2017
Characterization of Macrophage Polarization States Using Combined Measurement of 2-Deoxyglucose and Glutamine Accumulation
Tavakoli S, Downs K, Short JD, Nguyen HN, Lai Y, Jerabek PA, Goins B, Toczek J, Sadeghi MM, Asmis R. Characterization of Macrophage Polarization States Using Combined Measurement of 2-Deoxyglucose and Glutamine Accumulation. Arteriosclerosis Thrombosis And Vascular Biology 2017, 37: 1840-1848. PMID: 28798141, PMCID: PMC8036006, DOI: 10.1161/atvbaha.117.308848.Peer-Reviewed Original ResearchConceptsMacrophage activation statesGlutamine accumulationVulnerable plaquesAtherosclerotic lesionsLow-density lipoprotein receptor-null miceF-fluorodeoxyglucose positron emission tomographyReceptor null miceF-fluorodeoxyglucose uptakeHigh-fat dietVessel wall inflammationMacrophage polarization stateEarly promising resultsActivation statePositron emission tomographyMurine peritoneal macrophagesProinflammatory stateInflammatory stateWall inflammationNecrosis factorF-fluorodeoxyglucosePolarization phenotypesInterleukin-4
2016
Differential Regulation of Macrophage Glucose Metabolism by Macrophage Colony-stimulating Factor and Granulocyte-Macrophage Colony-stimulating Factor: Implications for 18F FDG PET Imaging of Vessel Wall Inflammation.
Tavakoli S, Short JD, Downs K, Nguyen HN, Lai Y, Zhang W, Jerabek P, Goins B, Sadeghi MM, Asmis R. Differential Regulation of Macrophage Glucose Metabolism by Macrophage Colony-stimulating Factor and Granulocyte-Macrophage Colony-stimulating Factor: Implications for 18F FDG PET Imaging of Vessel Wall Inflammation. Radiology 2016, 283: 87-97. PMID: 27849433, PMCID: PMC5375627, DOI: 10.1148/radiol.2016160839.Peer-Reviewed Original ResearchConceptsM-CSFMurine atherosclerotic aortasStable isotope tracingActivation stateExtracellular acidification rateGM-CSFMitochondrial biogenesisMacrophage colony-stimulating factorGlycolytic enzymesGlucose fluxMurine peritoneal macrophagesDistinct activation statesGlycolytic pathwayGlucose transporterCritical glycolytic enzymeGlycolytic metabolismAcidification rateIsotope tracingIntermediary metabolitesGlucose uptakeImmunometabolic phenotypeMurine atherosclerotic plaquesCell culturesSimilar levelsOxidative metabolism