2009
Ligand-modified gene carriers increased uptake in target cells but reduced DNA release and transfection efficiency
Cu Y, LeMoëllic C, Caplan MJ, Saltzman WM. Ligand-modified gene carriers increased uptake in target cells but reduced DNA release and transfection efficiency. Nanomedicine Nanotechnology Biology And Medicine 2009, 6: 334-343. PMID: 19800989, PMCID: PMC2847641, DOI: 10.1016/j.nano.2009.09.001.Peer-Reviewed Original ResearchConceptsTransfection efficiencyDNA deliveryDNA release rateParticle carriersUnmodified particlesCLINICAL EDITORDrug carriersGene carriersPayload releaseBovine serum albuminCell uptakeParticle surfaceDNA releasePolymer drug carriersPLGASpecific cellsBiodegradable polymersCarriersSerum albuminRelease rateBSAParticlesHigh densityHigh uptakeDelivery
1993
Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly
Gottardi CJ, Caplan MJ. Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly. Journal Of Biological Chemistry 1993, 268: 14342-14347. PMID: 8390991, DOI: 10.1016/s0021-9258(19)85246-8.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunitK-ATPase beta subunitAlpha subunitBeta subunitCell surface expressionTerminal halfK-ATPaseCell surface deliveryEfficient cell surface expressionK-ATPase alphaNH2-terminal halfCOS-1 cellsIon-transporting ATPasesProtein domainsK-ATPase enzymeSubunit assemblySurface deliveryIntracellular vesiclesSubunit chimerasIndividual subunitsActive enzymeMolecular requirementsSubunitsCell surfaceBeta protein
1989
Polarized distribution of Na+,K+-ATPase in giant cells elicited in vivo and in vitro.
Vignery A, Niven-Fairchild T, Ingbar DH, Caplan M. Polarized distribution of Na+,K+-ATPase in giant cells elicited in vivo and in vitro. Journal Of Histochemistry & Cytochemistry 1989, 37: 1265-1271. PMID: 2546991, DOI: 10.1177/37.8.2546991.Peer-Reviewed Original ResearchConceptsPlasma membranePolarized distributionAdherent plasma membraneGiant cell differentiationLysosomal membrane antigenAlpha subunit synthesisSuitable model systemSpecialized functionsCell differentiationBiochemical studiesCell surfaceLysosomal componentsCell formationATPase expressionGiant cell formationModel systemATPaseExpressionCells
1985
Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA.
Schneider JW, Mercer RW, Caplan M, Emanuel JR, Sweadner KJ, Benz EJ, Levenson R. Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 6357-6361. PMID: 2994074, PMCID: PMC391053, DOI: 10.1073/pnas.82.18.6357.Peer-Reviewed Original ResearchConceptsRat brain NaBrain NaK-ATPase alpha subunitRat brainAlpha subunitCDNA clonesK-ATPase antibodiesCell linesFusion proteinCDNA insertK-ATPaseLambda gt11 cDNA expression libraryAlpha-subunit cDNACDNA fusion proteinDifferent rat tissuesK-ATPase polypeptidesTissue-specific patternsPositive phage clonesAmino acid sequenceAntibody reactiveCDNA expression libraryParental HeLa cellsMonoclonal antibodiesDog kidneyHuman cell lines