2021
Restriction of SARS-CoV-2 replication by targeting programmed −1 ribosomal frameshifting
Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach BD, Guo JU. Restriction of SARS-CoV-2 replication by targeting programmed −1 ribosomal frameshifting. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2023051118. PMID: 34185680, PMCID: PMC8256030, DOI: 10.1073/pnas.2023051118.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 replicationSARS-CoV-2Severe acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Respiratory syndrome coronavirus 2Syndrome coronavirus 2Vero E6 cellsHigh-throughput compound screenOpen reading frame 1bEffective antiviral strategiesCoronavirus 2E6 cellsAntiviral strategiesViral gene expressionCompound screenFluoroquinolone antibacterialsFrame 1bGene expression
2020
Mouse model of SARS-CoV-2 reveals inflammatory role of type I interferon signaling
Israelow B, Song E, Mao T, Lu P, Meir A, Liu F, Alfajaro MM, Wei J, Dong H, Homer RJ, Ring A, Wilen CB, Iwasaki A. Mouse model of SARS-CoV-2 reveals inflammatory role of type I interferon signaling. Journal Of Experimental Medicine 2020, 217: e20201241. PMID: 32750141, PMCID: PMC7401025, DOI: 10.1084/jem.20201241.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin-Converting Enzyme 2AnimalsBetacoronavirusCell Line, TumorCoronavirus InfectionsCOVID-19DependovirusDisease Models, AnimalFemaleHumansInflammationInterferon Type ILungMaleMiceMice, Inbred C57BLMice, TransgenicPandemicsParvoviridae InfectionsPeptidyl-Dipeptidase APneumonia, ViralSARS-CoV-2Signal TransductionVirus ReplicationConceptsSARS-CoV-2Type I interferonMouse modelI interferonRobust SARS-CoV-2 infectionSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2SARS-CoV-2 infectionRespiratory syndrome coronavirus 2SARS-CoV-2 replicationCOVID-19 patientsSyndrome coronavirus 2Patient-derived virusesSignificant fatality ratePathological findingsInflammatory rolePathological responseEnzyme 2Receptor angiotensinFatality rateVaccine developmentGenetic backgroundViral replicationCoronavirus diseaseMice
2018
Feline calicivirus- and murine norovirus-induced COX-2/PGE2 signaling pathway has proviral effects
Alfajaro M, Cho E, Park J, Kim J, Soliman M, Baek Y, Kang M, Park S, Cho K. Feline calicivirus- and murine norovirus-induced COX-2/PGE2 signaling pathway has proviral effects. PLOS ONE 2018, 13: e0200726. PMID: 30021004, PMCID: PMC6051663, DOI: 10.1371/journal.pone.0200726.Peer-Reviewed Original ResearchConceptsCOX-2/COX-1/Production of PGE2COX-2 enzymePharmacological inhibitorsProviral effectCOX-1COX-2/PGE2Potential therapeutic candidateAddition of PGE2Small interfering RNAsSame virus familyReplication of virusesInfection of cellsTime-dependent mannerAntiviral effectMNV infectionTherapeutic candidatePGE2Virus replicationMNV replicationPathophysiological conditionsInhibitory effectGenus SapovirusInfection
2017
Activation of COX-2/PGE2 Promotes Sapovirus Replication via the Inhibition of Nitric Oxide Production
Alfajaro M, Choi J, Kim D, Seo J, Kim J, Park J, Soliman M, Baek Y, Cho E, Kwon J, Kwon H, Park S, Lee W, Kang M, Hosmillo M, Goodfellow I, Cho K. Activation of COX-2/PGE2 Promotes Sapovirus Replication via the Inhibition of Nitric Oxide Production. Journal Of Virology 2017, 91: 10.1128/jvi.01656-16. PMID: 27881647, PMCID: PMC5244346, DOI: 10.1128/jvi.01656-16.Peer-Reviewed Original ResearchConceptsCOX/PGENonsteroidal anti-inflammatory drugsAnti-inflammatory drugsCOX-2Sapovirus infectionAcute gastroenteritisProstaglandin ECOX-1/2 inhibitor indomethacinAntiviral effector mechanismsCOX-2 specific inhibitor NS-398Severe acute gastroenteritisCOX-1 levelsInhibitor NS-398New targetsNitric oxide synthaseProduction of PGENitric oxide productionCOX-2 mRNASmall interfering RNAsPorcine sapovirusMajor etiological agentPotential new targetsInhibitor indomethacinOxide synthaseEffector mechanisms