainingInsoluble cytoskeletal fractionPunctate accumulationsCytoskeletal disruptionSmall heat shock proteinsRegulatory roleInductionIntracellular distributionCytoskeletal fraction
1992
Induction and intracellular localization of HSP-72 after renal ischemia
Van Why S, Hildebrandt F, Ardito T, Mann A, Siegel N, Kashgarian M. Induction and intracellular localization of HSP-72 after renal ischemia. American Journal Of Physiology 1992, 263: f769-f775. PMID: 1443167, DOI: 10.1152/ajprenal.1992.263.5.f769.Peer-Reviewed Original ResearchConceptsHSP 72Renal ischemiaHeat shock proteinsIschemic renal injuryMin of reperfusionProtein elaborationHSP mRNA expressionHSP-70 mRNARenal injuryIschemic injuryTransient ischemiaProximal tubulesIschemiaRat kidneyMRNA expressionIntracellular localizationMRNA inductionInjuryTubule morphologyImmunofluorescent localizationShock proteinsVesicular patternCellular recoveryHSP proteinsCellular remodeling
1989
Protection of the kidney against ischemic injury by inhibition of 5'-nucleotidase
Van Waarde A, Stromski M, Thulin G, Gaudio K, Kashgarian M, Shulman R, Siegel N. Protection of the kidney against ischemic injury by inhibition of 5'-nucleotidase. American Journal Of Physiology 1989, 256: f298-f305. PMID: 2537026, DOI: 10.1152/ajprenal.1989.256.2.f298.Peer-Reviewed Original ResearchConceptsRenal ischemic insultResidual nucleotide poolMin of ischemiaRenal ATP contentFunctional recoveryIschemic injuryIschemic insultKidney functionRenal ischemiaIntramuscular injectionUntreated ratsRapid initial recoveryATP restorationRat kidneyRenal ATPIschemiaInitial recoveryKidneyRatsATP contentInsultFunctional consequencesNucleotide poolMagnetic resonanceAMPCP
1988
Metabolic and functional consequences of inhibiting adenosine deaminase during renal ischemia in rats.
Stromski M, van Waarde A, Avison M, Thulin G, Gaudio K, Kashgarian M, Shulman R, Siegel N. Metabolic and functional consequences of inhibiting adenosine deaminase during renal ischemia in rats. Journal Of Clinical Investigation 1988, 82: 1694-1699. PMID: 3263396, PMCID: PMC442739, DOI: 10.1172/jci113782.Peer-Reviewed Original ResearchConceptsPostischemic restorationFunctional recoveryBilateral renal artery occlusionAdenosine deaminaseResidual nucleotide poolRenal artery occlusionEnd of ischemiaMin of ischemiaMin of reflowArtery occlusionAdenine nucleotide poolPostischemic recoveryRenal ischemiaNucleotide poolIschemia resultsUntreated animalsDegradation of ATPIschemiaBasal levelsRenal ATPATP levelsInitial recoveryRatsInsultFunctional consequencesBeneficial effect of thyroxin in the treatment of ischemic acute renal failure
Sutter P, Thulin G, Stromski M, Ardito T, Gaudio K, Kashgarian M, Siegel N. Beneficial effect of thyroxin in the treatment of ischemic acute renal failure. Pediatric Nephrology 1988, 2: 1-7. PMID: 2856365, DOI: 10.1007/bf00870370.Peer-Reviewed Original ResearchConceptsAcute renal failureIschemic acute renal failureRenal failureIschemic controlsNormal salineSaline-treated ischemic controlsBeneficial effectsBetter kidney functionDose of T4Min of reflowT4-treated ratsEffect of thyroxinRenal functionKidney functionNS animalsRenal ischemiaInulin clearanceSaline controlsDay 3Day 7IschemiaT4 animalsRenal ATPCellular mechanismsSignificant increase
1984
HEMODYNAMIC EFFECT OF POST-ISCHEMIC ATP-MGCL2
Gaudio K, Thulin G, Kashgarian M, Siegel N. HEMODYNAMIC EFFECT OF POST-ISCHEMIC ATP-MGCL2. Pediatric Research 1984, 18: 362-362. DOI: 10.1203/00006450-198404001-01613.Peer-Reviewed Original ResearchRenal blood flowHemodynamic effectsATP-MgCl2Normal salineBlood flowInitial hemodynamic effectsAcute renal failureInfusion of dopamineRenal failureRenal ischemiaPostischemic infusionInulin clearanceVasoactive compoundsTubular cellsSalutary effectsCINInfusionHrDopamineCellular metabolismIschemiaInjuryRatsSalineClearance
1980
ISCHEMIC ACUTE RENAL FAILURE: DELAYED INFUSION OF ATP-MGCL2
Siegel N, Taylor M, Gaudio K, Chaudry I, Kashgarian M. ISCHEMIC ACUTE RENAL FAILURE: DELAYED INFUSION OF ATP-MGCL2. Pediatric Research 1980, 14: 1017-1017. DOI: 10.1203/00006450-198008000-00262.Peer-Reviewed Original ResearchMinutes of ischemiaATP-MgCl2Normal salineIschemic insultBilateral renal artery occlusionModerate ischemic insultRenal artery occlusionInfusion of ATPDelayed infusionRenal injuryArtery occlusionIschemic injuryRenal ischemiaInitial injuryInitial insultInulin clearanceInjuryIschemiaInfusionInsultCINAnimalsMinutesHoursPrevious studies