2024
Intravenous infusion of auto-serum-expanded autologous mesenchymal stem cells into chronic severe brain injury patients
Yamaki T, Oka S, Iyama S, Sasaki M, Onodera R, Kataoka-Sasaki Y, Namioka T, Namioka A, Nakazaki M, Takemura M, Ukai R, Yokoyama T, Sasaki Y, Yamashita T, Kobayashi M, Yamaguchi M, Fukino M, Takazawa T, Hayasaka M, Owaku T, Funakura M, Onodera S, Ito Y, Kobune M, Kato J, Ishiai S, Kocsis J, Odaki M, Iwadate Y, Kobayashi S, Honmou O. Intravenous infusion of auto-serum-expanded autologous mesenchymal stem cells into chronic severe brain injury patients. Interdisciplinary Neurosurgery 2024, 36: 101927. DOI: 10.1016/j.inat.2023.101927.Peer-Reviewed Original ResearchSevere brain injury patientsAutologous mesenchymal stem cellsBrain injury patientsIntravenous infusionMesenchymal stem cellsAdverse eventsInjury patientsInfused mesenchymal stem cellsHealth Stroke ScaleSerious adverse eventsFugl-Meyer AssessmentInitial case seriesBrain metabolic activityStem cellsMSC infusionStroke ScaleNeurological deteriorationBarthel IndexCase seriesCNS tumorsNeurological functionFunctional statusFunctional improvementChronic patientsPlacebo effect
2023
Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imagingTherapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury
Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis J, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatric Research 2023, 94: 1921-1928. PMID: 37422495, DOI: 10.1038/s41390-023-02717-9.Peer-Reviewed Original ResearchConceptsPerinatal brain injuryBrain injuryMesenchymal stem cellsIntravenous infusionVehicle groupBrain volumeTherapeutic efficacyInfused mesenchymal stem cellsLeft common carotid arteryHistological analysisNon-ischemic hemispherePostnatal day 7Common carotid arteryEmbryonic day 18Stem cellsHypoxia-ischemiaMSC infusionPreterm infantsGABAergic cellsNeurological functionSignificant complicationsCortical synapsesFunctional improvementCarotid arteryIntravenous administrationMesenchymal Stem Cell Transplantation for Spinal Cord Injury: Current Status and Prospects
Hirota R, Sasaki M, Honmou O, Yamashita T. Mesenchymal Stem Cell Transplantation for Spinal Cord Injury: Current Status and Prospects. Spine Surgery And Related Research 2023, 7: 319-326. PMID: 37636138, PMCID: PMC10447197, DOI: 10.22603/ssrr.2022-0234.Peer-Reviewed Original ResearchSpinal cord injuryMesenchymal stem cellsCord injuryMesenchymal stem cell transplantationSevere spinal cord injuryCentral nervous system diseaseMotor function recoveryStem cell transplantationNervous system diseasesSCI modelCell transplantationFunction recoveryIntravenous infusionMSC transplantationMotor functionRat modelIntravenous administrationSystem diseasesBone marrowSCI researchTransplantationInjuryRecent updatesStem cellsCell types
2022
Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model
Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S. Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model. Scientific Reports 2022, 12: 16986. PMID: 36216855, PMCID: PMC9551049, DOI: 10.1038/s41598-022-18485-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowDisease Models, AnimalGreen Fluorescent ProteinsHindlimbInfusions, IntravenousIschemiaLower ExtremityMesenchymal Stem Cell TransplantationMesenchymal Stem CellsNeovascularization, PhysiologicPerfusionPeripheral Vascular DiseasesRatsRats, Sprague-DawleyVascular Endothelial Growth Factor AConceptsIntravenous infusionVascular endothelial growth factorMesenchymal stem cellsTissue perfusionHindlimb ischemia modelRat hindlimb ischemia modelIschemia modelCell groupsFemoral artery removalIschemia animal modelStem cellsHindlimb muscle tissueBone marrow-derived mesenchymal stem cellsEndothelial growth factorMarrow-derived mesenchymal stem cellsMSC infusionHistological findingsIschemic limbsHindlimb ischemiaCell delivery methodsIschemic hindlimbSprague-DawleyCapillary densityAnimal modelsInfusionEnhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projectionsIntravenous Infusion of Autoserum-Expanded Autologous Mesenchymal Stem Cells in Patients With Chronic Brain Injury: Protocol for a Phase 2 Trial
Oka S, Yamaki T, Sasaki M, Ukai R, Takemura M, Yokoyama T, Kataoka-Sasaki Y, Onodera R, Ito YM, Kobayashi S, Kocsis JD, Iwadate Y, Honmou O. Intravenous Infusion of Autoserum-Expanded Autologous Mesenchymal Stem Cells in Patients With Chronic Brain Injury: Protocol for a Phase 2 Trial. JMIR Research Protocols 2022, 11: e37898. PMID: 35793128, PMCID: PMC9301565, DOI: 10.2196/37898.Peer-Reviewed Original ResearchChronic brain injuryAutologous mesenchymal stem cellsSpinal cord injuryINTERNATIONAL REGISTERED REPORT IDENTIFIERBrain injuryIntravenous infusionRankin Scale gradesCord injuryMesenchymal stem cellsClinical trialsScale gradeTherapeutic efficacyJapan Medical Association CenterSapporo Medical University HospitalInvestigator-initiated clinical trialsModified Rankin Scale gradesOpen-label trialPhase 2 studyPhase 2 trialMedical University HospitalCause of disabilityMotor vehicle accidentsPotential therapeutic efficacyProportion of casesStem cells
2021
Intravenous Infusion of Mesenchymal Stem Cells Promotes the Survival of Random Pattern Flaps in Rats
Nakagawa T, Sasaki M, Kataoka-Sasaki Y, Yotsuyanagi T, Radtke C, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Promotes the Survival of Random Pattern Flaps in Rats. Plastic & Reconstructive Surgery 2021, 148: 799-807. PMID: 34550936, DOI: 10.1097/prs.0000000000008327.Peer-Reviewed Original ResearchConceptsMesenchymal stem cell groupStem cell groupIntravenous infusionRandom pattern flapsCell groupsMesenchymal stem cellsFlap survivalHistologic analysisRandom-pattern skin flapsBlood perfusionSurgical reconstruction optionsHigh expressionSkin flap survivalFibroblast growth factor-2 (FGF-2) mRNAQuantitative reverse transcription polymerase chain reactionStem cellsFlap survival areaReverse transcription-polymerase chain reactionSkin flap surgeryExperimental disease modelsSkin blood perfusionSkin flap modelSoft tissue defectsMasson's trichrome stainingMasson's trichrome staining resultsIntravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia
Kiyose R, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Magota H, Oka S, Ukai R, Takemura M, Yokoyama T, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia. World Neurosurgery 2021, 149: e160-e169. PMID: 33618048, DOI: 10.1016/j.wneu.2021.02.056.Peer-Reviewed Original ResearchConceptsRegional cerebral blood flowInfused mesenchymal stem cellsReperfusion therapyPermanent MCAOIntravenous infusionMesenchymal stem cellsTherapeutic efficacyTransient middle cerebral artery occlusion modelMiddle cerebral artery occlusion modelArterial spin labeling magnetic resonance imagingArtery occlusion modelStandard therapeutic strategyTransient MCAO modelCerebral blood flowBehavioral functionsNon-favorable outcomeRat model systemStem cellsMCAO groupStroke inductionAcute strokeCerebral ischemiaNeurologic functionInfusion groupMCAO modelIntravenous infusion of auto serum-expanded autologous mesenchymal stem cells in spinal cord injury patients: 13 case series
Honmou O, Yamashita T, Morita T, Oshigiri T, Hirota R, Iyama S, Kato J, Sasaki Y, Ishiai S, Ito YM, Namioka A, Namioka T, Nakazaki M, Kataoka-Sasaki Y, Onodera R, Oka S, Sasaki M, Waxman SG, Kocsis JD. Intravenous infusion of auto serum-expanded autologous mesenchymal stem cells in spinal cord injury patients: 13 case series. Clinical Neurology And Neurosurgery 2021, 203: 106565. PMID: 33667953, DOI: 10.1016/j.clineuro.2021.106565.Peer-Reviewed Original ResearchConceptsSpinal cord injuryAmerican Spinal Injury Association Impairment ScaleMSC infusionAutologous mesenchymal stem cellsASIA CASIA DIntravenous infusionMesenchymal stem cellsSCI patientsFunctional statusFunctional improvementSpinal cord injury patientsSpinal Cord Independence MeasureASIA B patientsASIA C patientsPhase 2 studySerious adverse eventsCord injury patientsCurrent therapeutic optionsStem cellsASIA BASIA gradeC patientsNeurologic improvementAdverse eventsIntravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model
Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model. Brain Research 2021, 1757: 147296. PMID: 33516815, DOI: 10.1016/j.brainres.2021.147296.Peer-Reviewed Original ResearchConceptsBlood-spinal cord barrierQuantitative reverse transcription polymerase chain reactionIntravenous infusionDisease progressionMotor neuronsMSC groupLocomotor functionOpen-field locomotor functionPreservation of microvasculatureHind limb functionCommon clinical featuresEvans blue leakageMotor neuron lossReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionDevastating neurodegenerative diseaseBBB scoringBSCB functionRotarod testingClinical featuresNeuron lossLimb functionNeurotrophic factorCurative strategiesSpinal cord
2020
Prolonged lifespan in a spontaneously hypertensive rat (stroke prone) model following intravenous infusion of mesenchymal stem cells
Nakazaki M, Oka S, Sasaki M, Kataoka-Sasaki Y, Nagahama H, Hashi K, Kocsis JD, Honmou O. Prolonged lifespan in a spontaneously hypertensive rat (stroke prone) model following intravenous infusion of mesenchymal stem cells. Heliyon 2020, 6: e05833. PMID: 33392407, PMCID: PMC7773587, DOI: 10.1016/j.heliyon.2020.e05833.Peer-Reviewed Original ResearchHypertensive rat modelIntravenous infusionMesenchymal stem cellsRat modelGrowth factor-β1Forkhead box O1Stem cellsRenal dysfunctionMSC infusionSpontaneous hypertensionHypertensive ratsFactor-β1Survival rateTherapeutic efficacyInfusionBox O1Gene expression analysisHypertensionCellsDysfunctionMicrovascularKidneyRatsLiverExpression analysisIntravenous delivery of mesenchymal stem cells protects both white and gray matter in spinal cord ischemia
Yasuda N, Sasaki M, Kataoka-Sasaki Y, Nagahama H, Kocsis JD, Kawaharada N, Honmou O. Intravenous delivery of mesenchymal stem cells protects both white and gray matter in spinal cord ischemia. Brain Research 2020, 1747: 147040. PMID: 32771405, DOI: 10.1016/j.brainres.2020.147040.Peer-Reviewed Original ResearchConceptsIschemic spinal cord injuryIntravenous infusionMesenchymal stem cellsDiffusion tensor imagingGray matterLocomotor functionTherapeutic efficacyHind limb locomotor functionGray matter volume reductionPreservation of microvasculatureSpinal cord ischemiaLeft subclavian arteryPersistent motor deficitsVehicle-treated groupSpinal cord injuryStem cellsBSCB functionISCI ratsAortic surgeryCord ischemiaDevastating complicationNeuronal lossSubclavian arteryMotor deficitsCord injury
2019
Prevention of neointimal hyperplasia induced by an endovascular stent via intravenous infusion of mesenchymal stem cells.
Nakazaki M, Oka S, Sasaki M, Kataoka-Sasaki Y, Onodera R, Komatsu K, Iihoshi S, Hiroura M, Kawaguchi A, Kocsis JD, Honmou O. Prevention of neointimal hyperplasia induced by an endovascular stent via intravenous infusion of mesenchymal stem cells. Journal Of Neurosurgery 2019, 133: 1773-1785. PMID: 31585431, DOI: 10.3171/2019.7.jns19575.Peer-Reviewed Original ResearchSuperficial cervical arteryCommon carotid arteryMarrow-derived mesenchymal stem cellsIntravenous infusionNeointimal hyperplasiaIschemic eventsInflammatory responseStent restenosisStent strutsMesenchymal stem cellsPorcine modelHuman middle cerebral arteryEndovascular stentsCerebral ischemic eventsPercutaneous transluminal angioplastyMiddle cerebral arteryBare metal stentsBone marrow-derived mesenchymal stem cellsDeployment of stentsStem cellsAngiographic imagesIntravascular ultrasound imagingTransluminal angioplastyCerebral arteryCerebral strokeIntravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model
Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Suzuki J, Sasaki Y, Nagahama H, Hashi K, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model. Neuroscience 2019, 408: 361-377. PMID: 30999031, DOI: 10.1016/j.neuroscience.2019.04.018.Peer-Reviewed Original ResearchConceptsCerebral small vessel diseaseBlood-brain barrierProgressive brain atrophyVascular dementiaMesenchymal stem cellsAlzheimer's diseaseCognitive functionBrain atrophyIntravenous infusionEffects of MSCsInfused mesenchymal stem cellsSmall vessel diseaseEssential pathological featuresAccumulation of AβSpinal cord injuryBBB transport systemsStem cellsBBB functionAβ accumulationHypertensive ratsNeuronal damageFunctional recoveryVessel diseaseCord injuryPathological features
2018
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a rat model of chronic cerebral infarction.
Namioka T, Namioka A, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a rat model of chronic cerebral infarction. Journal Of Neurosurgery 2018, 131: 1289-1296. PMID: 30485210, DOI: 10.3171/2018.5.jns18140.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionCerebral infarctionIntravenous infusionMesenchymal stem cellsChronic phaseRat modelBone marrowBlood-brain barrier integrityCerebral artery occlusionVehicle-treated groupChronic cerebral infarctionPhase of strokeStem cellsAdult bone marrowArtery occlusionBBB leakageMotor recoveryFunctional recoveryIschemic volumeFunctional outcomeSystemic infusionBrain parenchymaFunctional improvementSystemic injectionClinical studiesIntravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat.
Namioka A, Namioka T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat. Journal Of Neurosurgery 2018, 131: 1308-1316. PMID: 30485204, DOI: 10.3171/2018.4.jns173121.Peer-Reviewed Original ResearchBasilar artery occlusionIschemic lesion volumeInfused mesenchymal stem cellsIntravenous infusionMesenchymal stem cellsLesion volumeBrainstem infarctionMSC infusionFunctional recoveryIntravenous MSC infusionIschemic brainstem lesionsPosterior circulation strokeAcute stroke therapyArtery occlusion modelGroup 28 daysNylon monofilament sutureReliable animal modelMultiple behavioral testsStem cellsProximal BAArtery occlusionBrainstem lesionsPosterior circulationStroke therapyFunctional outcomeIntravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury
Oshigiri T, Sasaki T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Oka S, Morita T, Hirota R, Yoshimoto M, Yamashita T, Hashimoto-Torii K, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury. Journal Of Neurotrauma 2018, 36: 411-420. PMID: 29901416, PMCID: PMC6352512, DOI: 10.1089/neu.2018.5793.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfused mesenchymal stem cellsMesenchymal stem cellsCord injuryIntravenous infusionFunctional improvementAcute spinal cord injuryBlood-spinal cord barrierGene expression signaturesAxonal sproutingFunctional recoveryMotor cortexSystemic infusionVehicle infusionSpinal cordRat modelTherapeutic mechanismImproved functionInfusionTranscription-polymerase chain reaction dataPearson correlation analysisBehavioral functionsExpression signaturesGenome-wide RNA profilingRecent evidencePreservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction
Nagahama H, Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Namioka T, Namioka A, Oka S, Onodera R, Suzuki J, Sasaki Y, Kocsis JD, Honmou O. Preservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction. Brain Research 2018, 1695: 37-44. PMID: 29802840, DOI: 10.1016/j.brainres.2018.05.033.Peer-Reviewed Original ResearchConceptsInterhemispheric cortical connectionsCorpus callosumIntravenous infusionMesenchymal stem cellsDiffusion tensor imagingMotor cortexFunctional improvementCortical connectionsAdeno-associated virusRat middle cerebral artery occlusion stroke modelMiddle cerebral artery occlusion stroke modelContralateral motor cortexBlood-brain barrierMotor cortex connectivityVehicle-infused groupMRI diffusion tensor imagingPotential therapeutic mechanismNeuroanatomical tracing techniquesStem cellsCerebral infarctionInflammatory infiltrationAnatomical restorationCerebral strokeFunctional recoveryNeurotrophic factorIntravenous infusion of mesenchymal stem cells reduces epileptogenesis in a rat model of status epilepticus
Fukumura S, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Nagahama H, Morita T, Sakai T, Tsutsumi H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells reduces epileptogenesis in a rat model of status epilepticus. Epilepsy Research 2018, 141: 56-63. PMID: 29475054, DOI: 10.1016/j.eplepsyres.2018.02.008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDisease Models, AnimalGlutamate DecarboxylaseGreen Fluorescent ProteinsHippocampusInfusions, IntravenousLithiumMagnetic Resonance ImagingMaleMaze LearningMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMuscarinic AgonistsNeuronsPhosphopyruvate HydratasePilocarpineRatsRats, Sprague-DawleyRats, TransgenicStatus EpilepticusTime FactorsConceptsAberrant mossy fiber sproutingMossy fiber sproutingStatus epilepticusNeuronal cell deathMesenchymal stem cellsMSC infusionIntravenous infusionRat modelCognitive functionMorris water maze testCognitive function preservationNumber of GAD67Water maze testVehicle-infused ratsMagnetic resonance imagingCell deathStem cellsSeizure frequencyFiber sproutingFunction preservationTimm stainingMaze testHippocampal neuronsImmunohistochemical stainingCognitive deterioration