2021
Computed tomography imaging of macrophage phagocytic activity in abdominal aortic aneurysm
Toczek J, Boodagh P, Sanzida N, Ghim M, Salarian M, Gona K, Kukreja G, Rajendran S, Wei L, Han J, Zhang J, Jung JJ, Graham M, Liu X, Sadeghi MM. Computed tomography imaging of macrophage phagocytic activity in abdominal aortic aneurysm. Theranostics 2021, 11: 5876-5888. PMID: 33897887, PMCID: PMC8058712, DOI: 10.7150/thno.55106.Peer-Reviewed Original ResearchConceptsAbdominal aortic aneurysmExiTron nano 12000AAA outcomePhagocytic activityII infusionAng IIAortic aneurysmAortic wall enhancementAng II infusionCT enhancementAngiotensin II infusionRole of inflammationFeasibility of CTMacrophage phagocytic activityNon-invasive toolAAA inductionCD68 expressionModulatory interventionsMacrophage cell lineInflammatory signalsPatient managementVascular pathologyOutcome studiesAdventitial macrophagesComputed tomography
2020
Accuracy of arterial [18F]-Fluorodeoxyglucose uptake quantification: A kinetic modeling study
Toczek J, Wu J, Hillmer AT, Han J, Esterlis I, Cosgrove KP, Liu C, Sadeghi MM. Accuracy of arterial [18F]-Fluorodeoxyglucose uptake quantification: A kinetic modeling study. Journal Of Nuclear Cardiology 2020, 27: 1578-1581. PMID: 32043239, PMCID: PMC7415600, DOI: 10.1007/s12350-020-02055-x.Peer-Reviewed Original ResearchConceptsMean SUVmaxMean TBRmaxFDG signalAortic wallMaximal standardized uptake valueD-glucose (FDG) PETVessel wall inflammationStandardized uptake valueActivity ex vivoPET imaging agentVascular inflammationWall inflammationRecent guidelinesBlood ratioCardiovascular diseasePrior historyPlasma activityTracer uptakeUptake valueSUVmaxTBRmaxEx vivoArterial wallVascular imagingFDG
2019
Novel Matrix Metalloproteinase 12 Selective Radiotracers for Vascular Molecular Imaging
Toczek J, Bordenave T, Gona K, Kim HY, Beau F, Georgiadis D, Correia I, Ye Y, Razavian M, Jung J, Lequin O, Dive V, Sadeghi MM, Devel L. Novel Matrix Metalloproteinase 12 Selective Radiotracers for Vascular Molecular Imaging. Journal Of Medicinal Chemistry 2019, 62: 9743-9752. PMID: 31603669, PMCID: PMC7054845, DOI: 10.1021/acs.jmedchem.9b01186.Peer-Reviewed Original ResearchConceptsAbdominal aortic aneurysmVascular molecular imagingMurine abdominal aortic aneurysmsMMP-12 gene expressionMatrix metalloproteinase-12Aortic aneurysmInflammatory diseasesAdjacent aortaH postinjectionMMP-12Blood clearanceMetalloproteinase-12Selective radiotracerFurther evaluationMMP-12 inhibitorsMouse bloodHigh uptakeAortaDiseaseMolecular imagingHuman metalloproteasesRadiotracer
2017
Characterization of Macrophage Polarization States Using Combined Measurement of 2-Deoxyglucose and Glutamine Accumulation
Tavakoli S, Downs K, Short JD, Nguyen HN, Lai Y, Jerabek PA, Goins B, Toczek J, Sadeghi MM, Asmis R. Characterization of Macrophage Polarization States Using Combined Measurement of 2-Deoxyglucose and Glutamine Accumulation. Arteriosclerosis Thrombosis And Vascular Biology 2017, 37: 1840-1848. PMID: 28798141, PMCID: PMC8036006, DOI: 10.1161/atvbaha.117.308848.Peer-Reviewed Original ResearchConceptsMacrophage activation statesGlutamine accumulationVulnerable plaquesAtherosclerotic lesionsLow-density lipoprotein receptor-null miceF-fluorodeoxyglucose positron emission tomographyReceptor null miceF-fluorodeoxyglucose uptakeHigh-fat dietVessel wall inflammationMacrophage polarization stateEarly promising resultsActivation statePositron emission tomographyMurine peritoneal macrophagesProinflammatory stateInflammatory stateWall inflammationNecrosis factorF-fluorodeoxyglucosePolarization phenotypesInterleukin-4
2016
Rac2 Modulates Atherosclerotic Calcification by Regulating Macrophage Interleukin-1&bgr; Production
Ceneri N, Zhao L, Young BD, Healy A, Coskun S, Vasavada H, Yarovinsky TO, Ike K, Pardi R, Qin L, Qin L, Tellides G, Hirschi K, Meadows J, Soufer R, Chun HJ, Sadeghi M, Bender JR, Morrison AR. Rac2 Modulates Atherosclerotic Calcification by Regulating Macrophage Interleukin-1&bgr; Production. Arteriosclerosis Thrombosis And Vascular Biology 2016, 37: 328-340. PMID: 27834690, PMCID: PMC5269510, DOI: 10.1161/atvbaha.116.308507.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAortic DiseasesApolipoproteins EAtherosclerosisCells, CulturedCoronary Artery DiseaseCoronary VesselsFemaleGenetic Predisposition to DiseaseHumansInflammation MediatorsInterleukin 1 Receptor Antagonist ProteinInterleukin-1betaMacrophagesMaleMice, Inbred C57BLMice, KnockoutMuscle, Smooth, VascularMyocytes, Smooth MuscleNeuropeptidesPhenotypePlaque, AtheroscleroticPrognosisRac GTP-Binding ProteinsRac1 GTP-Binding ProteinSignal TransductionTransfectionUp-RegulationVascular CalcificationConceptsCoronary calcium burdenIL-1β expressionCalcium burdenSerum IL-1β levelsElevated IL-1βIL-1β levelsCoronary artery diseaseInterleukin-1β expressionCalcified coronary arteryCardiovascular deathCardiovascular eventsArtery diseaseIndependent predictorsClinical outcomesVascular calcificationCoronary arteryIL-1βPlaque calciumAtherosclerotic calcificationExperimental atherogenesisInflammatory regulatorsMacrophage interleukinAtherosclerotic plaquesTherapeutic targetProgressive calcification
2011
Atherosclerosis Plaque Heterogeneity and Response to Therapy Detected by In Vivo Molecular Imaging of Matrix Metalloproteinase Activation
Razavian M, Tavakoli S, Zhang J, Nie L, Dobrucki LW, Sinusas AJ, Azure M, Robinson S, Sadeghi MM. Atherosclerosis Plaque Heterogeneity and Response to Therapy Detected by In Vivo Molecular Imaging of Matrix Metalloproteinase Activation. Journal Of Nuclear Medicine 2011, 52: 1795-1802. PMID: 21969358, PMCID: PMC3235922, DOI: 10.2967/jnumed.111.092379.Peer-Reviewed Original ResearchConceptsHigh-fat dietSPECT/CTTracer uptakeMMP activationNormal chowMatrix metalloproteinasesApolipoprotein-deficient miceOil Red O stainingDevelopment of atherosclerosisMatrix metalloproteinase activationRed O stainingAtherosclerotic mouse aortasOil Red OHFD withdrawalMolecular imagingAreas of discordanceDietary modificationMacrophage infiltrationPlaque presenceMacrophage contentPlaque areaPlaque biologyAtherosclerotic plaquesMMP expressionMetalloproteinase activation
2005
αvβ3‐Targeted detection of arteriopathy in transplanted human coronary arteries: an autoradiographic study
Zhang J, Krassilnikova S, Gharaei AA, Fassaei HR, Esmailzadeh L, Asadi A, Edwards DS, Harris TD, Azure M, Tellides G, Sinusas AJ, Zaret BL, Bender JR, Sadeghi MM. αvβ3‐Targeted detection of arteriopathy in transplanted human coronary arteries: an autoradiographic study. The FASEB Journal 2005, 19: 1857-1859. PMID: 16150802, DOI: 10.1096/fj.05-4130fje.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAutoradiographyCarotid Artery DiseasesCell MovementCell ProliferationCell TransplantationCells, CulturedChimeraCoronary VesselsDensitometryEndothelium, VascularHeart TransplantationHeterocyclic Compounds, 1-RingHumansImmunohistochemistryIntegrin alphaVbeta3Ki-67 AntigenLeukocytes, MononuclearMiceMice, SCIDMicroscopy, FluorescenceOrganometallic CompoundsRecombinant Fusion ProteinsTime FactorsTissue TransplantationUp-RegulationVascular DiseasesConceptsPeripheral blood mononuclear cellsGraft arteriopathyHuman coronary arteriesCoronary arteryAllogeneic human peripheral blood mononuclear cellsHuman/mouse chimeric modelAlphavbeta3 expressionHuman peripheral blood mononuclear cellsProliferative processesSevere combined immunodeficiency miceLate graft failureBlood mononuclear cellsCombined immunodeficiency miceAlphavbeta3 integrinSpecificity of uptakeCardiac transplantationConcentric narrowingGraft failureMononuclear cellsImmunodeficiency miceVascular remodelingNeointima formationNative aortaChimeric modelAutoradiographic study