2023
Adaptation of Nontypeable Haemophilus influenzae in Human Airways in COPD: Genome Rearrangements and Modulation of Expression of HMW1 and HMW2
Murphy T, Kirkham C, D’Mello A, Sethi S, Pettigrew M, Tettelin H. Adaptation of Nontypeable Haemophilus influenzae in Human Airways in COPD: Genome Rearrangements and Modulation of Expression of HMW1 and HMW2. MBio 2023, 14: e00140-23. PMID: 36927061, PMCID: PMC10127715, DOI: 10.1128/mbio.00140-23.Peer-Reviewed Original ResearchMeSH KeywordsAdhesins, BacterialAdultHaemophilus InfectionsHaemophilus influenzaeHumansInflammationPulmonary Disease, Chronic ObstructiveRespiratory SystemConceptsChronic obstructive pulmonary diseaseNontypeable Haemophilus influenzaeObstructive pulmonary diseaseRespiratory epithelial cellsHuman airwaysHaemophilus influenzaeAirway inflammationLung functionMechanisms of persistencePulmonary diseaseEpithelial cellsNovel interventionsChronic lower airway infectionsProgressive lossAirways of adultsLower airway infectionHuman respiratory epithelial cellsCommon debilitating disorderStrains of NTHiNTHi infectionAirway infectionLower airwaysCommon causeSequential isolatesSerial isolates
2019
Non-typeable Haemophilus influenzae isolates from patients with chronic obstructive pulmonary disease contain new phase-variable modA methyltransferase alleles controlling phasevarions
Atack JM, Murphy TF, Pettigrew MM, Seib KL, Jennings MP. Non-typeable Haemophilus influenzae isolates from patients with chronic obstructive pulmonary disease contain new phase-variable modA methyltransferase alleles controlling phasevarions. Scientific Reports 2019, 9: 15963. PMID: 31685916, PMCID: PMC6828955, DOI: 10.1038/s41598-019-52429-6.Peer-Reviewed Original ResearchConceptsNon-typeable Haemophilus influenzaeChronic obstructive pulmonary diseaseObstructive pulmonary diseaseMiddle ear infectionPulmonary diseaseEar infectionsNTHi strainsHaemophilus influenzaeExacerbation of COPDCOPDMiddle earPatientsAllele distributionInfectionInfluenzaeBacterial pathogensDiseaseMultiple allelic variantsAllelic variantsGene expressionAllelesEpigenetic mechanismsLarge panelExacerbationExpressionDiscovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion
Ahearn C, Kirkham C, Chaves L, Kong Y, Pettigrew M, Murphy T. Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion. Infection And Immunity 2019, 87: 10.1128/iai.00462-19. PMID: 31427451, PMCID: PMC6803334, DOI: 10.1128/iai.00462-19.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseRespiratory epithelial cellsEnzyme-linked immunosorbent assayEpithelial cellsAirways of adultsObstructive pulmonary diseaseCell invasionHuman respiratory epithelial cellsNontypeable Haemophilus influenzaeCell enzyme-linked immunosorbent assayAlveolar epithelial cellsAcute exacerbationLower airwaysPulmonary diseaseSerum IgGNTHi strainsTherapeutic interventionsHaemophilus influenzaeHuman bronchialSurface-exposed epitopesEpithelial cell invasionIsogenic knockout mutantsNTHiImmunosorbent assayIntracellular survival
2018
Changes in IgA Protease Expression Are Conferred by Changes in Genomes during Persistent Infection by Nontypeable Haemophilus influenzae in Chronic Obstructive Pulmonary Disease
Gallo MC, Kirkham C, Eng S, Bebawee RS, Kong Y, Pettigrew MM, Tettelin H, Murphy TF. Changes in IgA Protease Expression Are Conferred by Changes in Genomes during Persistent Infection by Nontypeable Haemophilus influenzae in Chronic Obstructive Pulmonary Disease. Infection And Immunity 2018, 86: 10.1128/iai.00313-18. PMID: 29760213, PMCID: PMC6056860, DOI: 10.1128/iai.00313-18.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseasePersistent infectionPulmonary diseaseIgA proteaseProtease expressionNontypeable Haemophilus influenzaeAcute exacerbationLower airwaysRespiratory tractNTHi strainsHaemophilus influenzaeInfectionNTHiNatural infectionHuman pathobiontAltered expressionPatientsDiseasePersistent strainsExpressionCritical roleExacerbationAirwayInitial acquisitionHaemophilus influenzae genome evolution during persistence in the human airways in chronic obstructive pulmonary disease
Pettigrew MM, Ahearn CP, Gent JF, Kong Y, Gallo MC, Munro JB, D’Mello A, Sethi S, Tettelin H, Murphy TF. Haemophilus influenzae genome evolution during persistence in the human airways in chronic obstructive pulmonary disease. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: e3256-e3265. PMID: 29555745, PMCID: PMC5889651, DOI: 10.1073/pnas.1719654115.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseaseCandidate vaccine antigensHuman airwaysPulmonary diseaseVaccine antigensRespiratory tract pathogensHuman respiratory tractProspective studyTract pathogensNTHi isolatesRespiratory tractAnimal modelsVaccine developmentHuman infectionsAirwayNTHiSurface moleculesAmino acid sequence changesWhole-genome sequencingAntigenBacterial pathogensDiseaseSubset of strainsVirulence functionsAzithromycin Pharmacodynamics against Persistent Haemophilus influenzae in Chronic Obstructive Pulmonary Disease
Tsuji BT, Fisher J, Boadi-Yeboah R, Holden PN, Sethi S, Pettigrew MM, Murphy TF. Azithromycin Pharmacodynamics against Persistent Haemophilus influenzae in Chronic Obstructive Pulmonary Disease. Antimicrobial Agents And Chemotherapy 2018, 62: 10.1128/aac.01995-17. PMID: 29180527, PMCID: PMC5786777, DOI: 10.1128/aac.01995-17.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsAzithromycinHaemophilus InfectionsHaemophilus influenzaeHumansPulmonary Disease, Chronic ObstructiveRespiratory SystemConceptsChronic obstructive pulmonary disease patientsObstructive pulmonary disease patientsChronic obstructive pulmonary diseaseHollow-fiber infection modelStrains of NTHiPulmonary disease patientsObstructive pulmonary diseasePulmonary diseaseConcentration-dependent activityDisease patientsPharmacodynamic profilePharmacodynamic responseSame patientHaemophilus influenzaeInfection modelPatientsAzithromycinPersistent strainsDaysAirwayNTHiPharmacodynamicsInfluenzaeDiseaseHaemophilus
2017
Immunoglobulin A Protease Variants Facilitate Intracellular Survival in Epithelial Cells By Nontypeable Haemophilus influenzae That Persist in the Human Respiratory Tract in Chronic Obstructive Pulmonary Disease
Murphy TF, Kirkham C, Gallo MC, Yang Y, Wilding GE, Pettigrew MM. Immunoglobulin A Protease Variants Facilitate Intracellular Survival in Epithelial Cells By Nontypeable Haemophilus influenzae That Persist in the Human Respiratory Tract in Chronic Obstructive Pulmonary Disease. The Journal Of Infectious Diseases 2017, 216: 1295-1303. PMID: 28968876, PMCID: PMC5853902, DOI: 10.1093/infdis/jix471.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseNontypeable Haemophilus influenzaeObstructive pulmonary diseaseExpression of IgARespiratory epithelial cellsLysosomal-associated membrane protein 1Pulmonary diseaseEpithelial cellsIntracellular survivalHaemophilus influenzaeHuman respiratory epithelial cellsPathogenesis of infectionHuman respiratory tractIgA proteasePassage of strainsMembrane protein 1Host cellsProtease expressionNTHi infectionNTHi isolatesRespiratory tractHost epithelial cellsIgAIntracellular persistenceReduced survivalPanel 6: Vaccines
Pettigrew MM, Alderson MR, Bakaletz LO, Barenkamp SJ, Hakansson AP, Mason KM, Nokso‐Koivisto J, Patel J, Pelton SI, Murphy TF. Panel 6: Vaccines. Otolaryngology 2017, 156: s76-s87. PMID: 28372533, PMCID: PMC5505493, DOI: 10.1177/0194599816632178.Peer-Reviewed Original ResearchConceptsPneumococcal otitis mediaOtitis mediaVaccine antigensVaccine developmentH influenzaePneumococcal serotypesHaemophilus influenzae protein DSignificant health care burdenAdditional pneumococcal serotypesCorrelates of protectionHealth care burdenRecurrent otitis mediaEffectiveness of vaccinesTympanostomy tube insertionProtein-based vaccinesCandidate vaccine antigensOM pathogensOM visitsPCV effectivenessPneumococcal vaccineRespiratory virusesVaccine protectionTube insertionMoraxella catarrhalisCare burden
2016
Antimicrobial activity of antisense peptide–peptide nucleic acid conjugates against non-typeable Haemophilus influenzae in planktonic and biofilm forms
Otsuka T, Brauer AL, Kirkham C, Sully EK, Pettigrew MM, Kong Y, Geller BL, Murphy TF. Antimicrobial activity of antisense peptide–peptide nucleic acid conjugates against non-typeable Haemophilus influenzae in planktonic and biofilm forms. Journal Of Antimicrobial Chemotherapy 2016, 72: 137-144. PMID: 27986898, PMCID: PMC5161047, DOI: 10.1093/jac/dkw384.Peer-Reviewed Original ResearchDensity, Serotype Diversity, and Fitness of Streptococcus pneumoniae in Upper Respiratory Tract Cocolonization With Nontypeable Haemophilus influenzae
Lewnard JA, Huppert A, Givon-Lavi N, Pettigrew MM, Regev-Yochay G, Dagan R, Weinberger DM. Density, Serotype Diversity, and Fitness of Streptococcus pneumoniae in Upper Respiratory Tract Cocolonization With Nontypeable Haemophilus influenzae. The Journal Of Infectious Diseases 2016, 214: 1411-1420. PMID: 27540112, PMCID: PMC5079371, DOI: 10.1093/infdis/jiw381.Peer-Reviewed Original ResearchConceptsNontypeable Haemophilus influenzaeNTHi colonizationPneumococcal serotypesChildren 2Haemophilus influenzaeStreptococcus pneumoniaeUpper respiratory tract carriageSerotype diversityComplex otitis mediaNasopharyngeal pneumococcal carriagePneumococcal conjugate vaccineImmune-evasive phenotypePneumococcal serotype diversityNTHi carriageConjugate vaccinePneumococcal carriageOtitis mediaStudy visitNeutrophil resistanceCapsular thicknessLogistic regressionDensity associationsCarriageSerotypesColonization densityEffect of Fluoroquinolones and Macrolides on Eradication and Resistance of Haemophilus influenzae in Chronic Obstructive Pulmonary Disease
Pettigrew MM, Tsuji BT, Gent JF, Kong Y, Holden PN, Sethi S, Murphy TF. Effect of Fluoroquinolones and Macrolides on Eradication and Resistance of Haemophilus influenzae in Chronic Obstructive Pulmonary Disease. Antimicrobial Agents And Chemotherapy 2016, 60: 4151-4158. PMID: 27139476, PMCID: PMC4914697, DOI: 10.1128/aac.00301-16.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseaseH. influenzae isolatesH. influenzaePulmonary diseaseFluoroquinolone administrationInfluenzae isolatesDevelopment of resistanceHaemophilus influenzaeEradication of carriageEffects of macrolidesQuinolone resistance-determining regionsMICs of antibioticsEffects of fluoroquinolonesResistance-determining regionMacrolide administrationCOPD patientsEffects of antibioticsAzithromycin MICsAntibiotic useSusceptibility breakpointsAntibiotic susceptibilityMacrolide susceptibilityInfluenzaeAdministration
2015
Epidemiological Markers for Interactions Among Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in Upper Respiratory Tract Carriage
Lewnard JA, Givon-Lavi N, Huppert A, Pettigrew MM, Regev-Yochay G, Dagan R, Weinberger DM. Epidemiological Markers for Interactions Among Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in Upper Respiratory Tract Carriage. The Journal Of Infectious Diseases 2015, 213: 1596-1605. PMID: 26704617, PMCID: PMC4837906, DOI: 10.1093/infdis/jiv761.Peer-Reviewed Original ResearchMeSH KeywordsCarrier StateChild, PreschoolFemaleHaemophilus InfectionsHaemophilus influenzaeHeptavalent Pneumococcal Conjugate VaccineHumansInfantIsraelLongitudinal StudiesMalePneumococcal InfectionsRespiratory Tract InfectionsRisk FactorsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeVaccines, ConjugateConceptsPneumococcal serotype replacementH. influenzaeSerotype replacementS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaePneumococcal conjugate vaccine trialUpper respiratory tract carriagePneumococcal vaccine introductionS. aureus carriageMonths of ageStaphylococcus aureusPCV7 recipientsAureus carriageVaccine introductionVaccine trialsCarriage patternsConfounding factorsClinical interventionsInfluenzaeEpidemiological markersBedouin childrenPneumoniaeCarriageS. aureusExpression of IgA Proteases by Haemophilus influenzae in the Respiratory Tract of Adults With Chronic Obstructive Pulmonary Disease
Murphy TF, Kirkham C, Jones MM, Sethi S, Kong Y, Pettigrew MM. Expression of IgA Proteases by Haemophilus influenzae in the Respiratory Tract of Adults With Chronic Obstructive Pulmonary Disease. The Journal Of Infectious Diseases 2015, 212: 1798-1805. PMID: 25995193, PMCID: PMC4633762, DOI: 10.1093/infdis/jiv299.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseaseH. influenzaeIgA protease genePulmonary diseaseHuman airwaysHaemophilus influenzaeIgA proteaseH. influenzae infectionsHuman respiratory epithelial cellsHuman IgA1Respiratory epithelial cellsInfluenzae infectionRespiratory tractIgA protease activitySputum samplesClinical settingInfluenzaeEpithelial cellsInfectionAirwayIgA1 fragmentsDiseaseIgA1Protease gene
2013
Panel 6: Vaccines
Pelton SI, Pettigrew MM, Barenkamp SJ, Godfroid F, Grijalva CG, Leach A, Patel J, Murphy TF, Selak S, Bakaletz LO. Panel 6: Vaccines. Otolaryngology 2013, 148: e90-e101. PMID: 23536534, PMCID: PMC4029613, DOI: 10.1177/0194599812466535.Peer-Reviewed Original ResearchConceptsAcute otitis mediaPneumococcal conjugate vaccineOtitis mediaDevelopment of correlatesCandidate antigensAnimal modelsClinical efficacy trialsEffectiveness of vaccinesBurden of diseaseNontypeable Haemophilus influenzaeHuman clinical trialsOtitis pathogensConjugate vaccineVaccine strategiesPneumococcal serotypesClinical trialsMoraxella catarrhalisAdditional serotypesNew adjuvantsEfficacy trialsObservational studyFrequent recurrenceChild healthVaccine antigensMore antigens
2012
Bacterial and viral interactions within the nasopharynx contribute to the risk of acute otitis media
Ruohola A, Pettigrew MM, Lindholm L, Jalava J, Räisänen KS, Vainionpää R, Waris M, Tähtinen PA, Laine MK, Lahti E, Ruuskanen O, Huovinen P. Bacterial and viral interactions within the nasopharynx contribute to the risk of acute otitis media. Journal Of Infection 2012, 66: 247-254. PMID: 23266462, PMCID: PMC3571106, DOI: 10.1016/j.jinf.2012.12.002.Peer-Reviewed Original ResearchConceptsRisk of AOMAcute otitis mediaMiddle ear effusionAOM groupRespiratory virusesAOM pathogensAcute symptomsOtitis mediaEar effusionM. catarrhalisAcute inflammatory signsNasopharyngeal bacterial colonizationRespiratory tract infectionsOtoscopic signsTract infectionsInflammatory signsNasopharyngeal samplesSymptomatic childrenNasopharyngeal bacteriaH. influenzaePneumatic otoscopyS. pneumoniaeViral interactionsBacterial colonizationEffusion
2011
Viral-Bacterial Interactions and Risk of Acute Otitis Media Complicating Upper Respiratory Tract Infection
Pettigrew MM, Gent JF, Pyles RB, Miller AL, Nokso-Koivisto J, Chonmaitree T. Viral-Bacterial Interactions and Risk of Acute Otitis Media Complicating Upper Respiratory Tract Infection. Journal Of Clinical Microbiology 2011, 49: 3750-3755. PMID: 21900518, PMCID: PMC3209086, DOI: 10.1128/jcm.01186-11.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesComorbidityHaemophilus influenzaeHuman bocavirusHumansInfantMetapneumovirusMicrobial InteractionsMoraxella catarrhalisOtitis MediaPolymerase Chain ReactionProspective StudiesRespiratory Syncytial VirusesRespiratory Tract InfectionsRisk AssessmentStreptococcus pneumoniaeConceptsUpper respiratory tract infectionAcute otitis mediaRespiratory tract infectionsRespiratory syncytial virusOtitis mediaOtitis media riskTract infectionsViral loadSyncytial virusHaemophilus influenzaeStreptococcus pneumoniaeRespiratory Syncytial Virus LoadMedium-risk factorsRepeated-measures logistic regressionRSV viral loadViral-bacterial interactionsHigh viral loadSpecific virusesBacterial pathogensChildren ages 6Common complicationRespiratory virusesMedium riskProspective studyHuman bocavirus
2009
Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens
Thomas JC, Pettigrew MM. Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens. Methods In Molecular Biology 2009, 493: 179-190. PMID: 18839348, PMCID: PMC3581352, DOI: 10.1007/978-1-59745-523-7_11.Peer-Reviewed Original ResearchConceptsOtitis mediaMolecular epidemiologyMultilocus sequence typingMoraxella catarrhalisHaemophilus influenzaeStreptococcus pneumoniaeSequence typingPulsed-Field Gel ElectrophoresisEpidemiologyBacterial pathogensField gel electrophoresisType of studyResearch settingsCharacterization of isolatesPathogensCatarrhalisInfluenzaePneumoniae
2008
Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC
Pettigrew MM, Gent JF, Revai K, Patel JA, Chonmaitree T. Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2008, 14: 1584-1591. PMID: 18826823, PMCID: PMC2609881, DOI: 10.3201/eid1410.080119.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesFemaleHaemophilus InfectionsHaemophilus influenzaeHumansInfantMaleMoraxella catarrhalisMoraxellaceae InfectionsNasopharynxOtitis MediaProspective StudiesRespiratory Tract InfectionsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeSuperinfectionConceptsUpper respiratory tract infectionH. influenzaeInfectious Diseases journal - CDCRespiratory tract infectionsS. pneumoniae colonizationHealthy children 6Months of ageS. aureusProspective cohortTract infectionsPneumoniae colonizationVaccination strategiesAntimicrobial therapyMoraxella catarrhalisChildren 6M. catarrhalisS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaeBacterial infectionsSpecific bacterial speciesInfluenzaeLikelihood of colonizationCatarrhalisStaphylococcus aureus
2007
Genetic Diversity of Paired Middle-Ear and Pharyngeal Nontypeable Haemophilus influenzae Isolates from Children with Acute Otitis Media
Berrens ZJ, Marrs CF, Pettigrew MM, Sandstedt SA, Patel M, Gilsdorf JR. Genetic Diversity of Paired Middle-Ear and Pharyngeal Nontypeable Haemophilus influenzae Isolates from Children with Acute Otitis Media. Journal Of Clinical Microbiology 2007, 45: 3764-3767. PMID: 17804648, PMCID: PMC2168485, DOI: 10.1128/jcm.00964-07.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseChildEar, MiddleElectrophoresis, Gel, Pulsed-FieldGenetic VariationHaemophilus influenzaeHumansOtitis MediaPharynx
2006
Predictors for Haemophilus influenzae Colonization, Antibiotic Resistance and for Sharing an Identical Isolate Among Children Attending 16 Licensed Day-Care Centers in Michigan
Barbosa-Cesnik C, Farjo RS, Patel M, Gilsdorf J, McCoy SI, Pettigrew MM, Marrs C, Foxman B. Predictors for Haemophilus influenzae Colonization, Antibiotic Resistance and for Sharing an Identical Isolate Among Children Attending 16 Licensed Day-Care Centers in Michigan. The Pediatric Infectious Disease Journal 2006, 25: 219-223. PMID: 16511383, DOI: 10.1097/01.inf.0000202130.78540.28.Peer-Reviewed Original ResearchConceptsDay care centersH. influenzae colonizationNontypable H. influenzaeH. influenzaeOtitis mediaColonized childrenAntibiotic resistanceLicensed day care centersSame day care centerHaemophilus influenzae colonizationModifiable risk factorsRisk factor questionnaireH. influenzae isolatesNontypable Haemophilus influenzaeDay care center directorsIdentical isolatesInfluenzae isolatesPacifier useThroat swabsHygiene habitsRisk factorsTobacco smokeImportant causeHaemophilus influenzaeOverall colonization rate