2024
42. STRESS EXPOSURE DYNAMICALLY REGULATES EQTL ACTIVITY IN THE POST-MORTEM BRAIN AND IN HIPSC-DERIVED NEURONS
Seah C, Signer R, Young H, Hicks E, Rusielewicz T, Bader H, Xu C, Breen M, Paull D, Yehuda R, Girgenti M, Brennand K, Huckins L. 42. STRESS EXPOSURE DYNAMICALLY REGULATES EQTL ACTIVITY IN THE POST-MORTEM BRAIN AND IN HIPSC-DERIVED NEURONS. European Neuropsychopharmacology 2024, 87: 71-72. DOI: 10.1016/j.euroneuro.2024.08.156.Peer-Reviewed Original ResearchPost-mortem brainsTranscription factor binding sitesAbsence of cellular stressCombat-exposed veteransFactor binding sitesImpact gene expressionBinding sitesGR binding sitesPositive regulatory activityMotif enrichmentSequence readsCRISPRi screenOpen chromatinFunctional annotationBrain regionsTraumatic stressCRISPR screensEQTLTraumatic experiencesLeading locusPTSDPerturbed genesRegulatory architectureTranscriptomic activityTranscriptomic response
2021
Gene expression in the dorsolateral and ventromedial prefrontal cortices implicates immune-related gene networks in PTSD
Logue MW, Zhou Z, Morrison FG, Wolf EJ, Daskalakis NP, Chatzinakos C, Georgiadis F, Labadorf AT, Girgenti MJ, Young KA, Williamson DE, Zhao X, Grenier JG, Group T, Huber BR, Miller MW. Gene expression in the dorsolateral and ventromedial prefrontal cortices implicates immune-related gene networks in PTSD. Neurobiology Of Stress 2021, 15: 100398. PMID: 34646915, PMCID: PMC8498459, DOI: 10.1016/j.ynstr.2021.100398.Peer-Reviewed Original ResearchPrefrontal cortexVentromedial prefrontal cortexDepression casesHistory of PTSDDepression-associated genesImmune-related networkBrain BankImmune regulationGene expressionIndependent cohortBrain regionsEvidence of replicationPTSD casesMultiple testing correctionImmune genesPTSDCo-expression network analysisCortexEffect sizeUpstream regulatorRNA sequencingRecent studiesGenetic modelsExpressionCandidate gene analysis
2020
Stress and Its Impact on the Transcriptome
Girgenti MJ, Pothula S, Newton SS. Stress and Its Impact on the Transcriptome. Biological Psychiatry 2020, 90: 102-108. PMID: 33637305, PMCID: PMC8213869, DOI: 10.1016/j.biopsych.2020.12.011.Peer-Reviewed Original ResearchConceptsTranscriptomic dysregulationMajor gene expression changesGene expression changesDevelopmental time pointsAnimal modelsGenomic studiesNeuropsychiatric disordersTranscriptomic methodsAnimal kingdomComparable animal modelsGene expressionExpression changesEarly life stressPhenotypic signaturesSex-specific changesTransgenerational impactTranscriptomeSingle cellsEntire brain regionsPowerful approachDiseased brainBrain regionsChronic stressMolecular profileSame disorder
2018
Molecular and cellular studies of PTSD: Postmortem transcriptome analysis and novel therapeutic targets
Duman RS, Girgenti MJ. Molecular and cellular studies of PTSD: Postmortem transcriptome analysis and novel therapeutic targets. Journal Of Neuroscience Research 2018, 97: 292-299. PMID: 30136735, DOI: 10.1002/jnr.24306.Peer-Reviewed Original ResearchConceptsFear memoryPrefrontal cortexPTSD patientsFear memory processesNeurobiology of PTSDMemory processesInhibitory controlNeurobiological underpinningsPFC subregionsPTSD subjectsPTSDMemory studiesBrain regionsMemorySpecific gene expression alterationsNovel therapeutic targetPostmortem brain tissueEfficacious treatmentNeurobiologySynaptic alterationsSynapse numberEarlier findingsRodent modelsTherapeutic targetGene expression alterations
2017
Molecular and Cellular Effects of Traumatic Stress: Implications for PTSD
Girgenti MJ, Hare BD, Ghosal S, Duman RS. Molecular and Cellular Effects of Traumatic Stress: Implications for PTSD. Current Psychiatry Reports 2017, 19: 85. PMID: 28944401, PMCID: PMC5907804, DOI: 10.1007/s11920-017-0841-3.Peer-Reviewed Original ResearchConceptsPathophysiology of PTSDPreclinical modelsReviewPosttraumatic stress disorderPeripheral blood studiesMolecular changesGlucocorticoid receptor signalingPrimary brain regionsPeripheral bloodInflammatory systemBlood studiesGlucocorticoid signalingHuman studiesPTSD patientsMolecular alterationsBrain regionsStress disorderAltered gene expressionPathophysiologyReceptor signalingCellular pathophysiologyNeurobiological determinantsCellular effectsMechanistic evidencePTSDPatients