2022
Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity
Strine M, Alfajaro M, Graziano V, Song J, Hsieh L, Hill R, Guo J, VanDussen K, Orchard R, Baldridge M, Lee S, Wilen C. Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity. Cell Reports 2022, 41: 111593. PMID: 36351394, PMCID: PMC9662704, DOI: 10.1016/j.celrep.2022.111593.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaliciviridae InfectionsHumansMiceMice, Inbred C57BLNorovirusTropismViral Tropism
2021
CD300lf Conditional Knockout Mouse Reveals Strain-Specific Cellular Tropism of Murine Norovirus
Graziano VR, Alfajaro MM, Schmitz CO, Filler RB, Strine MS, Wei J, Hsieh LL, Baldridge MT, Nice TJ, Lee S, Orchard RC, Wilen CB. CD300lf Conditional Knockout Mouse Reveals Strain-Specific Cellular Tropism of Murine Norovirus. Journal Of Virology 2021, 95: 10.1128/jvi.01652-20. PMID: 33177207, PMCID: PMC7925115, DOI: 10.1128/jvi.01652-20.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaliciviridae InfectionsEpithelial CellsFemaleHost-Pathogen InteractionsImmunity, InnateIntestinesMaleMiceMice, KnockoutNorovirusReceptors, ImmunologicViral TropismConceptsConditional knockout miceIntestinal epithelial cellsCell tropismKnockout miceTuft cellsDendritic cellsMyelomonocytic cellsB cellsCellular tropismMurine norovirusEpithelial cellsViral RNA levelsInnate immune responseCause of gastroenteritisMNoV infectionCell typesViral loadGastrointestinal infectionsReceptor expressionImmunocompetent humansImmune responseCell type-specific rolesMouse modelIntestinal tissueMNoV
2018
Phosphatidylinositol 3-Kinase/Akt and MEK/ERK Signaling Pathways Facilitate Sapovirus Trafficking and Late Endosomal Acidification for Viral Uncoating in LLC-PK Cells
Soliman M, Kim D, Park J, Kim J, Alfajaro M, Baek Y, Cho E, Park C, Kang M, Park S, Cho K. Phosphatidylinositol 3-Kinase/Akt and MEK/ERK Signaling Pathways Facilitate Sapovirus Trafficking and Late Endosomal Acidification for Viral Uncoating in LLC-PK Cells. Journal Of Virology 2018, 92: 10.1128/jvi.01674-18. PMID: 30282712, PMCID: PMC6258943, DOI: 10.1128/jvi.01674-18.Peer-Reviewed Original ResearchConceptsMEK/ERKCell surface carbohydrate receptorsLate endosomesPI3K/AktSmall interfering RNAsEndosomal acidificationPI3KEarly endosomesExtracellular signal-regulated kinaseViral uncoatingSignal-regulated kinaseV-ATPase proton pumpCell surface receptorsHost cell entryEarly activationEntry processERK moleculesInterfering RNAsEndosomesCarbohydrate receptorsUse of inhibitorsProton pumpERKSurface receptorsAktPorcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II
Soliman M, Kim D, Kim C, Seo J, Kim J, Park J, Alfajaro M, Baek Y, Cho E, Park S, Kang M, Chang K, Goodfellow I, Cho K. Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II. Veterinary Research 2018, 49: 92. PMID: 30223898, PMCID: PMC6142377, DOI: 10.1186/s13567-018-0584-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaliciviridae InfectionsCholesterolClathrinDynamin IIEndocytosisGastroenteritisHeLa CellsHumansLLC-PK1 CellsSapovirusSwineSwine DiseasesConceptsDynamin IIActin rearrangementLate endosomesDN mutantsLLC-PK cellsDynamin GTPase activityClathrin-mediated endocytosisCowden strainClathrin-mediated internalizationDominant negative mutantCholesterol-dependent endocytosisInhibition of caveolaeVesicle internalizationCholesterol-sequestering drugEndosomal traffickingEarly endosomesSiRNA depletionNegative mutantGTPase activityClathrinEndocytosisEndosomal acidificationMutantsEndosomesCell entryBovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens
Cho E, Soliman M, Alfajaro M, Kim J, Seo J, Park J, Kim D, Baek Y, Kang M, Park S, Le Pendu J, Cho K. Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens. Journal Of Virology 2018, 92: 10.1128/jvi.02160-17. PMID: 29467317, PMCID: PMC5899197, DOI: 10.1128/jvi.02160-17.Peer-Reviewed Original ResearchConceptsFucose epitopesTerminal sialic acidHost speciesVirus-like particlesHisto-blood group antigensCell surface carbohydrate moietiesMammalian host speciesEnteric calicivirusesAttachment factorsSialic acidCultured cell linesBovine digestive tractSurface carbohydrate moietiesCell carbohydratesHost rangeType speciesViral generaHBGA recognitionCarbohydrate moietyBiochemical assaysSpecific enzymesCellular receptorsSynthetic histo-blood group antigensReplication cycleSpecies
2017
Activation of COX-2/PGE2 Promotes Sapovirus Replication via the Inhibition of Nitric Oxide Production
Alfajaro M, Choi J, Kim D, Seo J, Kim J, Park J, Soliman M, Baek Y, Cho E, Kwon J, Kwon H, Park S, Lee W, Kang M, Hosmillo M, Goodfellow I, Cho K. Activation of COX-2/PGE2 Promotes Sapovirus Replication via the Inhibition of Nitric Oxide Production. Journal Of Virology 2017, 91: 10.1128/jvi.01656-16. PMID: 27881647, PMCID: PMC5244346, DOI: 10.1128/jvi.01656-16.Peer-Reviewed Original ResearchConceptsCOX/PGENonsteroidal anti-inflammatory drugsAnti-inflammatory drugsCOX-2Sapovirus infectionAcute gastroenteritisProstaglandin ECOX-1/2 inhibitor indomethacinAntiviral effector mechanismsCOX-2 specific inhibitor NS-398Severe acute gastroenteritisCOX-1 levelsInhibitor NS-398New targetsNitric oxide synthaseProduction of PGENitric oxide productionCOX-2 mRNASmall interfering RNAsPorcine sapovirusMajor etiological agentPotential new targetsInhibitor indomethacinOxide synthaseEffector mechanisms
2014
Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus
Kim D, Hosmillo M, Alfajaro M, Kim J, Park J, Son K, Ryu E, Sorgeloos F, Kwon H, Park S, Lee W, Cho D, Kwon J, Choi J, Kang M, Goodfellow I, Cho K. Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus. PLOS Pathogens 2014, 10: e1004172. PMID: 24901849, PMCID: PMC4047124, DOI: 10.1371/journal.ppat.1004172.Peer-Reviewed Original ResearchConceptsHisto-blood group antigensFunctional receptorsSambucus nigra lectinSialic acidGroup antigensTreatment of cellsSynthetic histo-blood group antigensViral attachmentVirus bindingMaackia amurensis lectinPorcine sapovirusVibrio cholerae neuraminidaseRed blood cellsIntestinal tissue sectionsAcute gastroenteritisImportant causeInfectionBlood cellsVirus attachmentReceptorsPSAVSapovirusCellular receptorsTissue sectionsGlycoprotein acts