1999
Thresholds for cellular disruption and activation of the stress response in renal epithelia
van Why S, Kim S, Geibel J, Seebach F, Kashgarian M, Siegel N. Thresholds for cellular disruption and activation of the stress response in renal epithelia. American Journal Of Physiology 1999, 277: f227-f234. PMID: 10444577, DOI: 10.1152/ajprenal.1999.277.2.f227.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCysteine EndopeptidasesDetergentsDifferential ThresholdDNA-Binding ProteinsEpithelial CellsHeat Shock Transcription FactorsHeat-Shock ProteinsIntracellular MembranesKidneyL-Lactate DehydrogenaseLLC-PK1 CellsMultienzyme ComplexesOctoxynolProteasome Endopeptidase ComplexSodium-Potassium-Exchanging ATPaseSolubilityStress, PhysiologicalSwineTranscription FactorsConceptsATP depletionRenal epitheliumLactate dehydrogenase releaseCellular ATPReduction of ATPRenal ischemiaIntracellular calciumActivation of HSF1Heat shock transcription factorDehydrogenase releaseControl ATPStress responseControl levelsProgressive accumulationProteasome inhibitionAdaptive inductionHSF activationRapid fallActivationEpitheliumIncremental increaseCellular disruptionResponseATPIschemia
1994
Activation of heat-shock transcription factor by graded reductions in renal ATP, in vivo, in the rat.
Van Why S, Mann A, Thulin G, Zhu X, Kashgarian M, Siegel N. Activation of heat-shock transcription factor by graded reductions in renal ATP, in vivo, in the rat. Journal Of Clinical Investigation 1994, 94: 1518-1523. PMID: 7929828, PMCID: PMC295298, DOI: 10.1172/jci117492.Peer-Reviewed Original ResearchConceptsRenal cortexHsp-70 mRNA inductionCortical ATP levelsRenal ischemia resultsPartial aortic occlusionActivation of HSFATP levelsCellular ATPHSF activationHSP-70 mRNAReduction of ATPAortic occlusionPreocclusion levelsProfound fallTotal occlusionIschemia resultsPreocclusion valuesHeat shock transcription factorLow-level expressionMetabolic consequencesVivo 31P NMR spectroscopyControl valuesHSP-70Vigorous responseRenal ATP