2016
Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Długosz E, Wędrychowicz H. Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection. Experimental Parasitology 2016, 163: 1-7. PMID: 26795262, DOI: 10.1016/j.exppara.2016.01.006.Peer-Reviewed Original ResearchConceptsA. ceylanicum infectionAncylostoma ceylanicum infectionHookworm speciesWorm burden reductionsSyrian golden hamstersProtective immunityDNA vaccineGut mucosaGastrointestinal tractIntestinal nematodesGolden hamstersBurden reductionAdult wormsHemoglobin digestionEgg countsImmunizationVaccineInfectionHamstersMetalloproteasesMucosaHookwormBloodTractImmunity
2014
Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum
Fairfax KC, Harrison LM, Cappello M. Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum. Molecular And Biochemical Parasitology 2014, 198: 37-44. PMID: 25481749, PMCID: PMC4354846, DOI: 10.1016/j.molbiopara.2014.11.005.Peer-Reviewed Original ResearchConceptsAmino acid sequenceFatty acids de novoShares sequence identityExcretory/secretory proteinsPseudocoelomic spaceMolecular cloningCis-parinaric acidPartial cDNASequence identityAcid sequenceFatty acidsSimilar proteinsSecretory proteinsHookworm biologyRecombinant subunitsSoluble adult worm extractProteinAntigens/allergensSubunits
2013
Peroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A
Nguyen JB, Pool CD, Wong CY, Treger RS, Williams DL, Cappello M, Lea WA, Simeonov A, Vermeire JJ, Modis Y. Peroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A. Cell Chemical Biology 2013, 20: 991-1001. PMID: 23891152, PMCID: PMC3755041, DOI: 10.1016/j.chembiol.2013.06.011.Peer-Reviewed Original Research
2011
Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins
Kucera K, Harrison LM, Cappello M, Modis Y. Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins. Journal Of Molecular Biology 2011, 408: 9-17. PMID: 21352830, PMCID: PMC3070796, DOI: 10.1016/j.jmb.2011.02.033.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAncylostomatoideaAncylostomiasisAnimalsComplement System ProteinsHelminth ProteinsHumansMatrix MetalloproteinasesMolecular Sequence DataNerve Growth FactorsNetrin-1Protein ConformationProtein FoldingSequence Homology, Amino AcidTissue Inhibitor of MetalloproteinasesTumor Suppressor ProteinsConceptsMatrix metalloproteasesHost immune responseComplement factor C3Nematode proteinsProtein 2Complement-mediated cell lysisHookworm anaemiaMucosal vaccinesImmune responseUseful model organismFactor C3Decoy receptorDisease pathogenesisTissue inhibitorIntestinal attachmentNovel familyAncylostoma ceylanicumImmunoreactive moleculesAdult wormsGlobal healthModel organismsHookwormSequence homologyÅ resolutionHuman parasites
2008
A Leishmania Ortholog of Macrophage Migration Inhibitory Factor Modulates Host Macrophage Responses
Kamir D, Zierow S, Leng L, Cho Y, Diaz Y, Griffith J, McDonald C, Merk M, Mitchell RA, Trent J, Chen Y, Kwong YK, Xiong H, Vermeire J, Cappello M, McMahon-Pratt D, Walker J, Bernhagen J, Lolis E, Bucala R. A Leishmania Ortholog of Macrophage Migration Inhibitory Factor Modulates Host Macrophage Responses. The Journal Of Immunology 2008, 180: 8250-8261. PMID: 18523291, PMCID: PMC2668862, DOI: 10.4049/jimmunol.180.12.8250.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, Differentiation, B-LymphocyteApoptosis Regulatory ProteinsCell LineCells, CulturedCrystallography, X-RayHistocompatibility Antigens Class IIHumansIntramolecular OxidoreductasesLeishmania majorMacrophage Migration-Inhibitory FactorsMacrophages, PeritonealMiceMice, Inbred BALB CMice, Inbred C3HMice, KnockoutMolecular Sequence DataRecombinant ProteinsStructural Homology, ProteinConceptsMacrophage migration inhibitory factorCD74-dependent mannerMigration inhibitory factorImmune defense mechanismsHuman macrophage migration inhibitory factorSmall molecule antagonistsActivation-induced apoptosisHost macrophage responseMIF receptorMIF proteinImmune destructionObligate intracellular parasitesMAP kinase activationERK1/2 MAP kinase activationInhibitory factorMacrophage responseLeishmania majorIntracellular parasitesHigh-resolution X-ray crystal structuresSpecies-specific inhibitionMacrophagesSignificant structural homologyKinase activationDefense mechanismsMammalian counterparts
2007
Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*
Cho Y, Jones BF, Vermeire JJ, Leng L, DiFedele L, Harrison LM, Xiong H, Kwong YK, Chen Y, Bucala R, Lolis E, Cappello M. Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*. Journal Of Biological Chemistry 2007, 282: 23447-23456. PMID: 17567581, PMCID: PMC3707627, DOI: 10.1074/jbc.m702950200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntigens, Differentiation, B-LymphocyteCricetinaeCytokinesHistocompatibility Antigens Class IIHumansIntestinal MucosaMacrophage Migration-Inhibitory FactorsMesocricetusMolecular Sequence DataProtein BindingRabbitsRecombinant ProteinsSequence Homology, Amino AcidConceptsMacrophage migration inhibitory factorMIF receptor CD74Migration inhibitory factorReceptor CD74Inhibitory factorHost immune responseHuman macrophage migration inhibitory factorMIF proteinHookworm Ancylostoma ceylanicumImmune responseDisease pathogenesisHuman cytokinesMacrophage migrationAncylostoma ceylanicumMajor causeWorm survivalParasite survivalISO-1Significant differencesTautomerase activityHookworm proteinsCytokinesCD74HookwormRelative bioactivity
2006
Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology
Brown AC, Harrison LM, Kapulkin W, Jones BF, Sinha A, Savage A, Villalon N, Cappello M. Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology. Molecular And Biochemical Parasitology 2006, 151: 141-147. PMID: 17129620, PMCID: PMC1831819, DOI: 10.1016/j.molbiopara.2006.10.017.Peer-Reviewed Original ResearchConceptsC-type lectinRecombinant proteinsAmino acid mature proteinDrosophila S2 cellsPutative C-type lectinReproductive physiologySecretory signal sequenceEssential cell functionsSoluble protein extractsEgg cell membraneS2 cellsMature proteinCDNA correspondingProtein familyCDNA correspondsSignal sequenceMolecular cloningSperm proteinsRelated proteinsReverse transcription-PCRProtein extractsCell membraneProteinTranscription-PCRCell function
2004
Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms
Bungiro RD, Solis CV, Harrison LM, Cappello M. Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms. Infection And Immunity 2004, 72: 2203-2213. PMID: 15039344, PMCID: PMC375217, DOI: 10.1128/iai.72.4.2203-2213.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, OralAmino Acid SequenceAncylostomaAncylostomiasisAnimalsAntibodies, HelminthAntigens, HelminthBase SequenceCloning, MolecularCricetinaeHelminth ProteinsImmunizationImmunization, PassiveInjections, SubcutaneousMesocricetusMolecular Sequence DataRecombinant ProteinsSequence Analysis, DNAVaccinesConceptsAdult worm RNANovel immunoreactive proteinsCDNA predictsImmunization of hamstersES productsEnzyme-linked immunosorbentProtein 2Immunoreactive proteinExcretory-secretory productsHookworm anaemiaOral immunizationGlobal morbidityReverse transcription-PCRInfected hamstersInduced antibodiesHigh-pressure liquid chromatographyReverse-phase high-pressure liquid chromatographyNormal serumAdult hookwormsComplete recoveryVaccinationHyperimmune rabbitAnemiaImmunizationAdult wormsMolecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay
Chu D, Bungiro RD, Ibanez M, Harrison LM, Campodonico E, Jones BF, Mieszczanek J, Kuzmic P, Cappello M. Molecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay. Infection And Immunity 2004, 72: 2214-2221. PMID: 15039345, PMCID: PMC375216, DOI: 10.1128/iai.72.4.2214-2221.2004.Peer-Reviewed Original ResearchConceptsGrowth delayAdult hookwormsIron deficiency anemiaPolyclonal immunoglobulin GDeficiency anemiaHookworm infectionReverse transcription-PCRKunitz-type serine protease inhibitorImmunohistochemistry studiesBroad-spectrum inhibitorPartial protectionMajor causeImmunoglobulin GSerine protease inhibitorIntestinal proteasesThird-stage larvaeTranscription-PCRProtease inhibitorsAnemiaHookwormVivo roleMalnutritionInhibitorsInfected hostPancreatic elastaseAnticoagulant peptides from Ancylostoma caninum are immunologically distinct and localize to separate structures within the adult hookworm
Mieszczanek J, Harrison LM, Vlasuk GP, Cappello M. Anticoagulant peptides from Ancylostoma caninum are immunologically distinct and localize to separate structures within the adult hookworm. Molecular And Biochemical Parasitology 2004, 133: 319-323. PMID: 14698444, DOI: 10.1016/j.molbiopara.2003.10.015.Peer-Reviewed Original Research
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stage
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularisAncylostoma ceylanicum excretory/secretory protein 1: purification and molecular cloning of a major secretory protein from adult hookworms
Bungiro RD, Harrison LM, Cappello M. Ancylostoma ceylanicum excretory/secretory protein 1: purification and molecular cloning of a major secretory protein from adult hookworms. Molecular And Biochemical Parasitology 2002, 119: 147-151. PMID: 11755198, DOI: 10.1016/s0166-6851(01)00408-x.Peer-Reviewed Original Research
2001
Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *
Harrison LM, Nerlinger A, Bungiro RD, Córdova JL, Kuzmič P, Cappello M. Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *. Journal Of Biological Chemistry 2001, 277: 6223-6229. PMID: 11741914, DOI: 10.1074/jbc.m109908200.Peer-Reviewed Original ResearchConceptsCoagulation factor XaAdult A. ceylanicumHookworm speciesHuman vaccine developmentIron deficiency anemiaAnticoagulant activityFactor Xa inhibitory activityFactor Xa activityDog hookworm Ancylostoma caninumFactor XaXa inhibitory activityMechanism of actionFirst anticoagulantGastrointestinal hemorrhageDeficiency anemiaReverse transcription-PCRSpecies of hookwormXa activityPeptide-1Vaccine developmentHookworm Ancylostoma caninumA. ceylanicumAncylostoma ceylanicumHookwormAncylostoma caninum
2000
A Broad Spectrum Kunitz Type Serine Protease Inhibitor Secreted by the Hookworm Ancylostoma ceylanicum *
Milstone A, Harrison L, Bungiro R, Kuzmic̆ P, Cappello M. A Broad Spectrum Kunitz Type Serine Protease Inhibitor Secreted by the Hookworm Ancylostoma ceylanicum *. Journal Of Biological Chemistry 2000, 275: 29391-29399. PMID: 10893410, DOI: 10.1074/jbc.m002715200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsBase SequenceHydrolysisMolecular Sequence DataSerine Proteinase InhibitorsConceptsKunitz-type serine protease inhibitorType serine protease inhibitorSerine protease inhibitorSecretory signal sequenceBlood-feeding hookwormsPET-28a plasmidSoluble protein extractsTight binding inhibitorMature proteinCDNA correspondingSignal sequenceParasitic nematodesInfective third-stage larvaeProtease inhibitorsRecombinant proteinsParasite survivalMolecular mechanismsMolecular massProtein extractsEscherichia coliExcretory/secretory productsSerine proteasesThird-stage larvaeAdult A. ceylanicumIron deficiency anemia
1998
Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans
Cappello M, Li S, Chen X, Li C, Harrison L, Narashimhan S, Beard C, Aksoy S. Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14290-14295. PMID: 9826693, PMCID: PMC24366, DOI: 10.1073/pnas.95.24.14290.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnticoagulantsAntithrombin ProteinsAntithrombinsBase SequenceBloodCloning, MolecularDigestive SystemDNA, ComplementaryEatingEscherichia coliFemaleGene Expression RegulationInsect ProteinsMaleMolecular Sequence DataPupaRecombinant ProteinsRNA, MessengerSalivary GlandsThrombinTranscription, GeneticTsetse FliesConceptsTsetse thrombin inhibitorPutative secretory signal peptideNative proteinGlossina morsitans morsitansFull-length cDNADegenerate oligonucleotide probeSecretory signal peptideSalivary gland cDNA libraryLack of homologyMorsitans morsitansUntranslated segmentSignal peptideInhibitor geneCDNA librarySingle copyTsetse genomeAdult tsetse fliesFamily of moleculesSegment codesEscherichia coliOptimal inhibitory activityOligonucleotide probesTsetse fliesSalivary glandsGut tissue
1996
Molecular Approaches to Vaccinating against Hookworm Disease
Hotez P, Hawdon J, Cappello M, Jones B, Ghosh K, Volvovitz F, Shu-Hua X. Molecular Approaches to Vaccinating against Hookworm Disease. Pediatric Research 1996, 40: 515-521. PMID: 8888276, DOI: 10.1203/00006450-199610000-00001.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsLarval antigensVaccine antigensInfective hookworm larvaeProtective immunityHookworm infectionAdjuvant formulationsDrug chemotherapyAlternative vaccinesVaccine routeLive vaccineGenetic immunizationVaccine developmentHookworm larvaeHookworm diseaseParasitic diseasesAntigenPhase IVaccineAncylostoma caninumProtein 1ImmunizationTarget populationAcceptable approachDiseaseASP-2Ancylostoma caninum anticoagulant peptide: cloning by PCR and expression of soluble, active protein in E. coli
Cappello M, Hawdon J, Jones B, Kennedy W, Hotez P. Ancylostoma caninum anticoagulant peptide: cloning by PCR and expression of soluble, active protein in E. coli. Molecular And Biochemical Parasitology 1996, 80: 113-117. PMID: 8885227, DOI: 10.1016/0166-6851(96)02658-8.Peer-Reviewed Original ResearchIsolation and characterization of the tsetse thrombin inhibitor: a potent antithrombotic peptide from the saliva of Glossina morsitans morsitans.
Cappello M, Bergum PW, Vlasuk GP, Furmidge BA, Pritchard DI, Aksoy S. Isolation and characterization of the tsetse thrombin inhibitor: a potent antithrombotic peptide from the saliva of Glossina morsitans morsitans. American Journal Of Tropical Medicine And Hygiene 1996, 54: 475-80. PMID: 8644901, DOI: 10.4269/ajtmh.1996.54.475.Peer-Reviewed Original ResearchAnticoagulant repertoire of the hookworm Ancylostoma caninum.
Stassens P, Bergum P, Gansemans Y, Jespers L, Laroche Y, Huang S, Maki S, Messens J, Lauwereys M, Cappello M, Hotez P, Lasters I, Vlasuk G. Anticoagulant repertoire of the hookworm Ancylostoma caninum. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2149-2154. PMID: 8700900, PMCID: PMC39925, DOI: 10.1073/pnas.93.5.2149.Peer-Reviewed Original ResearchConceptsHookworm Ancylostoma caninumReactive site residuesFVIIa/TFBlood coagulation factor VIIaBlood coagulation factor XaMolecular cloningSerine protease inhibitorMammalian hostsCysteine residuesHost hemostasisSite residuesCoagulation factor VIIaHomologous regionsSequence positionsAncylostoma caninumNematode AscarisCatalytic centerBinary complexUnique mechanismCoagulation factor XaProtease inhibitorsResiduesInhibitorsTissue factorThird form